| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,718,725 | A→G | F161L (TTT→CTT) | ygaD ← | competence damage‑inducible protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,718,725 | 0 | A | G | 98.2% | 228.0 / ‑3.9 | 55 | F161L (TTT→CTT) | ygaD | competence damage‑inducible protein A |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (29/25); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
ATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAG > REL606/2718652‑2718795 | aTACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGtt < 1:1170549/76‑1 (MQ=255) aCCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgt > 1:1400604/1‑76 (MQ=255) aCCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgt > 2:1286017/1‑76 (MQ=255) aCCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgt > 2:1286029/1‑76 (MQ=255) ccGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAgttgtt < 1:1645188/76‑1 (MQ=255) cGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgttg < 2:1431497/76‑1 (MQ=255) cGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgttg > 2:428518/1‑76 (MQ=255) cGGATAGTCAATATGCTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGttgttg > 1:221781/1‑76 (MQ=255) ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGc < 2:1246909/76‑1 (MQ=255) aTAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCca > 2:719556/1‑76 (MQ=255) aTAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCca > 2:1465339/1‑76 (MQ=255) aGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCcaca > 2:820455/1‑76 (MQ=255) atatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTc > 1:851102/1‑76 (MQ=255) atGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTg > 2:723479/1‑76 (MQ=255) tCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAAt < 2:1978419/76‑1 (MQ=255) cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATg < 2:300246/76‑1 (MQ=255) tGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGc < 2:1980137/76‑1 (MQ=255) gAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATAc < 1:1618717/76‑1 (MQ=255) gAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATAc < 2:472119/76‑1 (MQ=255) aGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGc > 1:1555173/1‑76 (MQ=255) gCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCa > 1:1373772/1‑76 (MQ=255) cAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAg < 1:431352/76‑1 (MQ=255) cAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAg < 2:1290406/76‑1 (MQ=255) ttATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAg < 1:566604/76‑1 (MQ=255) tataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGc > 1:649923/1‑76 (MQ=255) ataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGcc > 2:758372/1‑76 (MQ=255) ataCTGTATGCTCATACAGTATCAAGTGCTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGcc > 1:1497594/1‑76 (MQ=255) taCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGccc > 1:584538/1‑75 (MQ=255) taCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCt < 1:1623566/76‑1 (MQ=255) aCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTg > 2:1223339/1‑76 (MQ=255) aCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTg < 1:551028/76‑1 (MQ=255) cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTga < 1:649879/76‑1 (MQ=255) cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTga > 2:1523042/1‑76 (MQ=255) cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTga < 2:1207309/76‑1 (MQ=255) tGTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgac < 1:483368/76‑1 (MQ=255) gTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacg > 1:432416/1‑76 (MQ=255) gTATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacg > 2:362854/1‑76 (MQ=255) tATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga < 1:837502/76‑1 (MQ=255) tATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga > 2:1704353/1‑76 (MQ=255) tATGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga > 2:2006604/1‑76 (MQ=255) aTGCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacgac > 1:2095683/1‑76 (MQ=255) gCTCATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGc > 2:952602/1‑76 (MQ=255) cATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAcc > 2:686244/1‑76 (MQ=255) cATACAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAcc > 2:1818476/1‑76 (MQ=255) ncAGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCa > 1:1451203/2‑76 (MQ=255) aGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATc < 2:2097087/76‑1 (MQ=255) aGTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATc < 2:378007/76‑1 (MQ=255) gTATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCa < 2:148227/76‑1 (MQ=255) tATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCAc < 1:1542385/76‑1 (MQ=255) tATCAAGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCAc < 2:1002687/76‑1 (MQ=255) tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCAc < 1:559091/76‑1 (MQ=255) aGTGTTTTGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcg < 2:1037088/76‑1 (MQ=255) ttGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTg > 1:300980/1‑76 (MQ=255) ttGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTg > 2:902498/1‑76 (MQ=255) tGTAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGa < 2:1660776/76‑1 (MQ=255) tAGAAGTTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAg < 1:514517/76‑1 (MQ=255) | ATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAG > REL606/2718652‑2718795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |