| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,035,629 | (C)5→6 | coding (228/768 nt) | wbbD ← | putative glycosyltransferase WbbD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,035,624 | 1 | . | C | 91.1% | 217.6 / 13.6 | 56 | coding (233/768 nt) | wbbD | putative glycosyltransferase WbbD |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (28/23); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
TTCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATA > REL606/2035550‑2035692 | ttCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑c > 1:1737544/1‑76 (MQ=255)ttCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑c < 1:1536907/76‑1 (MQ=255) tCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cc > 2:485960/1‑76 (MQ=255) aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 2:687372/1‑76 (MQ=255) aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 1:801814/1‑76 (MQ=255) aTTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑ccccc > 2:1799269/1‑76 (MQ=255) tttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccc < 2:4159/76‑2 (MQ=255) tttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccc < 2:217125/76‑2 (MQ=255) ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca < 2:1040859/76‑3 (MQ=255) ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca > 1:1368123/1‑74 (MQ=255) ttATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑cccccca < 2:681579/76‑3 (MQ=255) atcatcAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCCAaccc < 1:1603664/76‑1 (MQ=255) caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:1803884/1‑76 (MQ=255) caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:1145812/1‑76 (MQ=255) caGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCatat > 1:602909/1‑76 (MQ=255) aGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtt > 2:847485/1‑76 (MQ=255) aGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtt > 1:510911/1‑76 (MQ=255) aaTCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATAtttt > 1:1665062/1‑76 (MQ=255) gCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCa < 1:1038077/76‑1 (MQ=255) gCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCa < 2:492892/76‑1 (MQ=255) tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 1:1818635/1‑76 (MQ=255) tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 1:1881229/1‑76 (MQ=255) tATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAgtgt > 2:335678/1‑76 (MQ=255) aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg > 1:1410562/1‑76 (MQ=255) aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg > 1:1239991/1‑76 (MQ=255) aTTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGtgtg < 1:913835/76‑1 (MQ=255) tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGan < 1:938253/76‑2 (MQ=255) tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGan < 1:1385539/76‑2 (MQ=255) tACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGaa < 1:741643/76‑1 (MQ=255) cTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAAt < 2:1235257/76‑1 (MQ=255) tAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATc < 1:58237/76‑1 (MQ=255) aaaTCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCtt < 2:440764/76‑1 (MQ=255) tCATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCtttt < 1:363625/76‑1 (MQ=255) cATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTta > 1:649432/1‑76 (MQ=255) cATGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTNta < 2:1333582/76‑1 (MQ=255) aTGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtat < 1:750806/76‑1 (MQ=255) aTGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata > 1:1526854/1‑76 (MQ=255) tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata > 2:787300/1‑76 (MQ=255) tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata < 1:1713281/76‑1 (MQ=255) tGCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTtata < 1:1665601/76‑1 (MQ=255) gCATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTatat > 1:968744/1‑76 (MQ=255) cATACATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATAtt > 1:1709027/1‑76 (MQ=255) aCATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaa < 2:1818635/76‑1 (MQ=255) cATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaaa < 1:572351/76‑1 (MQ=255) cATTGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTaaa < 1:888879/76‑1 (MQ=255) tGATTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGCGTGAAATCTTTTATATTTAAatat > 1:1202868/1‑76 (MQ=255) aTTCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtct < 1:1817111/76‑1 (MQ=255) ttCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtctt > 2:1297013/1‑76 (MQ=255) ttCAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtctt < 2:1476726/76‑1 (MQ=255) cAACCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATAtcttct > 1:472493/1‑76 (MQ=255) aaCCCAAAATTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTtctc > 2:1497944/1‑76 (MQ=255) aaaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACt < 1:715620/76‑1 (MQ=255) aaaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACt < 1:1211926/76‑1 (MQ=255) aaaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTa > 1:387952/1‑76 (MQ=255) aaaTTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTAt > 2:1064778/1‑76 (MQ=255) aaTTTAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTAt < 1:805587/76‑1 (MQ=255) tttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCa > 2:1631112/1‑76 (MQ=255) tttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCa > 1:1075068/1‑76 (MQ=255) ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 1:1747270/1‑76 (MQ=255) ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 2:230617/1‑76 (MQ=255) ttAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCaa > 1:195345/1‑76 (MQ=255) tAACGCTGCCCCCCAACCCCATATTTTTTTCACGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt > 2:595991/1‑76 (MQ=255) tAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt > 1:1866278/1‑76 (MQ=255) tAACGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAAt < 1:94853/76‑1 (MQ=255) tAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa < 2:1589369/76‑1 (MQ=255) aaCGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa < 1:306904/76‑1 (MQ=255) aaCGCTGCCCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATa > 1:1211232/1‑76 (MQ=255) | TTCTATTTATATCATCAGAATCAGCACGTATTACTAAATCATGCATACATTGATTCAACCCAAAATTTAACGCTG‑CCCCCAACCCCATATTTTTTTCAAGTGTGAAATCTTTTATATTTAAATATCTTCTCCAACTATCAATA > REL606/2035550‑2035692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |