| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 649,601 | T→C | I401V (ATC→GTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 649,601 | 0 | T | C | 95.2% | 156.3 / ‑0.3 | 42 | I401V (ATC→GTC) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (18/22); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01 | |||||||||||
TCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG > REL606/649531‑649673 | tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa > 1:374083/1‑76 (MQ=255)tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa > 1:10111/1‑76 (MQ=255) cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat < 2:260355/76‑1 (MQ=255) cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat < 2:1554226/76‑1 (MQ=255) cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat > 2:1957516/1‑76 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat < 2:1764230/76‑1 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat > 1:620001/1‑76 (MQ=255) ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc < 1:1956566/76‑1 (MQ=255) cGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCg < 1:1667747/76‑1 (MQ=255) aGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAgg < 1:1536709/76‑1 (MQ=255) tCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCa > 1:1203804/1‑76 (MQ=255) cGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCAc > 1:350263/1‑76 (MQ=255) gATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCAcc > 2:598181/1‑76 (MQ=255) aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCt < 1:234313/76‑1 (MQ=255) cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATa < 1:1617373/76‑1 (MQ=255) gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATaga < 2:1165732/76‑1 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa < 1:1608929/76‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaag > 1:917083/1‑76 (MQ=255) tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga > 2:811615/1‑76 (MQ=255) tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga > 2:1275826/1‑76 (MQ=255) cATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc > 1:2050376/1‑76 (MQ=255) cATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc < 2:2003542/76‑1 (MQ=255) aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc < 1:1909920/76‑1 (MQ=255) aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGGATcc > 1:728689/1‑76 (MQ=255) aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc > 1:684574/1‑76 (MQ=255) aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc < 1:1753520/76‑1 (MQ=255) aaTTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt < 2:1697342/76‑1 (MQ=255) aaTTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt > 2:341822/1‑76 (MQ=255) tttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct < 1:1684950/76‑1 (MQ=255) tttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct < 2:835009/76‑1 (MQ=255) aCCCATACATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTcc > 2:1495012/1‑76 (MQ=255) cccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCa < 2:184252/76‑1 (MQ=255) cccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGCAGCAGCAGCTTCTTCCa > 1:1986585/1‑76 (MQ=255) ccatccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAg < 2:192474/76‑1 (MQ=255) tccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct < 2:44325/68‑1 (MQ=255) tccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct > 1:44325/1‑68 (MQ=255) catTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc > 1:14715/1‑76 (MQ=255) ttCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCtt > 1:422754/1‑76 (MQ=255) ttCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCtt < 1:797248/76‑1 (MQ=255) agGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAt < 2:31901/76‑1 (MQ=255) agGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAt > 2:571839/1‑76 (MQ=255) gCGATCGCCCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTc < 1:690449/76‑1 (MQ=255) gCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACangc < 2:1448235/76‑4 (MQ=255) cgatcgacCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCa < 2:1650517/76‑1 (MQ=255) atcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGa > 2:456044/1‑76 (MQ=255) tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGAc < 1:1368779/76‑1 (MQ=255) tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGAATCCGCAGATTCTTCCAGCGCTCTTGCGACATTCAGAc > 2:187317/1‑76 (MQ=255) cgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACg > 2:1765494/1‑76 (MQ=255) | TCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG > REL606/649531‑649673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |