Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,484,1050AG32.3% 53.0 / 13.0 31G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (3/18);  new base G (10/0);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 6.45e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA  >  REL606/1484033‑1484179
                                                                        |                                                                          
aCTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                         <  2:1054541/76‑1 (MQ=255)
  tgctgcTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttttn                                                                       <  1:237954/76‑2 (MQ=255)
    ctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                     <  1:44363/76‑1 (MQ=255)
     tgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATAc                                                                    <  1:1521855/76‑1 (MQ=255)
      gctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACt                                                                   >  1:1729660/1‑76 (MQ=255)
       ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAttt                                                                  >  2:1587997/1‑76 (MQ=255)
        ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                 <  1:817898/76‑1 (MQ=255)
        ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGTTGACACTGTTATGGCAGGGATTTTATACttt                                                                 <  1:781269/76‑1 (MQ=255)
        ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTAtttt                                                                 >  1:1429086/1‑76 (MQ=255)
               aCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAt                                                          <  2:1393379/76‑1 (MQ=255)
                cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                         <  1:10876/76‑1 (MQ=255)
                  aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTTTTTTTTCGAtttt                                                       >  1:689666/1‑75 (MQ=255)
                  aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTc                                                       <  2:1808795/76‑1 (MQ=255)
                        gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATg                                                 <  2:1158530/76‑1 (MQ=255)
                           tGGCATTGTCTATTTCGGCTCGCTGCTGACCCTGTTATGGCAGGGGCTTTTTATTTTTTTCGGCTTTCCCATGTCg                                              >  1:1609758/1‑76 (MQ=255)
                            ggCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCCTGTCgg                                             >  1:1539606/1‑76 (MQ=255)
                             gCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGt                                            <  1:1330650/76‑1 (MQ=255)
                               aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa                                          <  1:319605/76‑1 (MQ=255)
                                   tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTATTTTTTCGATTTTACCATGTCGGGAACGcc                                      >  1:253072/1‑76 (MQ=255)
                                    cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCg                                     <  1:805669/76‑1 (MQ=255)
                                       tttCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGaa                                  >  2:1574678/1‑76 (MQ=255)
                                         tCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTTACGATTTTACCCTGTCGGGAACGCCGGaaac                                >  1:72985/1‑74 (MQ=255)
                                         tCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACt                                <  1:1504901/76‑1 (MQ=255)
                                          cGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCCTGTCGGGAACGCCGGaaacg                               >  1:698951/1‑73 (MQ=255)
                                          cGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTATGATTTCTCCCTGTCGGGAACGCCCGAAATg                               >  1:1692748/1‑76 (MQ=255)
                                             cTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGGAACCCCGGAACTGACg                            >  2:415274/1‑76 (MQ=255)
                                               cGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACGGACGcc                          >  1:361121/1‑75 (MQ=255)
                                               cGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAAATTACGCt                          >  2:1719705/1‑76 (MQ=255)
                                               cGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt                          <  2:245619/76‑1 (MQ=255)
                                                         acTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATc                <  2:368504/76‑1 (MQ=255)
                                                            gTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt             <  1:74556/76‑1 (MQ=255)
                                                                  gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg       >  2:358444/1‑76 (MQ=255)
                                                                    aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt     >  1:1315419/1‑76 (MQ=255)
                                                                     gggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGttt    <  1:1865248/76‑1 (MQ=255)
                                                                       gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa  >  1:1850054/1‑76 (MQ=255)
                                                                        |                                                                          
ACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA  >  REL606/1484033‑1484179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑