Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,249,887	C→T	M454I (ATG→ATA)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,249,887	0	C	T	100.0%	100.7 / NA	29	M454I (ATG→ATA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/18);  total (11/18)

TGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGT  >  REL606/3249816‑3249954                                                                        |                                                                    tGCGGCATCACACCGTCGTCGGCAGCAGCAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTg                                                                 <  1:628254/76‑1 (MQ=255)      cATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTa                                                            <  2:639814/76‑1 (MQ=255)      cATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTa                                                            <  1:596409/76‑1 (MQ=255)       aTCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTaa                                                           <  2:514707/76‑1 (MQ=255)       aTCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTaa                                                           <  1:720560/76‑1 (MQ=255)          ccacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg                                                        <  1:176341/75‑1 (MQ=255)          acacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg                                                        <  2:1028449/76‑1 (MQ=255)          acacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg                                                        >  1:886255/1‑76 (MQ=255)           cacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcgc                                                       <  1:127728/76‑1 (MQ=255)             ccGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAGGTAAAggcgc                                                     >  2:444534/1‑75 (MQ=255)             cagtcgtcGGCAGCAACAACCAGGACTACGATGTCCGTAGCCTGCGCACCACGCGCACGTATTGAAGTAAACgcgg                                                     >  1:795850/3‑76 (MQ=255)                 cgtcgGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTg                                                 <  2:377773/76‑1 (MQ=255)                        agcaACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCggg                                          <  2:74112/76‑1 (MQ=255)                         gcaACAACCACGACGCTGTTGTCAGTTGCCTGAGCACCACAAGCATGTATTGAAGTAAACGCGGCGTGCCCCgggg                                         <  2:446123/76‑1 (MQ=255)                           aacaacCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGgtg                                       <  1:465242/76‑1 (MQ=255)                              aacCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTcc                                    >  1:266253/1‑76 (MQ=255)                                    gACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTGCACGCGGCGTGCCCCGGGGTGTCCAGGAAg                              <  1:1064220/76‑1 (MQ=255)                                     aCTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAgg                             >  1:68193/1‑76 (MQ=255)                                            tGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCAt                      <  2:44620/76‑1 (MQ=255)                                             gTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATg                     >  1:821666/1‑76 (MQ=255)                                              tCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGc                    <  1:439982/76‑1 (MQ=255)                                                    gCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGtttt              <  1:1016798/76‑1 (MQ=255)                                                    gCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGtttt              >  1:463270/1‑76 (MQ=255)                                                     ccTGCGCNCCACGAGCACGTACTGNAGTAAACGCGGCGTGCNCCGGGGTGATCAGGAAGGTGATCATGCCGTTTTc             <  2:962265/76‑1 (MQ=255)                                                       tGCACACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCAGGGTGTCCAGGAAGNTGATCATGCCGTTTTCAg           >  1:844098/1‑76 (MQ=255)                                                         cgcACCACGAGCANGTATTGNAGTAATCGCGGCGTGNCCCGGGGTGTCCAGNGAGGTGATCANGCCGTTTTCAGtt         <  2:928508/76‑1 (MQ=255)                                                           caccacGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTc       >  2:308280/1‑76 (MQ=255)                                                            accacGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATAATGCCGTTTTCAGTTTCa      >  1:820812/1‑76 (MQ=255)                                                            acaacGAGCACGTATTGAAGTAAACGCGCCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCa      >  2:11002/4‑76 (MQ=255)                                                               acGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACg   >  2:1068408/1‑76 (MQ=255)                                                                cGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGt  <  1:533731/76‑1 (MQ=255)                                                                        |                                                                    TGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGT  >  REL606/3249816‑3249954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑