| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,266,142 | C→T | Y327Y (TAC→TAT) | prfA → | peptide chain release factor 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,266,142 | 0 | C | T | 98.2% | 202.9 / ‑4.0 | 54 | Y327Y (TAC→TAT) | prfA | peptide chain release factor 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (29/24); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GCGACCGTAACCGTACTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGAC > REL606/1266072‑1266214 | gCGACCGTAACCGTACTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCt < 2:112141/76‑1 (MQ=255) gACCGTAACCGTACTTACAACTTCCCACAGGGGCGCGCTACCGGCCCCCCCAACAACCTGTCGCTCTATCGCCTgg > 1:1126209/1‑76 (MQ=255) gccgtaaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGa < 2:979107/75‑1 (MQ=255) ccgtaaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGAt < 1:460314/76‑1 (MQ=255) cgtaaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATg < 1:640179/76‑1 (MQ=255) cgtaaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATg > 1:40996/1‑76 (MQ=255) gtaaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGa > 2:1703182/1‑76 (MQ=255) taaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGaa > 1:1238490/1‑76 (MQ=255) taaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGaa < 1:755724/76‑1 (MQ=255) taaccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGaa > 1:194851/1‑76 (MQ=255) accgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGt < 1:348760/76‑1 (MQ=255) ccgtaCTTACAACTTCCCCCAGGGGCGCGTTGCCGATCTCCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTg < 2:1645430/76‑1 (MQ=255) ccgtaCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTg < 2:709784/76‑1 (MQ=255) aCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATgg > 1:670449/1‑76 (MQ=255) aCTTACAACTTCCCACAGGGGCGCGTTACCGATCACCCCATCAACCTGACGCTCTATCGCCTGGGTGAAGCGGTgg > 1:2026416/1‑76 (MQ=255) tACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAg > 1:412879/1‑76 (MQ=255) aaCTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTa > 1:1083711/1‑76 (MQ=255) aaCTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTa < 2:1940377/76‑1 (MQ=255) aaCTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTa > 1:2059886/1‑76 (MQ=255) aaCTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTa < 1:2027784/76‑1 (MQ=255) tCCCACAGGGGCGCGGTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCt < 1:1890844/76‑1 (MQ=255) ccACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTgg < 1:538518/76‑1 (MQ=255) ccACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGGAAGCTgg > 1:1137587/1‑76 (MQ=255) acaGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGat < 2:1547/76‑1 (MQ=255) aGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGatat < 1:1818569/76‑1 (MQ=255) ggCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCt < 2:1529057/76‑1 (MQ=255) ggCGCGTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCt < 1:1132957/76‑1 (MQ=255) cgTTACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGAtt > 2:341150/1‑76 (MQ=255) gTTATCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTAGATATGCTGATTg > 2:1331926/1‑76 (MQ=255) ttACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGa > 1:337526/1‑76 (MQ=255) ttACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGa > 1:508595/1‑76 (MQ=255) tACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGaa > 1:1983525/1‑76 (MQ=255) tACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGaa > 1:1837196/1‑76 (MQ=255) tACCGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGaa > 1:129436/1‑76 (MQ=255) ccGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAAcc > 1:220666/1‑76 (MQ=255) ccGATCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAAcc > 2:1881071/1‑76 (MQ=255) aTCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGAt > 1:2061798/1‑76 (MQ=255) aTCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGAt > 1:1390817/1‑76 (MQ=255) aTCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGAt > 1:1898399/1‑76 (MQ=255) aTCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGAt > 2:1013109/1‑76 (MQ=255) aTCACCGCATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGAt < 1:968556/76‑1 (MQ=255) cACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTa < 2:1420230/76‑1 (MQ=255) cATCACCCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAg < 1:1176542/76‑1 (MQ=255) cATCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAg > 2:813615/1‑76 (MQ=255) cANCAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAg > 2:1447193/1‑76 (MQ=255) cAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGaa > 1:1333994/1‑76 (MQ=255) cAACCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGaa < 2:1397513/76‑1 (MQ=255) aaCCTGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAAc > 2:734220/1‑76 (MQ=255) tGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCa < 2:1550657/76‑1 (MQ=255) tGACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCa < 1:774105/76‑1 (MQ=255) gACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAg < 1:923563/76‑1 (MQ=255) gACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAg < 2:910181/76‑1 (MQ=255) gACGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAg > 2:403524/1‑76 (MQ=255) gACGCACTGACGCCTGGATTAAGTGGTGGAAGGTAAGCTGGATATGCTGATTGTACCGGTTATCCAGGAACATCAg < 2:30318/76‑1 (MQ=255) aCGCTCTATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAgg < 2:2026416/76‑1 (MQ=255) tctATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGa > 1:1167541/1‑76 (MQ=255) ctATCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGAc > 1:1797257/1‑76 (MQ=255) ctATCGCCTGGATGAAGTGATGGAAGGGAAGCTGGATATGCTGGTTTAACCGATTATCCAGGAAAATCAGGCCGAc > 2:1748548/1‑76 (MQ=255) | GCGACCGTAACCGTACTTACAACTTCCCACAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGAC > REL606/1266072‑1266214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |