Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,437	C→T	G290E (GGG→GAG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,437	0	C	T	100.0%	156.3 / NA	41	G290E (GGG→GAG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/24);  total (17/24)

CAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCA  >  REL606/474363‑474509                                                                           |                                                                         cAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATccc                                                                         <  2:541569/76‑1 (MQ=255)  aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCg                                                                        >  2:1989465/1‑76 (MQ=255)  aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCg                                                                        >  1:1535605/1‑76 (MQ=255)  aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCg                                                                        <  1:1332985/76‑1 (MQ=255)   aaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGa                                                                       <  2:155710/76‑1 (MQ=255)     cAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACg                                                                     <  2:1220653/76‑1 (MQ=255)      aGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACgg                                                                    <  1:1494313/76‑1 (MQ=255)         ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGgaga                                                                 <  1:1475970/76‑1 (MQ=255)         ataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGgaga                                                                 >  1:1386839/1‑76 (MQ=255)              cGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACaaaa                                                            <  1:1318666/76‑1 (MQ=255)                 cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAAc                                                         <  2:1041198/76‑1 (MQ=255)                    cGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGc                                                      <  2:1360366/76‑1 (MQ=255)                          cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCa                                                <  1:1658959/76‑1 (MQ=255)                           aGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCaa                                               <  1:1271104/76‑1 (MQ=255)                            gCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGcnga                                              <  2:1490725/76‑4 (MQ=255)                              aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcg                                            <  1:1926668/76‑1 (MQ=255)                              aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcg                                            <  2:1535605/76‑1 (MQ=255)                              aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcg                                            <  1:258985/76‑1 (MQ=255)                              aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcg                                            <  1:33842/76‑1 (MQ=255)                              aCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcg                                            <  1:851777/76‑1 (MQ=255)                               cTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcgc                                           >  1:2105289/1‑76 (MQ=255)                               cTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAAcgc                                           >  1:918546/1‑76 (MQ=255)                                 gCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGt                                         <  1:1650864/76‑1 (MQ=255)                                  ccAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTc                                        <  2:1190250/76‑1 (MQ=255)                                   cAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTcg                                       <  1:1073096/76‑1 (MQ=255)                                    aGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTcgc                                      <  2:581345/76‑1 (MQ=255)                                      cGGTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCgcgc                                    >  2:768685/1‑76 (MQ=255)                                        gTTGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCaa                                  >  2:1664317/1‑76 (MQ=255)                                          tGCTGAATCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg                                >  1:1754664/1‑76 (MQ=255)                                               aaTCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGc                           >  1:346354/1‑76 (MQ=255)                                                 tCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGt                         <  2:227505/76‑1 (MQ=255)                                                 tCAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGt                         <  1:1896768/76‑1 (MQ=255)                                                  cAGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa                        >  2:51820/1‑76 (MQ=255)                                                   aGAATTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt                       >  1:1104720/1‑76 (MQ=255)                                                     aaTTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCg                     <  2:1197610/76‑1 (MQ=255)                                                      aTTAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGt                    >  1:991798/1‑76 (MQ=255)                                                       ttAACGGATCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg                   >  2:48977/1‑76 (MQ=255)                                                               tCAAACAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc           >  1:591860/1‑76 (MQ=255)                                                                 aaaCAACATCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACgg         >  2:948126/1‑76 (MQ=255)                                                                   acaacaTCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg       >  1:2086526/1‑76 (MQ=255)                                                                   acaacaTCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg       >  1:1661896/1‑76 (MQ=255)                                                                     aacaTCTCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGAc     >  2:314806/1‑76 (MQ=255)                                                                        aTCTCGACGGAGACAAAAAACAGCACCGCAAACGCGCCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCa  <  1:1638460/76‑1 (MQ=255)                                                                           |                                                                         CAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCA  >  REL606/474363‑474509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑