Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,161,106	A→G	E84G (GAA→GGA)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,161,106	0	A	G	100.0%	215.5 / NA	51	E84G (GAA→GGA)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

AGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGCGCG  >  REL606/4161034‑4161177                                                                         |                                                                        agagCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATg                                                                      >  1:217303/1‑76 (MQ=255) agagCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATg                                                                      >  2:1889799/1‑76 (MQ=255)  gagCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGt                                                                     <  1:1701533/76‑1 (MQ=255)  gagCGACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGt                                                                     <  1:2056337/76‑1 (MQ=255)   agCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTc                                                                    <  1:1208394/76‑1 (MQ=255)    gCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCa                                                                   <  2:1320680/76‑1 (MQ=255)     cTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCaa                                                                  >  1:343219/1‑76 (MQ=255)      tACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCaaa                                                                 <  2:267852/76‑1 (MQ=255)       aCAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAAt                                                                <  2:1978006/76‑1 (MQ=255)        cAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATc                                                               >  2:1325080/1‑76 (MQ=255)          gCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCg                                                             >  1:2082729/1‑76 (MQ=255)                aTTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGt                                                       >  1:645663/1‑76 (MQ=255)                 ttCCTAGCTGAAATTCGTCAGCTACCGTCTTGGCGAGCCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTg                                                      <  2:2032305/76‑1 (MQ=255)                 ttCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTg                                                      >  2:1817071/1‑76 (MQ=255)                   ccGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGAc                                                    <  1:1084329/76‑1 (MQ=255)                      aGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTa                                                 <  2:1342779/76‑1 (MQ=255)                         tGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTc                                              >  2:441540/1‑76 (MQ=255)                          gCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTcc                                             <  1:1817769/76‑1 (MQ=255)                           cAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCg                                            >  2:417978/1‑76 (MQ=255)                            aaTACGTCAGCTACCGCCTTGGCGAACCGGTGCTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGc                                           >  2:498387/1‑76 (MQ=255)                               aCGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCAcc                                        <  1:1009138/76‑1 (MQ=255)                                cGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCg                                       <  1:724541/76‑1 (MQ=255)                                 gTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGc                                      >  1:1144525/1‑76 (MQ=255)                                  tCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCt                                     >  1:5895/1‑76 (MQ=255)                                  tCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCt                                     >  1:1555621/1‑76 (MQ=255)                                  tCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGCGGCGTGACCTATTCCGCACCGCt                                     >  1:2061906/1‑76 (MQ=255)                                   cAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTg                                    >  2:709994/1‑76 (MQ=255)                                    aGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTgc                                   <  1:94883/76‑1 (MQ=255)                                    aGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTgc                                   <  2:1629977/76‑1 (MQ=255)                                    aGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTgc                                   >  2:1668891/1‑76 (MQ=255)                                       tACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGcgcg                                <  1:1275112/76‑1 (MQ=255)                                        aCCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt                               >  1:1276615/1‑76 (MQ=255)                                        aCCGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt                               >  2:957406/1‑76 (MQ=255)                                          cGCCTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTa                             >  1:855577/1‑76 (MQ=255)                                            ccgtGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTaaa                           <  1:176869/73‑1 (MQ=255)                                            ccTTGGCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTaaa                           <  2:1113880/76‑1 (MQ=255)                                                ggCGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGc                       <  2:14117/76‑1 (MQ=255)                                                  cGAACCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGt                     <  2:1384175/76‑1 (MQ=255)                                                    aaCCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCt                   >  1:1448329/1‑76 (MQ=255)                                                    aaCCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCt                   <  2:99555/76‑1 (MQ=255)                                                    aaCCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCt                   <  1:1668929/76‑1 (MQ=255)                                                     aCCGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTg                  <  1:1086809/76‑1 (MQ=255)                                                       cGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGt                <  1:1230182/76‑1 (MQ=255)                                                       cGGTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGCCTggg                >  2:241959/1‑75 (MQ=255)                                                        ggTGTTTGACGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTg               >  1:1017399/1‑76 (MQ=255)                                                                aCGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGa       >  2:994023/1‑76 (MQ=255)                                                                 cGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAg      >  1:1103020/1‑76 (MQ=255)                                                                 cGTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAg      <  2:999442/76‑1 (MQ=255)                                                                  gTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAgc     <  2:538101/76‑1 (MQ=255)                                                                  gTCCAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAgc     >  2:141369/1‑76 (MQ=255)                                                                     cAGGGATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGcgcg  <  1:1837562/76‑1 (MQ=255)                                                                         |                                                                        AGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGTCTGGTGATCTATGAGCGCG  >  REL606/4161034‑4161177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑