| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,570,803 | Δ1 bp | coding (4/435 nt) | uspA → | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,570,802 | 0 | G | . | 98.4% | 314.0 / ‑0.9 | 64 | coding (3/435 nt) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (30/33); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
GACGCGGGTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTAT > REL606/3570727‑3570876 | gACGCGGGTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATg < 2:1090159/76‑1 (MQ=255)gACGCGGGTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATg > 1:1700476/1‑76 (MQ=255) ggTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTtata < 2:2103268/76‑1 (MQ=255) ggTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTtata < 1:37610/76‑1 (MQ=255) tGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATaaa < 1:1473831/76‑1 (MQ=255) gACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAac < 2:465779/76‑1 (MQ=255) aCGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAaca > 1:486949/1‑76 (MQ=255) cGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAaca < 1:1810562/76‑1 (MQ=255) cGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAacac > 2:54446/1‑76 (MQ=255) gTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAAcaca > 1:1489847/1‑76 (MQ=255) gTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAAcaca < 1:2094654/76‑1 (MQ=255) tAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACAt < 2:1156414/76‑1 (MQ=255) aaCGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACAtt < 2:59662/76‑1 (MQ=255) aaCGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACAtt > 2:1886364/1‑76 (MQ=255) cGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATtct < 2:377663/76‑1 (MQ=255) cGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATtct < 2:278037/76‑1 (MQ=255) aaaCGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGt > 1:1016913/1‑76 (MQ=255) aaCGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTc < 2:588379/76‑1 (MQ=255) gCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAt < 1:1980108/76‑1 (MQ=255) tAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtct > 1:1417090/1‑76 (MQ=255) tAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtct > 1:1350772/1‑76 (MQ=255) aGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctc > 1:121590/1‑76 (MQ=255) aGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctc < 1:61442/76‑1 (MQ=255) aGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctc > 1:1773911/1‑76 (MQ=255) aGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctc > 1:1364997/1‑76 (MQ=255) gCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctct < 1:604682/76‑1 (MQ=255) gCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctct < 2:195386/76‑1 (MQ=255) cTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctctc > 1:687447/1‑76 (MQ=255) cTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGAtctctc > 2:1719100/1‑76 (MQ=255) ggCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTccc < 2:549796/76‑1 (MQ=255) gCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTcccc < 2:1530059/76‑1 (MQ=255) gCCAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTcccc > 1:1881926/1‑76 (MQ=255) ccAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCg < 1:76317/76‑1 (MQ=255) ccAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCg < 1:927025/76‑1 (MQ=255) ccAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCg < 2:2050173/76‑1 (MQ=255) cAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGTGGTCGCTCTCTCCCCgg > 2:1425292/1‑76 (MQ=255) cAGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCgg > 2:1208063/1‑76 (MQ=255) aGTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGa < 2:1700476/76‑1 (MQ=255) gTCATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGaa < 1:911642/76‑1 (MQ=255) cATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAg < 2:1719499/76‑1 (MQ=255) cATCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAg < 2:548977/76‑1 (MQ=255) aTCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGc > 2:810187/1‑76 (MQ=255) aTCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGATCGATCTCTCCCCGGAAAGc < 1:627263/76‑1 (MQ=255) tCGACAACTTTATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCa < 1:1675563/76‑1 (MQ=255) tttATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTg > 1:635490/1‑76 (MQ=255) ttATGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTgg < 2:993290/76‑1 (MQ=255) aTGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTa > 1:745122/1‑76 (MQ=255) aTGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTa > 1:476235/1‑76 (MQ=255) tGGAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTag > 2:1143586/1‑76 (MQ=255) ggAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCGCCGGAAAGCAAAGTTCTGGTaga > 2:1873768/1‑76 (MQ=255) ggAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTaga > 1:2069477/1‑76 (MQ=255) ggAAGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTaga < 2:189329/76‑1 (MQ=255) aaGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAgaga < 1:1282562/76‑1 (MQ=255) aGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGaa < 1:530302/76‑1 (MQ=255) aGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGaa > 1:380534/1‑76 (MQ=255) aGGAGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGaa > 2:267730/1‑76 (MQ=255) aGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGc < 1:881903/76‑1 (MQ=255) aGTAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGc < 1:1246933/76‑1 (MQ=255) tAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAg < 2:629215/76‑1 (MQ=255) tAACACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAg > 2:1654510/1‑76 (MQ=255) aaCACTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGt > 1:1823129/1‑76 (MQ=255) acacTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGtc > 2:1691574/1‑76 (MQ=255) cacTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGtct < 2:1440876/76‑1 (MQ=255) cacTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGtct > 2:2050690/1‑76 (MQ=255) cacTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGtct > 2:801032/1‑76 (MQ=255) acTAT‑GCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGtctc > 2:382375/1‑76 (MQ=255) tGGCTTATAAACACACTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTAt < 2:1469155/76‑1 (MQ=255) | GACGCGGGTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAACTTTATGGAAGGAGTAACACTATGGCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTAT > REL606/3570727‑3570876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |