Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	2,298,585	+ACG	coding (498/1359 nt)	glpT ←	sn‑glycerol‑3‑phosphate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2298580	0 (0.000)	45 (0.700) +CGA	39/134	0.4	100%	coding (503/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter
	?	REL606	2298581 =	0 (0.000)					coding (502/1359 nt)	glpT	sn‑glycerol‑3‑phosphate transporter

GCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2298506‑2298580 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTAC  >  REL606/2298581‑2298655                                                                            |||                                                                            GCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACC                                                                               >  2:1756353/1‑76  CATATAGAGCGCCGCATGCCAGTCATTGAATCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCG                                                                              >  1:328074/1‑76   ATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGA                                                                             <  2:1313168/76‑1     ATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACG                                                                           <  2:935959/76‑1       AGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGAC                                                                         <  1:1290886/76‑1            GCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGT                                                                    >  2:1421990/1‑76            GCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGT                                                                    <  2:1454269/76‑1             CCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTG                                                                   >  2:1260096/1‑76             CCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTG                                                                   <  1:1976504/76‑1               GCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCG                                                                 <  2:1478397/76‑1                CATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGC                                                                >  2:2015513/1‑76                 ATGCCAGTCATTGACCCAGGCCACCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCA                                                               <  2:454926/76‑1                   GCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACA                                                             >  1:1504672/1‑76                     CAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGT                                                           <  1:1107272/76‑1                        TCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCC                                                        <  2:1428936/76‑1                         CATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCA                                                       >  1:690429/1‑76                         CATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCA                                                       >  2:2025711/1‑76                          ATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCAC                                                      <  1:1398741/76‑1                              AACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTG                                                  >  2:673212/1‑76                                CCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGAC                                                <  2:750962/76‑1                                  AGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACAC                                              >  2:2038254/1‑76                                     CCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAAT                                           <  1:1666383/76‑1                                       ATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGC                                         >  1:751250/1‑76                                             AGCAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCAC                                   >  2:289868/1‑76                                               CAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGc                                 >  1:166151/1‑75                                               CAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGT                                 <  2:734566/76‑1                                               CAGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGT                                 >  1:1125169/1‑76                                                AGGAACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTT                                >  1:1215201/1‑76                                                  GAACAGCAGCGGCGGAATACCACCACCGACGACGTTGCGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCT                              <  2:951933/76‑1                                                   AACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTT                             >  2:1521602/1‑76                                                    ACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTT                            <  2:211957/76‑1                                                    ACAGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTT                            <  2:430282/76‑1                                                      AGCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCT                          <  2:622719/76‑1                                                       GCAGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTG                         >  1:462816/1‑76                                                         AGCGGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCG                       <  1:1322467/76‑1                                                            GGCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACC                    >  1:1135904/1‑76                                                             GCGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCA                   <  2:1573642/76‑1                                                              CGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCAC                  <  1:211790/76‑1                                                              CGGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCAC                  <  1:1850239/76‑1                                                               GGAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACC                 >  1:832359/1‑76                                                                GAATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCA                >  2:889417/1‑76                                                                 AATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAG               <  2:665776/76‑1                                                                  ATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGT              >  1:1881733/1‑76                                                                  ATACCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGT              <  1:175928/76‑1                                                                     CCACCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCA           >  1:921653/1‑76                                                                       cCCACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACC         <  1:589276/75‑1                                                                          ACCGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATA      >  2:12512/1‑76                                                                            CGACGACGTTGTGCGCACAGTTCCACACTGCCACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGT    <  1:445563/76‑1                                                                            CGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGT    <  1:1773582/76‑1                                                                            CGACGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGT    <  1:1243590/76‑1                                                                              ACGGCGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTAC  <  1:2099288/76‑1                                                                            |||                                                                            GCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2298506‑2298580 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTAC  >  REL606/2298581‑2298655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.