Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,304,883	A→G	Y453H (TAT→CAT)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,304,883	0	A	G	100.0%	162.4 / NA	39	Y453H (TAT→CAT)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAG  >  REL606/1304810‑1304955                                                                          |                                                                         cccTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGAt                                                                        <  1:1065242/74‑1 (MQ=255)  gCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATc                                                                       <  2:1139913/76‑1 (MQ=255)   cTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCg                                                                      <  1:114330/76‑1 (MQ=255)   cTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCg                                                                      >  2:100953/1‑76 (MQ=255)    tCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGt                                                                     <  1:1669816/76‑1 (MQ=255)     cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTc                                                                    <  1:1903036/76‑1 (MQ=255)     cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTc                                                                    >  1:1813597/1‑76 (MQ=255)       gCGACACGTTGCCATAAGGGACGTTTTAGCCATGAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCt                                                                  <  2:98890/76‑1 (MQ=255)          acacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGaa                                                               >  1:1972743/1‑76 (MQ=255)          acacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGaa                                                               >  1:1655759/1‑76 (MQ=255)                    aTAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAg                                                     >  2:1652906/1‑76 (MQ=255)                    aTAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAg                                                     >  2:1249031/1‑76 (MQ=255)                     tAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAgg                                                    >  2:632387/1‑76 (MQ=255)                        tGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCt                                                 <  1:797535/76‑1 (MQ=255)                        cggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGGCt                                                 >  2:195096/2‑76 (MQ=255)                         ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTg                                                <  2:1802682/76‑1 (MQ=255)                         ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTg                                                >  1:1088170/1‑76 (MQ=255)                         ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGCCTg                                                >  1:1473362/1‑76 (MQ=255)                           aCGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCa                                              <  1:1368658/76‑1 (MQ=255)                            cGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCAc                                             <  2:525690/76‑1 (MQ=255)                              ttttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCa                                           <  2:865019/76‑1 (MQ=255)                              ttttACCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCa                                           <  2:975108/76‑1 (MQ=255)                                ttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCaaa                                         <  1:1811273/76‑1 (MQ=255)                                  aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAAc                                       >  1:1948688/1‑76 (MQ=255)                                   gCCATAAACGGGCATCGAGCAGACGTGAACTCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAAcc                                      <  2:1102840/76‑1 (MQ=255)                                     cATAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCtt                                    <  1:605795/76‑1 (MQ=255)                                      aTAAACGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCttt                                   <  1:340490/76‑1 (MQ=255)                                        aaaCGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTAt                                 <  2:1039304/76‑1 (MQ=255)                                        aaaCGGGCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTAt                                 >  2:368530/1‑76 (MQ=255)                                             ggCATCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTAtcgtcg                            <  2:1479436/76‑1 (MQ=255)                                                aTCGAGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGAtt                         >  2:1969515/1‑76 (MQ=255)                                                    aGCAGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCa                     <  2:890477/76‑1 (MQ=255)                                                       aGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCagag                  >  1:1394982/1‑76 (MQ=255)                                                       aGACGTGAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCagag                  >  2:869374/1‑76 (MQ=255)                                                             gAACGCGAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAAtct            <  1:1047310/76‑1 (MQ=255)                                                                 gcgAAATATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGaa        <  2:1571381/76‑1 (MQ=255)                                                                    aaaTATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTAGAAAtt     >  2:814325/1‑76 (MQ=255)                                                                     aaTATGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAttt    >  2:818543/1‑76 (MQ=255)                                                                      atatGATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGGAATCTCGAAATTTa   >  2:696435/1‑76 (MQ=255)                                                                       tatgATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAg  <  2:1658184/76‑1 (MQ=255)                                                                       tatgATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAg  >  1:703030/1‑76 (MQ=255)                                                                          |                                                                         CGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAG  >  REL606/1304810‑1304955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑