| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | REL606 | 968,327 | IS150 (–) +2 bp :: +AT | intergenic (‑154/+37) | pflA ← / ← pflB | pyruvate formate lyase activating enzyme 1/pyruvate formate lyase I |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 664687 = | NA (NA) | 38 (1.140) +T |
31/148 | 0.1 | 97.4% | intergenic (‑78/‑48) | gltL/insJ‑2 | ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein |
| ? | REL606 | 968327 = | 1 (0.030) | intergenic (‑154/+38) | pflA/pflB | pyruvate formate lyase activating enzyme 1/pyruvate formate lyase I | |||||
| * | ? | REL606 | = 666130 | NA (NA) | 53 (1.570) | 35/150 | 0.1 | 98.1% | noncoding (1443/1443 nt) | IS150 | repeat region |
| ? | REL606 | = 968328 | 1 (0.030) | intergenic (‑155/+37) | pflA/pflB | pyruvate formate lyase activating enzyme 1/pyruvate formate lyase I | |||||
GTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTG > REL606/968253‑968403 | caaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaattt < 2:410972‑M2/2‑1 (MQ=255) tCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCGTTTATTt > 1:894487‑M2/1‑75 (MQ=255) cACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTCAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtg < 1:854777‑M2/76‑3 (MQ=255) aaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacactTTGt > 2:47912‑M2/73‑76 (MQ=255) ccTTAAAGTATAGATATCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgta > 2:441625‑M2/1‑72 (MQ=255) ttAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtact < 1:297860‑M2/76‑7 (MQ=255) tAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactg > 2:144313‑M2/1‑69 (MQ=255) aaaGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactga < 2:529507‑M2/76‑9 (MQ=255) aaGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgac > 2:379112‑M2/1‑67 (MQ=255) tttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCttttt < 1:651497‑M2/13‑1 (MQ=255) tttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCttttt < 2:66752‑M2/13‑1 (MQ=255) gTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccc > 2:1006181‑M2/1‑65 (MQ=255) tataGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgacccc < 2:547349‑M2/76‑13 (MQ=255) tggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTNTTTAc > 2:873511‑M2/62‑76 (MQ=255) ggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACt > 1:1037581‑M2/61‑76 (MQ=255) ggctttgacatattaggggctattccatttcatcgtccaacaaaatggatgcagtacaatTTGTACGCTTTTTACt > 1:651471‑M2/61‑76 (MQ=255) gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGAGGAGCCTTTATTtgtactgaccccaaaa < 2:492144‑M2/76‑17 (MQ=255) ctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcantacaatTTGTACGCTTTTTACTGt < 2:183364‑M2/18‑1 (MQ=255) ttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTAc > 1:282819‑M2/57‑76 (MQ=255) cTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttg < 2:745891‑M2/76‑22 (MQ=255) acatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGAt > 2:438274‑M2/54‑76 (MQ=255) acatataaggggttattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGAt > 2:131337‑M2/54‑76 (MQ=255) tGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttgg < 2:883337‑M2/76‑23 (MQ=255) gACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt > 1:636458/1‑76 (MQ=255) cAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggaca > 2:443299‑M2/1‑51 (MQ=255) cAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggaca < 2:416738‑M2/76‑26 (MQ=255) cAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggaca < 2:416727‑M2/76‑26 (MQ=255) attaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCa < 2:770051‑M2/27‑1 (MQ=255) ttaggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTNTACGATCTCAg < 1:154702‑M2/28‑1 (MQ=255) aggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTc > 1:488855‑M2/47‑76 (MQ=255) aggggctattccatttcatcgaccaaccaaancggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTc > 1:83964‑M2/47‑76 (MQ=255) ggggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCa < 1:466634‑M2/31‑1 (MQ=255) gggctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCaa > 1:672606‑M2/45‑76 (MQ=255) aaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaa < 2:678319‑M2/76‑31 (MQ=255) aaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaa < 2:678329‑M2/76‑31 (MQ=255) aaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaa > 1:234919‑M2/1‑46 (MQ=255) gctattccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAAt < 1:339557‑M2/34‑1 (MQ=255) ctctCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacac < 1:252560‑M2/76‑35 (MQ=255) ctctCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacac < 1:79123‑M2/76‑35 (MQ=255) tctcGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacg < 2:338875‑M2/76‑36 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa > 1:128095‑M2/40‑76 (MQ=255) attccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa < 1:1032259‑M2/37‑1 (MQ=255) attccacttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa > 1:166941‑M2/40‑76 (MQ=255) ttccatttcatcatacagcaaaaanggtgcagtacaatTTGTACGCTTTTTACTGTACNATTTCAGTCAAATCTaa < 2:899725‑M2/38‑1 (MQ=255) ctcGCGCTAAAAAAGGCNCCACTTTCGTGGAGCCTTTATTtgtactgacccnaaaatgatgaacagttaaacacga < 2:889867‑M2/76‑37 (MQ=255) cgcgCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacncgagg < 2:870010‑M2/76‑39 (MQ=255) cgcgCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgagg > 2:598424‑M2/1‑38 (MQ=255) ccatttcatcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAtt < 1:564840‑M2/40‑1 (MQ=255) cgcTAAAAAAGGCCCCACTTTCGTNGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggca < 2:186749‑M2/76‑41 (MQ=255) cTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcata < 2:337288‑M2/76‑43 (MQ=255) catcgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAg < 2:39724‑M2/46‑1 (MQ=255) aaaaGGCCCCACTTTCGTAGAGCCTTTATTtgttctgaccccaaaaagttggtcagttaaacacgaggcatatagg > 1:824824‑M2/1‑30 (MQ=255) cgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCACATCTAATTACATAGAtt > 1:431289‑M2/28‑76 (MQ=255) cgtccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGAtt > 2:656782‑M2/28‑76 (MQ=255) aaGGCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtc < 2:789458‑M2/76‑49 (MQ=255) ccaacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGCACGATNTCAGTCNAATCTAATTACATAGATTgan > 1:901724‑M2/25‑75 (MQ=255) gCCCCACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtcaga < 2:571071‑M2/76‑52 (MQ=255) caacaaaatgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGt < 2:530321‑M2/53‑1 (MQ=255) caacaaaatgggtgcagtacaatNTGTACGCNTNTTACTGCACGACTTCAGTCAAATCGACTTATATAGATTGAGt > 1:1003368‑M2/24‑76 (MQ=255) cccACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgatt > 2:1066548‑M2/1‑23 (MQ=255) cACTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattcc > 2:565722‑M2/1‑21 (MQ=255) aCTTTCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccg > 1:231506‑M2/1‑20 (MQ=255) atgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTa < 1:439237‑M2/60‑1 (MQ=255) ttCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgata > 1:434676‑M2/1‑17 (MQ=255) ttCGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgata > 1:434694‑M2/1‑17 (MQ=255) tgggtgcagtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATGGAGTGAAGGTAc < 2:162461‑M2/61‑1 (MQ=255) cGTGGAGCCTTTATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatatt < 1:331217‑M2/76‑62 (MQ=255) agtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaat > 1:619015‑M2/9‑76 (MQ=255) gtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCGAATCTAATTACATAGATTGAGTGAAGGTACGAGtaata < 1:764388‑M2/69‑1 (MQ=255) gtacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaata < 1:438274‑M2/69‑1 (MQ=255) gCCTTTATTtgtactgaccccaaaaagttggacngttaaacacgaggcatataggtctgattccgatgttcaattg > 1:870884‑M2/1‑9 (MQ=255) gCCTTTATTtgtactgaccccaaaaagttggacagttagacacgaggcatataggtctgattccgatattcaattg < 1:769104‑M2/76‑68 (MQ=255) tacaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaataa < 2:705070‑M2/70‑1 (MQ=255) acaatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTATATAGATTGAGTGAAGGTACGAGTAATAAc < 1:1063412‑M2/71‑1 (MQ=255) tttATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgctattcaattggag < 1:424805‑M2/76‑71 (MQ=255) tttATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggag < 1:30916‑M2/76‑71 (MQ=255) tttATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggag < 1:30926‑M2/76‑71 (MQ=255) caatTTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACg < 1:586318‑M2/72‑1 (MQ=255) tATTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtc > 2:117620‑M2/1‑4 (MQ=255) aTTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcn < 1:918496‑M2/76‑74 (MQ=255) aTTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtca < 1:606514‑M2/76‑74 (MQ=255) aTTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtca < 2:1033868‑M2/76‑74 (MQ=255) aTTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtca < 2:406430‑M2/76‑74 (MQ=255) aTTtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtca < 1:318744‑M2/76‑74 (MQ=255) aTTtgtaatgaccccaaaaagttggacagttaaacacgaggcacacaggtctgattccgatattcaattggagtca < 1:968017‑M2/76‑74 (MQ=255) ttGTACGCTTTTTACTATACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCct < 1:2920/76‑1 (MQ=255) ttgtactgagcccaaaaagtcagacagttaaacacgcggcatataggtctgcttccgatattcaattggagtcaga < 1:107772‑M2/76‑76 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga < 1:615029‑M2/76‑76 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga > 1:177370‑M2/1‑1 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga < 1:622009‑M2/76‑76 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga > 2:138445‑M2/1‑1 (MQ=255) ttgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcaga < 1:615048‑M2/76‑76 (MQ=255) tGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctg < 1:285165/76‑1 (MQ=255) | GTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTG > REL606/968253‑968403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |