| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,948,122 | C→T | intergenic (‑316/+370) | aslA ← / ← hemY | acrylsulfatase‑like enzyme/predicted protoheme IX synthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,948,122 | 0 | C | T | 98.3% | 213.4 / NA | 60 | intergenic (‑316/+370) | aslA/hemY | acrylsulfatase‑like enzyme/predicted protoheme IX synthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (38/19); minor base G (1/0); total (41/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGCAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTG > REL606/3948049‑3948194 | aaCGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAg < 2:804420/76‑1 (MQ=255) cGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGga < 1:819333/76‑1 (MQ=255) catttAATATATATGTTATTAATTGANTGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaa > 2:932489/4‑76 (MQ=255) cAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaa < 1:1045343/76‑1 (MQ=255) aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 1:495758/1‑76 (MQ=255) aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 1:281246/1‑76 (MQ=255) aaTTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGaaa > 2:647375/1‑76 (MQ=255) aaTTAATATATATGTTATTAATTGAATGAATTTTAGTATTCATTATCTATATGTATAGAATCGTGCGTAGgagata > 1:119759/1‑74 (MQ=255) ttAATATATATGTTATTAATTGAATGAATNTTATTATGCATTGTANATATGTGTAGTAGCGTGCGTAGGAGNAnta < 2:912369/76‑1 (MQ=255) atatatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTc > 1:672233/1‑76 (MQ=255) atatatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTc < 2:137707/76‑1 (MQ=255) atatatGTTATTAATTGAATGAATTTTACTATTCATTATATATATGTGTAGAATCGTGCGCGGGAGAAATATTCAc > 2:1051263/1‑76 (MQ=255) tatatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGAAGGAGAAATATTCACt > 2:441543/1‑76 (MQ=255) atatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTc > 1:620918/1‑76 (MQ=255) tatGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCa < 2:790731/76‑1 (MQ=255) atGTTATTAATTGAATGAATTTTATTNTTCATTCTATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACCCAg > 2:933676/1‑76 (MQ=255) atGTTATTAATTGAATGAATTTTATTNTTCATTCTATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACACAg > 2:933683/1‑76 (MQ=255) gTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGa < 1:573372/76‑1 (MQ=255) ttattaGTTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:158053/1‑76 (MQ=255) ttattaATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:580640/1‑76 (MQ=255) ttattaAATGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGaa > 1:709898/1‑76 (MQ=255) taATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGtta > 2:400124/1‑76 (MQ=255) ttGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGttatta > 1:230457/1‑76 (MQ=255) gaatgaatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACt > 1:477930/1‑76 (MQ=255) atgaatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCa < 2:580640/76‑1 (MQ=255) aatTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGa < 1:665571/76‑1 (MQ=255) atTTTATTATTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGaa > 2:64036/1‑76 (MQ=255) tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:487621/1‑76 (MQ=255) tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:487633/1‑76 (MQ=255) tattatTCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCaa > 1:324236/1‑76 (MQ=255) ttattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAag < 1:704192/76‑1 (MQ=255) tattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATCTTCACNCAGGAANTTATTACTCAGGAAGCAAagn > 1:907539/1‑75 (MQ=255) tattCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGNAAGTTATTACTCAGGAAGCAAaga < 2:188065/76‑1 (MQ=255) ttCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAgg > 2:356750/1‑76 (MQ=255) ttCATTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAgg > 2:356769/1‑76 (MQ=255) aTTATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGAtt > 2:1074324/1‑76 (MQ=255) ttATCTATATGTGTAGAATCGTGAGGAGTAGATNTATTCACTCAGGAAGTTATTACTNAGGAAGCAAAGAGGATTa > 2:109846/1‑76 (MQ=255) ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGTAGTTATTACTCAGGAAGCAAAGAGGATTa < 1:1054562/76‑1 (MQ=255) ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTa > 1:646802/1‑76 (MQ=255) ttATATATATGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTa > 1:31054/1‑76 (MQ=255) tatatataTGTGTAGAATCGTGCGTAGGAGAAATATTCGCTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTAc > 1:791324/1‑76 (MQ=255) tatatatGTGTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAg > 2:1009676/1‑76 (MQ=255) tatatGTGTAGAATCGTNCGTAGGAGAAATATTCACTCAGGAAGTTATTACNGAGGCAGCAGCGAGGATTACAGaa > 2:892971/1‑76 (MQ=255) tatGTGCAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAAtt > 1:165244/1‑76 (MQ=255) tgtgTAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAt > 2:782858/1‑76 (MQ=255) gtgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAGGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAtc > 1:578817/1‑76 (MQ=255) tgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTAtct < 1:802888/76‑1 (MQ=255) tgtAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAACCAAAGAGGATTACAGAATTAtct < 1:841955/76‑1 (MQ=255) tAGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCa > 1:648209/1‑76 (MQ=255) aGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCAt < 1:676033/76‑1 (MQ=255) aGAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCAt > 1:414898/1‑76 (MQ=255) gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa > 1:700342/1‑76 (MQ=255) gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa > 1:700321/1‑76 (MQ=255) gAATCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATa < 2:594045/76‑1 (MQ=255) aaTCGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATaa > 1:476980/1‑76 (MQ=255) cGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACaa > 2:591783/1‑76 (MQ=255) cGTGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACaa > 1:676748/1‑76 (MQ=255) tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:585269/76‑1 (MQ=255) tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:351109/76‑1 (MQ=255) tGCGTAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgt < 1:884405/76‑1 (MQ=255) gcgtAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAgtg < 2:457874/76‑1 (MQ=255) | AACGGCAATTAATATATATGTTATTAATTGAATGAATTTTATTATTCATTATATATATGTGTAGAATCGTGCGCAGGAGAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTG > REL606/3948049‑3948194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |