| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 430,835 | C→T | intergenic (‑48/‑108) | insL‑2 ← / → lon | putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 430,835 | 0 | C | T | 98.7% | 297.0 / ‑3.7 | 77 | intergenic (‑48/‑108) | insL‑2/lon | putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (41/35); total (41/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
TCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCT > REL606/430771‑430906 | tCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGa < 1:191579/76‑1 (MQ=14) tGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAAc > 2:2111302/1‑76 (MQ=25) gTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATccc < 2:186429/76‑1 (MQ=255) gTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATccc > 2:484509/1‑76 (MQ=255) tAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATcccc > 2:1328145/1‑76 (MQ=255) aaTTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCa > 1:829701/1‑76 (MQ=255) aTTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCat > 2:1923093/1‑76 (MQ=255) ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata < 1:648333/76‑1 (MQ=255) ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata < 2:1956925/76‑1 (MQ=255) tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat < 1:1853475/76‑1 (MQ=255) tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat < 2:2045868/76‑1 (MQ=255) tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCatat > 1:1851241/1‑76 (MQ=255) cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata < 2:390032/76‑1 (MQ=255) cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata < 1:676077/76‑1 (MQ=255) cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata > 1:175060/1‑76 (MQ=255) cATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata < 1:1883029/76‑1 (MQ=255) aTCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc < 1:1538909/76‑1 (MQ=255) aTCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc < 1:1526971/76‑1 (MQ=255) cGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTg > 2:948548/1‑76 (MQ=255) ggCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGa < 1:247540/76‑1 (MQ=255) cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACg > 2:2084681/1‑76 (MQ=255) cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGAAAAAATCCCAATATACTGACg > 2:800105/1‑76 (MQ=17) cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa > 2:804984/1‑76 (MQ=255) cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa < 2:1159381/76‑1 (MQ=255) ttAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACa > 2:869632/1‑76 (MQ=255) ttAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACa < 1:1026854/76‑1 (MQ=255) tAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACAt > 1:1360021/1‑76 (MQ=255) tAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACAt > 2:226204/1‑76 (MQ=255) aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg > 1:1264859/1‑76 (MQ=255) aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg > 1:1488166/1‑76 (MQ=255) aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg < 1:990232/76‑1 (MQ=255) aaaTAATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATg > 2:1652960/1‑76 (MQ=255) aTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGtt < 1:365616/76‑1 (MQ=255) aTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGtt < 1:623271/76‑1 (MQ=255) aTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGtt < 2:782177/76‑1 (MQ=255) tCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTa < 2:130878/76‑1 (MQ=255) cATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTan < 1:442069/76‑2 (MQ=255) cATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTaa < 2:804392/76‑1 (MQ=255) cATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTaa > 1:1235458/1‑76 (MQ=255) atatCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAAt > 1:1058109/1‑76 (MQ=255) tatCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATa > 1:1739685/1‑76 (MQ=255) atCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAg < 1:1169965/76‑1 (MQ=255) tCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGCAACATCCCCATATACTGACGTACATGTTACTAGa > 1:535487/1‑76 (MQ=25) tCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGa < 1:1991646/76‑1 (MQ=255) cAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGAt < 1:749943/76‑1 (MQ=255) aGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATg < 1:1872718/76‑1 (MQ=255) ggCGTAATACCACAGCCCTTAAGTTAGTGCTTATGGGGAAACAGCCCCATATACTGACGTACATGTTAATAGATgg < 2:1164227/76‑1 (MQ=37) ggCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGCACATGTTAATAGATgg > 2:1820416/1‑76 (MQ=37) cGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCg > 2:2069247/1‑76 (MQ=255) gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGt > 2:238621/1‑76 (MQ=255) gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGt > 1:1956416/1‑76 (MQ=255) tAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTg > 2:870360/1‑76 (MQ=255) tAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGCTAATAGATGGCGTg > 2:237247/1‑76 (MQ=255) aaTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 2:146255/1‑76 (MQ=255) aaTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 1:1678526/1‑76 (MQ=255) tACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAg < 1:676720/76‑1 (MQ=255) tACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAg < 1:1715918/76‑1 (MQ=255) aCCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGc < 2:104624/76‑1 (MQ=255) ccACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGca < 1:809536/76‑1 (MQ=255) ccACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGca > 1:1388694/1‑76 (MQ=255) caACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAg > 2:1248245/1‑76 (MQ=255) aaCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGt < 1:2000242/76‑1 (MQ=255) aCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTc < 1:733008/76‑1 (MQ=255) aCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTc < 1:2033824/76‑1 (MQ=255) cccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCg > 1:1807243/1‑76 (MQ=255) cccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCg > 1:135576/1‑76 (MQ=255) cTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtg > 1:2011190/1‑76 (MQ=255) ttAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt > 1:66373/1‑76 (MQ=255) ttAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt < 2:615363/76‑1 (MQ=255) ttAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt > 1:1215603/1‑76 (MQ=255) ttAAGTTAGTGCTTATGGGGAAACAACCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt < 2:456825/76‑1 (MQ=255) tANGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTc > 2:1494164/1‑76 (MQ=255) tAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTc > 2:64766/1‑76 (MQ=255) aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa > 2:823519/1‑76 (MQ=255) aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa > 2:1912348/1‑76 (MQ=255) aGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCAt > 1:124766/1‑76 (MQ=255) aGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCAt < 2:229756/76‑1 (MQ=255) ttAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCt < 1:1370346/76‑1 (MQ=255) | TCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCT > REL606/430771‑430906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |