Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,497,971	G→A	A22V (GCT→GTT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,497,971	0	G	A	100.0%	141.3 / NA	39	A22V (GCT→GTT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

TAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG  >  REL606/3497898‑3498042                                                                          |                                                                        tcaCGTGGCGTTCAGCCATTCANGTGTATTGGCNACGACCGGNTCCTTTCCAATCGTAAACATCANCTTGTAAACg                                                                       <  2:942501/74‑1 (MQ=255) tAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTACCAATCGTAAACATCAGCTTGTAAACg                                                                       <  1:98886/76‑1 (MQ=255)     gTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATag                                                                   <  1:497812/76‑1 (MQ=255)      ttGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATaga                                                                  <  2:119256/76‑1 (MQ=255)      ttGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCCTTCCANTCGTAANCATCAGCTTGTAAACGATagn                                                                  >  1:903342/1‑75 (MQ=255)         cGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTg                                                               >  2:561390/1‑76 (MQ=255)         cGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTg                                                               >  1:590934/1‑76 (MQ=255)                 cATTCATGTTTATTGGCCACGACCGGGTCCTTTCCACTCGTAAACATCAGCTTGTACACGATAGAGTGCTTAAggn                                                       >  1:924344/1‑75 (MQ=255)                    tCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCtt                                                    >  2:606116/1‑76 (MQ=255)                     cATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTc                                                   >  1:597260/1‑76 (MQ=255)                      aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCt                                                  <  2:102401/76‑1 (MQ=255)                       tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTa                                                 <  2:194514/76‑1 (MQ=255)                         tttATTGGCGACGACCGGGTCCTATCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACg                                               >  2:633318/1‑76 (MQ=255)                          ttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGCAAACGATAGAGTGCTTAAGGGCTTCTACGc                                              >  2:558053/1‑76 (MQ=255)                           tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGANAGAGTGCTTAAGGGCTTCTACGCt                                             <  2:177285/76‑1 (MQ=255)                                gCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCg                                        <  1:23905/76‑1 (MQ=255)                                 cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGt                                       >  2:478111/1‑76 (MQ=255)                                 cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGt                                       <  2:863077/76‑1 (MQ=255)                                  gacgacCGGGTCCTTTCNAACCGTAAACATCAGCTTGTAAACGATAGAGTGNGTAATGCCTGCGACGCTAAGCGTg                                      >  2:890016/1‑76 (MQ=255)                                     gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTAGGc                                   <  1:245262/76‑1 (MQ=255)                                       ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGa                                 >  1:411894/1‑76 (MQ=255)                                       ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGa                                 <  2:638504/76‑1 (MQ=255)                                        cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGAt                                >  2:795391/1‑76 (MQ=255)                                         gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATg                               <  2:333283/76‑1 (MQ=255)                                         gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATg                               <  2:817026/76‑1 (MQ=255)                                              cTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAatat                          <  1:666017/76‑1 (MQ=255)                                                  ccAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaa                      <  2:82572/76‑1 (MQ=255)                                                   cAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaaa                     >  2:38682/1‑76 (MQ=255)                                                      tCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACgg                  <  1:413308/76‑1 (MQ=255)                                                       cGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa                 <  1:623759/76‑1 (MQ=255)                                                         tAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGc               >  1:545266/1‑76 (MQ=255)                                                          aaaCATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa              >  2:467908/1‑76 (MQ=255)                                                          aaaCATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa              >  1:156598/1‑76 (MQ=255)                                                              aTCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa          >  1:629145/1‑76 (MQ=255)                                                              aTCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa          <  1:403166/76‑1 (MQ=255)                                                                 aGCTTGTAAACGATAGAGTGCTTANGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat       >  2:170518/1‑76 (MQ=255)                                                                 aGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat       <  1:175014/76‑1 (MQ=255)                                                                    ttGTAAANGATAGAGTGCTTAGTGNCTTCTACGCTAAGCGTNGGCGATGAGTATGTAAACGGAGCATTCATATAgg    <  2:955481/76‑1 (MQ=255)                                                                     tGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGc   <  1:281717/76‑1 (MQ=255)                                                                      gTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCg  >  1:272158/1‑76 (MQ=255)                                                                          |                                                                        TAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG  >  REL606/3497898‑3498042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑