Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	61.9 / NA	21	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851                                                                        |                                                                         cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  1:667707/1‑76 (MQ=255)  gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccga                                                                     <  2:100381/76‑3 (MQ=255)      cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCNGAAATCAGCCCCAATANCCGTAAAACGCCCATCNCCACCAg                                                                 >  2:960892/1‑76 (MQ=255)        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  1:634697/76‑1 (MQ=255)              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATNACAGCATAACGCCAATCCCCACCAGATAAAGCa                                                         >  1:871610/1‑76 (MQ=255)                 cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      >  2:782011/1‑76 (MQ=255)                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCAGTAt                                             >  2:673179/1‑76 (MQ=255)                            tgTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  2:291795/75‑1 (MQ=255)                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:259798/76‑1 (MQ=255)                             gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:52644/76‑1 (MQ=255)                             gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:776336/1‑76 (MQ=255)                              tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:808316/1‑76 (MQ=255)                                  gCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  1:260372/1‑76 (MQ=255)                                      tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  2:537887/1‑76 (MQ=255)                                          aaaTCAGCCCCAANAACAGCAAACNGCCAATCCCCACCANATAAAGCATCACGCCNGTATTANACAGGCTTTATAc                             >  2:927269/1‑76 (MQ=255)                                                gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  2:650120/76‑1 (MQ=255)                                                     aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:86526/76‑1 (MQ=255)                                                       tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:1031001/76‑1 (MQ=255)                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:117112/76‑1 (MQ=255)                                                              aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  1:559522/76‑1 (MQ=255)                                                                    ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  2:541271/76‑1 (MQ=255)                                                                    ccAATCCCCACCAGATAAAGCATGACGCCTGGATTAAACAGACTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:15906/1‑76 (MQ=255)                                                                     cAATCCACACCAGATAAANCATGACGCCTGTATTAAACAGGCNGTTTCCCGAGGCTGAATCNCGCACCAGCAGCca  <  2:108071/76‑1 (MQ=255)                                                                        |                                                                         CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑