| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,997 | (A)6→7 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,991 | 1 | . | A | 98.3% | 231.7 / ‑2.6 | 59 | intergenic (‑192/‑131) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (29/29); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAT > REL606/3911916‑3912061 | gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt < 2:333006/76‑1 (MQ=255)gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt > 1:315239/1‑76 (MQ=255)gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt < 1:2087315/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑a < 1:1302911/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:1908056/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 1:365532/1‑76 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaa < 1:1743754/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaa < 2:1064986/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaac > 2:66514/1‑74 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAcaacaa < 2:1739631/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAAcaacaa > 2:1523211/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAt < 1:228411/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAtt < 1:1710520/76‑1 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAAttt < 1:1480558/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat < 2:622733/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat > 2:135730/1‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 2:1148646/1‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 1:1193086/1‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt > 1:685383/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg < 2:1280803/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg < 1:1146436/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTg > 1:578701/1‑76 (MQ=255) gggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGa > 2:560961/1‑76 (MQ=255) gggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGa > 1:997192/1‑76 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaa < 1:152163/76‑1 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaa < 1:128696/76‑1 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa < 2:1563267/76‑1 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa > 2:1704356/1‑76 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGaaa > 1:1509676/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 1:596272/76‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat > 2:1531200/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 1:1903041/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt < 1:920796/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAAttatta > 1:1837755/1‑76 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa < 1:411020/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1214293/1‑76 (MQ=255) tCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAAc > 2:1614573/1‑76 (MQ=255) cATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:331276/76‑1 (MQ=255) cTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatc > 2:707909/1‑76 (MQ=255) aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:732758/76‑1 (MQ=255) aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:2079423/76‑1 (MQ=255) aaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:2077201/76‑1 (MQ=255) aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:1696593/76‑1 (MQ=255) aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 2:2000432/1‑76 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATTATaaaa > 2:2121544/1‑76 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:1850672/76‑1 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:373415/76‑1 (MQ=255) tGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa < 1:1933114/76‑1 (MQ=255) aTTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCAaaaaaaatc < 2:1759761/76‑4 (MQ=255) tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc > 1:427710/1‑76 (MQ=255) tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc < 2:1349356/76‑1 (MQ=255) tttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc < 1:1416966/76‑1 (MQ=255) ttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCa > 2:119387/1‑76 (MQ=255) tttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaa > 1:567765/1‑76 (MQ=255) ttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa < 1:443702/76‑1 (MQ=255) ttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaa > 1:1800273/1‑76 (MQ=255) tcactcacTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 2:250035/76‑1 (MQ=255) tcactcacTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa > 2:86019/1‑76 (MQ=255) cactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAatat > 2:1709125/1‑76 (MQ=255) ctATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 1:1729068/1‑76 (MQ=255) tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 1:1080372/1‑76 (MQ=255) tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 1:991572/1‑76 (MQ=255) attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 1:1716929/1‑76 (MQ=255) attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg > 1:1312957/1‑76 (MQ=255) tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa < 2:1640893/76‑1 (MQ=255) tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa < 1:1670182/76‑1 (MQ=255) ttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:790330/1‑76 (MQ=255) ttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:352917/1‑76 (MQ=255) tattttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt < 1:1674387/73‑1 (MQ=255) attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 2:1808768/74‑1 (MQ=255) attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:503413/3‑76 (MQ=255) attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:373999/3‑76 (MQ=255) attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa > 1:1054877/3‑76 (MQ=255) attttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 2:977482/74‑1 (MQ=255) ttttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt > 2:1246920/2‑76 (MQ=255) ttttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt > 1:659705/2‑76 (MQ=255) | GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAT > REL606/3911916‑3912061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |