Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2772854 =	NA (NA)	56 (0.870)	41/144	0.5	100%	noncoding (1/1343 nt)	IS186	repeat region
	?	REL606	= 4524527	0 (0.000)					coding (499/549 nt)	fimA	major type 1 subunit fimbrin (pilin)
*	?	REL606	= 2774196	NA (NA)	70 (1.080)	53/144	0.1	100%	noncoding (1343/1343 nt)	IS186	repeat region
	?	REL606	4524522 =	0 (0.000)					coding (494/549 nt)	fimA	major type 1 subunit fimbrin (pilin)

ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  REL606/4524522‑4524596                                                                                                                                                           nCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATG                                                                               >  1:1098503/2‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                              <  1:79869/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                              <  1:2033819/76‑1  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                              >  2:34979/1‑76  CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                              <  1:1385453/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             >  2:1233031/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             >  1:673552/1‑76   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  2:1884903/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  1:145386/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  2:86718/76‑1   GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  1:1198918/76‑1     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  2:1507360/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  2:1800692/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           <  1:855382/76‑1     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  1:1544944/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  2:234472/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  2:854773/1‑76     GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCC                                                                           >  1:1960904/1‑76      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                          >  2:1567668/1‑76      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                          <  1:805065/76‑1      AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                          <  1:164788/76‑1       GGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGC                                                                         <  1:246209/76‑1        GCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCA                                                                        <  1:551504/76‑1         CATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAA                                                                       >  2:310290/1‑76         CATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAA                                                                       <  1:878940/76‑1          ATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAAC                                                                      >  1:1945580/1‑76          ATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAAC                                                                      <  1:1468156/76‑1          ATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAAC                                                                      <  1:718572/76‑1           TCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACC                                                                     <  2:361508/76‑1            CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCC                                                                    >  2:424837/1‑76            CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCC                                                                    >  1:397826/1‑76            CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCC                                                                    >  2:1407506/1‑76            CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCC                                                                    >  2:769015/1‑76             GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                   <  2:767130/76‑1              TCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCG                                                                  >  1:1072600/1‑76                TTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGG                                                                >  1:1048600/1‑76                   ACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGC                                                             <  2:1754760/76‑1                   ACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGC                                                             >  2:1286195/1‑76                   ACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGCGC                                                             >  1:927147/1‑76                     GAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTG                                                           <  2:1311958/76‑1                       GGCCCCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCT                                                         <  1:898054/76‑1                       GGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCT                                                         <  1:1273188/76‑1                            CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                    <  1:1641900/76‑1                            CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                    >  2:1198081/1‑76                             GAGGCTTCACATACTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCG                                                   <  1:494741/76‑1                              AGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                                  <  2:428460/76‑1                                GCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGAT                                                <  1:1457838/76‑1                                 CGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATG                                               <  2:941747/76‑1                                 CGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATG                                               <  1:941884/76‑1                                  GTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATCGGCCGCAACCCCGGGTGCTGCTAATGCGGATGC                                              >  1:405560/1‑76                                    CGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGA                                            >  1:1594613/1‑76                                     GCATTCTTCAGATGGTTCGGCCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAC                                           <  1:687424/76‑1                                         TCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTC                                       <  2:1842434/76‑1                                            TCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    >  2:873837/1‑76                                              AGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGT                                  <  1:1527545/76‑1                                                ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                                >  1:1378331/1‑76                                                ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                                >  2:1751364/1‑76                                                 TGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCA                               <  1:1733238/76‑1                                                   GTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                             <  1:115674/76‑1                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           >  2:1835970/1‑76                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           <  2:926524/76‑1                                                     TCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTAT                           <  2:1286835/76‑1                                                      CAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATC                          <  2:1879016/76‑1                                                       AACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCA                         <  1:164244/76‑1                                                        ACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAA                        <  1:1620485/76‑1                                                          CCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATA                      <  2:674603/76‑1                                                            TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    >  2:1604910/1‑76                                                              AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                  <  1:432563/76‑1                                                              AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                  >  2:67506/1‑76                                                              AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                  <  1:944545/76‑1                                                               AGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                 <  1:480537/76‑1                                                               AGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTA                 <  2:2081309/76‑1                                                                 TTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACC               >  1:1159373/1‑76                                                                   AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA             <  2:2030208/76‑1                                                                    GCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAG            >  1:1398272/1‑76                                                                      GCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGT          <  1:1073985/76‑1                                                                       CTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT         <  1:30607/76‑1                                                                       CTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT         >  1:917081/1‑76                                                                        TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCCACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC        >  2:1753686/1‑76                                                                         TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA       <  1:2125485/76‑1                                                                          ATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAG      >  2:83663/1‑76                                                                            GGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  2:1963611/76‑1                                                                            GGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  2:1299465/76‑1                                                                              GCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  <  2:1276693/76‑1                                                                                                                                                           ACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774119‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  REL606/4524522‑4524596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.