| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 1733288 = | 7 (0.210) | 32 (0.970) | 26/146 | 0.3 | 86.7% | coding (324/1413 nt) | pykF | pyruvate kinase |
| ? | REL606 | = 1733295 | 3 (0.090) | coding (331/1413 nt) | pykF | pyruvate kinase | |||||
TTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGA > REL606/1733213‑1733362 | ttCTCTGAAAGGTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTa < 2:68287/76‑1 (MQ=255)ttCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTa < 2:322423/76‑1 (MQ=255)aagctggtcagacctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatga > 1:1046474‑M1/76‑76 (MQ=255) nctctGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTaa < 1:942553/75‑1 (MQ=255) agctggtcagacctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaa > 1:1007588‑M1/75‑76 (MQ=255) tctGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACg < 1:330244/76‑1 (MQ=255) ctgttcanacctttactttcaccactgataaatctcttatcggcaacagcgaaatggttgcggtaacgtatgaacg < 1:950757‑M1/4‑1 (MQ=255) ctggtcagacttttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaacg < 1:139644‑M1/4‑1 (MQ=255) ctggtcagacctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaacg > 2:290708‑M1/73‑76 (MQ=255) ctGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGt < 1:857969/76‑1 (MQ=255) ggtcagacctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaacgta < 1:249088‑M1/6‑1 (MQ=255) gAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTAt > 1:480843/1‑76 (MQ=255) aaaGCTGGTCAGACCTTTACTTTCACCACTGATGAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATg < 1:857225/76‑1 (MQ=255) cagacctttactttcaccactgataaatctgttatcggcaacagcgaaanggttgcggtaacgtatgaacgtaaga > 2:935818‑M1/68‑73 (MQ=255) aaGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGa > 1:1046474‑M2/1‑75 (MQ=255) aGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaa > 1:1007588‑M2/1‑74 (MQ=255) gacctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAg > 2:36902‑M1/66‑76 (MQ=255) cTGTTCANACCTTTACTTTCACCACTGATAAATCTCTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacg < 1:950757‑M2/76‑5 (MQ=255) cTGGTCAGACTTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacg < 1:139644‑M2/76‑5 (MQ=255) cTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacg > 2:290708‑M2/1‑72 (MQ=255) ggTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgta < 1:249088‑M2/76‑7 (MQ=255) ctttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGtt < 2:305530‑M1/14‑1 (MQ=255) ttaactttcaccactgataaatctgtnatcggcaacagcgaaatggtagcggtaacgtatgAACGTATGAAGCttt < 2:935193‑M1/15‑1 (MQ=255) ttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAANGTTTc < 2:872437‑M1/16‑1 (MQ=255) ttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTc < 1:682816‑M1/16‑1 (MQ=255) ttactttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTc > 2:79456‑M1/61‑76 (MQ=255) cAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAANGGTTGCGGTAACGTATGaacgtaaga > 2:935818‑M2/1‑67 (MQ=255) gACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaag > 2:36902‑M2/1‑65 (MQ=255) tttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCacta > 1:375564‑M1/57‑76 (MQ=255) ttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCactac < 2:105362‑M1/21‑1 (MQ=255) cTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtt < 2:305530‑M2/76‑15 (MQ=255) ttaACTTTCACCACTGATAAATCTGTNATCGGCAACAGCGAAATGGTAGCGGTAACGTATGaacgtatgaagcttt < 2:935193‑M2/76‑16 (MQ=255) caccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTg < 1:1008757‑M1/23‑1 (MQ=255) ttACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaangtttc < 2:872437‑M2/76‑17 (MQ=255) ttACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttc < 1:682816‑M2/76‑17 (MQ=255) ttACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttc > 2:79456‑M2/1‑60 (MQ=255) ccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGAc > 2:453923‑M1/52‑76 (MQ=255) actgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACct < 1:477808‑M1/27‑1 (MQ=255) tttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcacta > 1:375564‑M2/1‑56 (MQ=255) ttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactac < 2:105362‑M2/76‑22 (MQ=255) tgataaatctgttatcggcaacagngaaatggttgcggtaacgtatgAACGTACGAAGGTTTCACTACTGACctgt < 2:179603‑M1/29‑1 (MQ=255) caccacTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactg < 1:1008757‑M2/76‑24 (MQ=255) ccacTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgac > 2:453923‑M2/1‑51 (MQ=255) atctgttatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg > 1:561939‑M1/42‑76 (MQ=255) acTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacct < 1:477808‑M2/76‑28 (MQ=255) tGATAAATCTGTTATCGGCAACAGNGAAATGGTTGCGGTAACGTATGaacgtacgaaggtttcactactgacctgt < 2:179603‑M2/76‑30 (MQ=255) gttatcggcaccagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCaa < 1:522019‑M1/39‑1 (MQ=255) tatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAaca > 2:844782‑M1/36‑76 (MQ=255) tatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAaca < 1:103507‑M1/41‑1 (MQ=255) tatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAaca < 1:464849‑M1/41‑1 (MQ=255) tatcggcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAaca < 1:516961‑M1/41‑1 (MQ=255) aTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttg > 1:561939‑M2/1‑41 (MQ=255) gcaacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTa < 2:996815‑M1/46‑1 (MQ=255) gTTATCGGCACCAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaa < 1:522019‑M2/76‑40 (MQ=255) aacagcgaaatggttgcggtaacgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACt < 1:822208‑M1/48‑1 (MQ=255) tATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaaca < 1:516961‑M2/76‑42 (MQ=255) tATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaaca < 1:464849‑M2/76‑42 (MQ=255) tATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaaca < 1:103507‑M2/76‑42 (MQ=255) tATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaaca > 2:844782‑M2/1‑35 (MQ=255) acagcgcaatggttgcggtaacgcatgAACGTATGAAGGTTTCACTGCTGACCTGTCTGTTGGCAACACCGTACTg > 1:936811‑M1/28‑76 (MQ=255) gCAACAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaacaccgta < 2:996815‑M2/76‑47 (MQ=255) aaatggttgcggtaacgtatgAACNTATGAAGGTTTCACTACTGACCTGTCTGTAGGCAACACCGTACTGGTTGAc < 2:186996‑M1/55‑1 (MQ=255) aaCAGCGAAATGGTTGCGGTAACGTATGaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtact < 1:822208‑M2/76‑49 (MQ=255) aCAGCGCAATGGTTGCGGTAACGCATGaacgtatgaaggtttcactgctgacctgtctgttggcaacaccgtactg > 1:936811‑M2/1‑27 (MQ=255) aaaTGGTTGCGGTAACGTATGaacntatgaaggtttcactactgacctgtctgtaggcaacaccgtactggttgac < 2:186996‑M2/76‑56 (MQ=255) acgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCgg < 2:470901‑M1/69‑1 (MQ=255) cgtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGt < 1:75139‑M1/70‑1 (MQ=255) gtatgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGgtn < 1:925776‑M1/71‑2 (MQ=255) atgAACGTATGAAGGTTNCACTACTGACCTGTCTGTTGGCAACACCGTACTNCTTGACCATAGACTGATCGGTATg < 2:889913‑M1/73‑1 (MQ=255) gAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGa > 2:276454‑M1/2‑76 (MQ=255) | TTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGA > REL606/1733213‑1733362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |