Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,701,213	T→G	L151R (CTG→CGG)	proW →	glycine betaine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,701,213	0	T	G	100.0%	202.9 / NA	49	L151R (CTG→CGG)	proW	glycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/28);  total (21/28)

TGGCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCG  >  REL606/2701141‑2701283                                                                         |                                                                       tgGCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCgggg                                                                     >  2:1574671/1‑75 (MQ=255)  ggCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGgtg                                                                    >  2:1392827/1‑76 (MQ=255)    cGACGCTGGTTTCGCGGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGtt                                                                  <  2:993530/76‑1 (MQ=255)      aCGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTaa                                                                <  2:1191904/76‑1 (MQ=255)      aCGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTaa                                                                >  1:1913124/1‑76 (MQ=255)          tGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGc                                                            >  1:1035979/1‑76 (MQ=255)          tGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGc                                                            >  1:1558917/1‑76 (MQ=255)          tGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGc                                                            >  1:1619737/1‑76 (MQ=255)             tttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCct                                                         >  2:699865/1‑76 (MQ=255)             tttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCct                                                         >  2:1433641/1‑76 (MQ=255)             tttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCct                                                         <  1:393899/76‑1 (MQ=255)             tttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCct                                                         <  1:341570/76‑1 (MQ=255)              ttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctg                                                        >  1:1480492/1‑76 (MQ=255)              ttCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctg                                                        <  2:68766/76‑1 (MQ=255)              gtCGCTGATTGCCATCGGCGCAATCGGTGCCGGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctg                                                        <  2:1194009/75‑1 (MQ=255)               tCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctgc                                                       >  1:263968/1‑76 (MQ=255)                 gCTGATTGCCATCGGCGCAGTCGTTGCCTGGTCGTAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctgctg                                                     <  2:526960/76‑1 (MQ=255)                 gCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCctgctg                                                     <  2:1864280/76‑1 (MQ=255)                  cTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGt                                                    >  1:765804/1‑76 (MQ=255)                    gATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTc                                                  >  1:256220/1‑76 (MQ=255)                      ttGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTg                                                <  2:256220/76‑1 (MQ=255)                      ttGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTg                                                <  1:1292701/76‑1 (MQ=255)                        gCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTa                                              <  1:1592759/76‑1 (MQ=255)                         ccATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTAt                                             <  2:647768/76‑1 (MQ=255)                              ggCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCa                                        >  2:518222/1‑76 (MQ=255)                              ggCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCa                                        <  2:1910832/76‑1 (MQ=255)                               gcgcAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCAt                                       <  2:1427183/76‑1 (MQ=255)                                   aaTCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGt                                   >  2:18713/1‑76 (MQ=255)                                   aaTCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGt                                   >  2:187444/1‑76 (MQ=255)                                   aaTCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGt                                   >  1:1071984/1‑76 (MQ=255)                                   aaTCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGt                                   <  2:1368975/76‑1 (MQ=255)                                      cGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTg                                >  2:1664897/1‑76 (MQ=255)                                      cGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTg                                <  2:2028340/76‑1 (MQ=255)                                         tGCCTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCg                             <  1:1497216/76‑1 (MQ=255)                                           ccTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGtt                           <  2:1275626/76‑1 (MQ=255)                                           ccTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGtt                           <  2:668587/76‑1 (MQ=255)                                            cTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTg                          >  1:454683/1‑76 (MQ=255)                                            cTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTg                          >  1:818725/1‑76 (MQ=255)                                            cTGGTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTg                          <  2:1608491/76‑1 (MQ=255)                                               gTCGCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTgggg                       <  1:272497/76‑1 (MQ=255)                                                  gCAGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGata                    <  2:877183/76‑1 (MQ=255)                                                   cAGGCAATGGTGTCTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGatat                   <  1:844043/76‑1 (MQ=255)                                                    aGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATAtg                  >  2:922092/1‑76 (MQ=255)                                                     ggCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATAtgg                 <  1:1468519/76‑1 (MQ=255)                                                      gCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATAtggc                <  2:1796135/76‑1 (MQ=255)                                                        aaTGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATAtggctg              <  2:1220230/76‑1 (MQ=255)                                                          tGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATAtggctggc            <  1:1489528/76‑1 (MQ=255)                                                           ggTGGCTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCg           <  1:343034/76‑1 (MQ=255)                                                            gTGACTCTGGCGCGGGTTTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCga          <  2:1811679/76‑1 (MQ=255)                                                              gACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGGgaga        >  1:1928465/1‑76 (MQ=255)                                                              gACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCgaga        >  1:1454566/1‑76 (MQ=255)                                                               aCTGTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGaa       <  2:1488147/76‑1 (MQ=255)                                                                    ggCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCcg  <  2:356272/76‑1 (MQ=255)                                                                         |                                                                       TGGCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCG  >  REL606/2701141‑2701283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑