| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,813 | C→T | intergenic (+144/‑51) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,813 | 0 | C | T | 94.1% | 116.5 / ‑4.0 | 34 | intergenic (+144/‑51) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (15/17); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCAC > REL606/3145742‑3145885 | taAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGt > 1:668436/1‑76 (MQ=255) aaGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTc > 1:68809/1‑76 (MQ=255) aaGTACGCCATCGTAATTATCGTTGGCGGTAAACAACCATTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTc > 1:761451/1‑76 (MQ=255) aGTACGCCCTCGTAACTATCGTTGGCGGTAGACATGCGTTGGATTTCAACGTTAACGGCTGATGGACATTGGGTCa < 1:970999/76‑1 (MQ=255) cTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCa > 2:503777/1‑76 (MQ=255) tAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAacac > 1:1035630/1‑76 (MQ=255) tATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAAc < 1:463151/76‑1 (MQ=255) aTCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAAcc > 1:323577/1‑76 (MQ=255) ttGGCGGTAAACAACCGTTAGATTTCAGCGTTAACGGCTGAAGANCATCGGGTCTATCGCCCAACACCAACCTCAt < 1:95132/76‑1 (MQ=255) gTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATa < 1:687061/76‑1 (MQ=255) tcaacagcCGTTGGATTTCAGCNTTGACGGCTGNAGGACATTNGGTCAATCGCGCAACACCTACCNCATGAAATaa < 2:939043/74‑1 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATaa < 2:42163/76‑1 (MQ=255) tAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATaa > 2:223826/1‑76 (MQ=255) aaaCAACAGTTGGATTTCAGCGTTAACGGCTGAAGGACATTCGGTCAATCGCCCAACACCAACATCATGAAATAAg > 2:966760/1‑76 (MQ=255) gTTGGATTTCTGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATa < 1:601890/76‑1 (MQ=255) gTTGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATa < 1:309353/76‑1 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATAc < 2:794284/76‑1 (MQ=255) ntGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGAttc < 1:923111/75‑3 (MQ=255) tGGATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATAcc > 2:1068148/1‑76 (MQ=255) ggATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCg > 1:472916/1‑76 (MQ=255) gATTTCAGCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGt < 2:790392/76‑1 (MQ=255) cggCGTTNAAGGCTGAAGGACATTGGGTCAATCGCACAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTAt > 1:948666/3‑76 (MQ=255) gCGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg > 2:437968/1‑76 (MQ=255) cGTTAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGa < 1:310453/76‑1 (MQ=255) tAACGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCa > 2:1001566/1‑76 (MQ=255) aaCGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTTTGGATACCGTCTTATGGAGCac < 2:1050937/76‑2 (MQ=255) aCGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 1:227883/76‑1 (MQ=255) aCGGCTGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 2:878755/76‑1 (MQ=255) tGAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCg > 1:670669/1‑76 (MQ=255) gAAGGACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGc < 2:611693/76‑1 (MQ=255) gACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 2:408841/76‑1 (MQ=255) gACATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 1:1035868/76‑1 (MQ=255) gACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 1:21439/76‑1 (MQ=255) cATTGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcac > 2:57211/1‑76 (MQ=255) | TAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCAC > REL606/3145742‑3145885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |