| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,106 | 0 | A | C | 25.8% | 151.6 / 15.5 | 62 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (39/7); new base C (0/16); total (39/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.97e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATG > REL606/1004033‑1004179 | cAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGacaac > 1:435474/1‑74 (MQ=255)cAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACAcc > 1:1710790/1‑76 (MQ=255)cAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACAcc > 2:689633/1‑76 (MQ=255)cAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACAcc < 2:1863651/76‑1 (MQ=255) agagCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCt > 1:1622330/1‑76 (MQ=255) agagCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCt > 1:1622329/1‑76 (MQ=255) gagCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTg > 2:370237/1‑76 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGc < 1:173317/76‑1 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGc < 1:1364406/76‑1 (MQ=255) aGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCAc > 2:643626/1‑76 (MQ=255) ggACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTg > 2:1778880/1‑76 (MQ=255) gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGc > 1:1319685/1‑76 (MQ=255) cGATCACCGCCAAAATCTTCCGTTCCATCATTATTTATAACCATTATGTTTTTTTTAATAACCCCTGCCACTGCCg < 2:1346234/76‑1 (MQ=255) gATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGt > 1:1565916/1‑76 (MQ=255) tCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCa > 1:1865372/1‑76 (MQ=255) cACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCaa > 2:1109838/1‑76 (MQ=255) ccTGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCAGGTTTTTTTTTATGACCCCTGCCACTGCCGTCAAt < 2:1479196/75‑1 (MQ=255) aCTGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAAt < 2:1614289/76‑1 (MQ=255) aCTGCAAAAATTTTGCGCTTCATCATTTTTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAAt < 2:1614299/76‑1 (MQ=255) tGCCAGAATTTTGCGCTTCATCATTTTTTTTTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATaa < 1:1701636/76‑1 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATaa < 1:741535/76‑1 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATaa > 2:1022649/1‑76 (MQ=255) gCCAGAATTTTGCCTTTCATCATTTTTTTTTCCCCTCATGTTTTTTTTTATGACACCTGCCACTGCCGTCAATAAg < 2:1388105/76‑1 (MQ=255) aGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTc > 1:485405/1‑76 (MQ=255) gAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCt > 2:1823176/1‑76 (MQ=255) gAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCt < 2:83581/76‑1 (MQ=255) aaTATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTg > 2:796350/1‑76 (MQ=255) tatTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTc > 1:1091848/1‑76 (MQ=255) atTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCa > 1:147415/1‑76 (MQ=255) ttGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:325173/1‑76 (MQ=255) ttGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:733390/1‑76 (MQ=255) ttGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCaa > 1:621281/1‑76 (MQ=255) gcgcTTCACCTTTATTTTTTCCCCTTATGTTTTTTTTTATAACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATa < 1:1762624/76‑1 (MQ=255) cgcTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATaa < 1:1472135/76‑1 (MQ=255) cgcTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaa < 1:1162280/76‑1 (MQ=255) gcTTCATCATTTTTTTTTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATaaa < 2:951574/76‑1 (MQ=255) gcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATaaa < 2:603912/76‑1 (MQ=255) cTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaa < 2:1091848/76‑1 (MQ=255) cTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaa > 1:479989/1‑76 (MQ=255) cTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaa > 1:1514116/1‑76 (MQ=255) ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaaa > 1:702723/1‑76 (MQ=255) catTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCACTAAAAATTTAc > 1:580516/1‑76 (MQ=255) attattTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACg > 1:1384811/1‑76 (MQ=255) ttttttattCCCCTGATGTTTTTTTTGAAGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACgg < 1:809980/73‑1 (MQ=255) ttatttatTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACgg < 1:1293979/76‑1 (MQ=255) atttattCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa < 1:1138784/76‑1 (MQ=255) atttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa > 1:1749331/1‑76 (MQ=255) atttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa > 1:1749313/1‑76 (MQ=255) atttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa > 1:1089706/1‑76 (MQ=255) ttattcCCCTCTTGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACt < 2:1543664/76‑1 (MQ=255) tattcCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTa < 1:1555665/76‑1 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt > 2:364638/1‑76 (MQ=255) taCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtg > 1:1775770/1‑76 (MQ=255) ccccTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga < 1:155621/75‑1 (MQ=255) ccTCATGTTTTTTTTTAGGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 2:1506877/76‑1 (MQ=255) ccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg > 1:503036/1‑76 (MQ=255) tCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgag < 2:734470/76‑1 (MQ=255) cATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgaga > 1:1777570/1‑76 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGagag > 1:627190/1‑76 (MQ=255) gtttttttttATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGtt < 2:1472311/74‑1 (MQ=255) ggTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGtt > 1:1484106/1‑76 (MQ=255) ttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt > 1:1335730/1‑76 (MQ=255) tttttATGCCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgtg < 1:1688843/76‑1 (MQ=255) ttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCt > 2:29316/1‑76 (MQ=255) ttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCt > 1:1285143/1‑76 (MQ=255) tATGACACCTGCCACTGCCGTCACTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCtt > 2:801440/1‑76 (MQ=255) aTGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 2:834504/1‑76 (MQ=255) gACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAt < 2:1438204/76‑1 (MQ=255) acacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAtg > 1:529132/1‑76 (MQ=255) | CAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATG > REL606/1004033‑1004179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |