Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,202,632	T→C	intergenic (‑73/‑127)	iclR ← / → metH	DNA‑binding transcriptional repressor/B12‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,202,632	0	T	C	100.0%	267.6 / NA	61	intergenic (‑73/‑127)	iclR/metH	DNA‑binding transcriptional repressor/B12‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/27);  total (34/27)

GACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGC  >  REL606/4202560‑4202703                                                                         |                                                                        gACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGAt                                                                      <  2:1011201/76‑1 (MQ=255)  aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATc                                                                     <  2:1846892/76‑1 (MQ=255)     gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGa                                                                  <  2:1086726/76‑1 (MQ=255)      tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAAccgatccgat                                                                 <  1:369083/76‑1 (MQ=255)      tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAAccgatccgat                                                                 <  2:1664698/76‑1 (MQ=255)      tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAAccgatccgat                                                                 <  2:1562864/76‑1 (MQ=255)       ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGATCCGATCCGATg                                                                <  2:62665/76‑1 (MQ=255)       ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATg                                                                <  2:194648/76‑1 (MQ=255)       ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATg                                                                <  2:1935953/76‑1 (MQ=255)       ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATg                                                                >  1:1593257/1‑76 (MQ=255)       ctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATg                                                                <  2:1178211/76‑1 (MQ=255)        tcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGa                                                               >  2:763618/1‑76 (MQ=255)        tcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGa                                                               <  2:1090927/76‑1 (MQ=255)             tttCTGTATCGTGGTAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTaa                                                          <  1:1248331/76‑1 (MQ=255)               tCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATg                                                        <  1:1075364/76‑1 (MQ=255)               tCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATg                                                        >  2:912646/1‑76 (MQ=255)                cTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGt                                                       >  1:1879415/1‑76 (MQ=255)                cTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGt                                                       >  1:652779/1‑76 (MQ=255)                 tGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGtt                                                      <  2:1633798/76‑1 (MQ=255)                    aTCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGaa                                                   >  2:1326740/1‑76 (MQ=255)                    aTCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGaa                                                   <  1:344202/76‑1 (MQ=255)                     tCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAAc                                                  <  2:2023196/76‑1 (MQ=255)                     tCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAAc                                                  <  1:1126133/76‑1 (MQ=255)                      cGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACa                                                 >  1:2026448/1‑76 (MQ=255)                      cGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACa                                                 >  1:1611078/1‑76 (MQ=255)                        tGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACaaa                                               >  1:1372658/1‑76 (MQ=255)                           aaaTCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAAtct                                            <  1:1883534/76‑1 (MQ=255)                           aaaTCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAAtct                                            <  2:780954/76‑1 (MQ=255)                            aaTCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAAtctc                                           <  1:195069/76‑1 (MQ=255)                             aTCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCa                                          >  1:1342842/1‑76 (MQ=255)                              tcattttcatttTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCAt                                         <  2:1977983/76‑1 (MQ=255)                              tcattttcatttTTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCAt                                         <  1:400778/76‑1 (MQ=255)                                   ttcattttTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                    >  1:1835543/1‑76 (MQ=255)                                     cattttTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgt                                  >  1:754926/1‑76 (MQ=255)                                     cattttTATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgt                                  >  1:1904293/1‑76 (MQ=255)                                       tttttATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgtgg                                <  1:1365357/76‑1 (MQ=255)                                          ttATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGtggtgg                             <  1:1921719/76‑1 (MQ=255)                                          ttATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGtggtgg                             >  1:571408/1‑76 (MQ=255)                                          ttATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGtggtgg                             >  1:1621324/1‑76 (MQ=255)                                           tATTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGGGTTAATGTTGAACAAATCTCATGTTGCGTggtggt                            >  2:1142894/1‑76 (MQ=255)                                            aTTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTc                           >  1:544881/1‑76 (MQ=255)                                            aTTGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTc                           >  2:390124/1‑76 (MQ=255)                                             ttGTTAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCg                          >  1:1268752/1‑76 (MQ=255)                                                ttAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtt                       >  2:1706795/1‑76 (MQ=255)                                                 tAGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCttt                      >  1:1755803/1‑76 (MQ=255)                                                  aGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtttt                     >  1:220555/1‑76 (MQ=255)                                                  aGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtttt                     >  1:216144/1‑76 (MQ=255)                                                  aGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtttt                     <  1:464060/76‑1 (MQ=255)                                                  aGCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtttt                     <  2:865957/76‑1 (MQ=255)                                                   gCTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTa                    >  1:1685594/1‑76 (MQ=255)                                                    cTAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTAc                   >  2:2042281/1‑76 (MQ=255)                                                     tAATGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTAcc                  >  1:1255575/1‑76 (MQ=255)                                                        tGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACcaca               >  2:32313/1‑76 (MQ=255)                                                        tGCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACcaca               <  1:1745111/76‑1 (MQ=255)                                                         gCAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAg              <  1:1429641/76‑1 (MQ=255)                                                          cAATAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGa             >  2:1609992/1‑76 (MQ=255)                                                           aaTAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGAt            <  2:1444548/76‑1 (MQ=255)                                                            aTAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg           >  1:2036903/1‑76 (MQ=255)                                                            aTAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg           >  2:987574/1‑76 (MQ=255)                                                             tAGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGc          >  2:68584/1‑76 (MQ=255)                                                              aGTTGCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCg         <  1:869866/76‑1 (MQ=255)                                                                  gCTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTa     >  2:12363/1‑76 (MQ=255)                                                                   cTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTAt    >  1:437651/1‑76 (MQ=255)                                                                     gAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGc  >  2:944502/1‑76 (MQ=255)                                                                         |                                                                        GACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTGCTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGC  >  REL606/4202560‑4202703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑