| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,519,066 | T→G | N192H (AAT→CAT) | yjhA ← | N‑acetylnuraminic acid outer membrane channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,519,066 | 0 | T | G | 90.7% | 160.5 / ‑0.5 | 44 | N192H (AAT→CAT) | yjhA | N‑acetylnuraminic acid outer membrane channel protein |
| Reads supporting (aligned to +/- strand): ref base T (0/4); new base G (26/13); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATTTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTATAGCGTCGTTTGCCATG > REL606/4518993‑4519137 | tGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCAtgtt < 1:683758/76‑1 (MQ=255)tGACGGTCAAGGTAGTCATATTCTATGTATGGCGNAATATCGGGCGTCATANGACATTGTAGAACAAANGCAtgtt > 2:953948/1‑76 (MQ=255) aCGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTcc < 1:298262/76‑1 (MQ=255) tCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCg > 1:714366/1‑76 (MQ=255) cAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGc > 1:377952/1‑76 (MQ=255) aaGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGcc > 1:807970/1‑76 (MQ=255) aGGTAGTCATATACTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGccc > 1:485787/1‑76 (MQ=255) cATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCtt > 1:842380/1‑76 (MQ=255) atatTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTa > 2:64475/1‑76 (MQ=255) atatTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTa > 1:195669/1‑76 (MQ=255) tatTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCCTTTCTTAt > 1:30707/1‑76 (MQ=255) atTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATg > 2:121968/1‑76 (MQ=255) atTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATg < 1:395831/76‑1 (MQ=255) atTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATg < 1:395834/76‑1 (MQ=255) tCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGt > 2:528808/1‑76 (MQ=255) tCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGt > 2:454015/1‑76 (MQ=255) tatgtatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATTTTCCGTCGGCCATTTCTTATGGttt < 2:1075735/76‑1 (MQ=255) tatgtatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGttt > 1:302414/1‑76 (MQ=255) tatgtatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGttt > 2:649232/1‑76 (MQ=255) atgtatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTg < 2:88731/76‑1 (MQ=255) atgtatgACGTAATATCGGGCGTCATATGGTATTGTAGAACGAATGCNTGTTCCNTCGCCCATTTCTTATGCtttn > 1:905437/1‑75 (MQ=255) tgtatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGc > 1:433926/1‑76 (MQ=255) tatgGCGTAATATCGGTCGTCATATGGTATTGTAGAACAAATGCATTTTCCGTCGCCCATTTCTTATGGTTTGCAt < 2:727243/76‑1 (MQ=255) tatgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCAt > 1:623374/1‑76 (MQ=255) tgGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAg < 1:1041000/76‑1 (MQ=255) ggCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATTTTCCGTCGCCCATTTCTTATGCTTTGCATAGc < 1:146192/76‑1 (MQ=255) gcgcAATATCGGGCGTCATATGGTAGTGTAGAACAAATGCATCTTCCGTCGCCCATTTCTTATGGTTTGCATAGCg < 2:924500/76‑1 (MQ=255) gTAATATCGGGCGTCATATGGTANTCTAGAANANATGCATCTTCCATCGCCCATTTCTGAAGGTTTGCATAGCGAt > 1:997847/1‑76 (MQ=255) tAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATa > 2:797532/1‑76 (MQ=255) tAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATa < 1:643885/76‑1 (MQ=255) tAATATCGGGCGTCATATGGTATTGTAGAACAAAGGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATa < 1:643881/76‑1 (MQ=255) aaTATCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATaa < 2:151560/76‑1 (MQ=255) atatCGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAAt > 2:801809/1‑76 (MQ=255) cGGGCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGtt > 1:525434/1‑76 (MQ=255) gggCGTCATATGGTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGtta > 2:1034466/1‑75 (MQ=255) tCATATGGTATTATAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGttt > 2:158220/1‑76 (MQ=255) cATATGGTATTGTAGAACTAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTg < 1:827786/76‑1 (MQ=255) cATATGGTATTGTAGAACAAATGCATTTTCCGTCGCCCATTTCTTATGCTTTGCATAGCGATAATCGTTCTGTTTg < 1:106364/76‑1 (MQ=255) cATATGGTATTGCAGAACCAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTg < 1:827773/76‑1 (MQ=255) gTATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGtat < 2:673412/76‑1 (MQ=255) tATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCTCTCTGTTTGCTGtata > 1:204808/1‑76 (MQ=255) tATTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGtata < 1:125984/76‑1 (MQ=255) aTTGTAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTGTAg > 1:156175/1‑76 (MQ=255) gTAGAACAAATGCATGTNCCGTCGCCCATTTCTTATGGTTTGCATAGCGATNATCGATGTGAGAGCTGTATAGcgt > 2:893073/1‑76 (MQ=255) tAGAACAAATGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTTTAGcgtc < 1:689844/76‑1 (MQ=255) cAAATGCATTTTCCGACGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTATAGCGTCGttca < 2:139069/76‑3 (MQ=255) aaTGCATGTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTATAGCGTCGTTTGcc > 1:206569/1‑76 (MQ=255) gCATGTTCCGTCGCCCATTTCTTATGGTTTGCATACCGATAATCGTTCTGTTTGCAGTATAGCGTCGTTTGCCATg > 1:811587/1‑76 (MQ=255) | TGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAGAACAAATGCATTTTCCGTCGCCCATTTCTTATGGTTTGCATAGCGATAATCGTTCTGTTTGCTGTATAGCGTCGTTTGCCATG > REL606/4518993‑4519137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |