| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,610,902 | T→C | intergenic (‑42/+322) | cspI ← / ← ydfP | cold shock protein/conserved protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,610,902 | 0 | T | C | 96.0% | 406.8 / 0.1 | 99 | intergenic (‑42/+322) | cspI/ydfP | cold shock protein/conserved protein |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (36/59); total (39/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAAT > REL606/1610830‑1610974 | aTTTCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCg < 1:1349590/76‑1 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGa > 2:1714382/1‑76 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaa < 2:1621754/76‑1 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaa > 2:257714/1‑76 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaa < 1:2112612/76‑1 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaa < 2:891446/76‑1 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaa > 1:708618/1‑76 (MQ=255) aCTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaaa < 1:1654262/76‑1 (MQ=255) tAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaaaaa > 1:1031359/1‑76 (MQ=255) aaaCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCTCTTGGGCAGAATGGCCCGaaaaaaaa > 1:738136/1‑76 (MQ=255) aaaCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaaaaaa > 2:285789/1‑76 (MQ=255) aaaCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGaaaaaaaa > 2:1279119/1‑76 (MQ=255) aaCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAat < 1:1010469/76‑1 (MQ=255) aaCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAat < 1:488368/76‑1 (MQ=255) aCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAata > 2:1514923/1‑76 (MQ=255) aCCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAata < 1:1835157/76‑1 (MQ=255) ccAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAatat < 1:54488/76‑1 (MQ=255) cAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATc < 1:1534549/76‑1 (MQ=255) cAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATc < 1:916460/76‑1 (MQ=255) cAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATc < 1:424121/76‑1 (MQ=255) cAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATc > 1:1816237/1‑76 (MQ=255) aGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCa < 1:1601206/76‑1 (MQ=255) aGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCa > 1:1961733/1‑76 (MQ=255) aGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCa < 2:1518460/76‑1 (MQ=255) cATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCagag < 2:881357/76‑1 (MQ=255) cATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCagag > 1:1914078/1‑76 (MQ=255) cATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCagag < 1:1114406/76‑1 (MQ=255) cATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCagag < 2:1510676/76‑1 (MQ=255) cATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGCGCAGAATGGCCCGAAAAAAAATATCagag > 2:1256727/1‑76 (MQ=255) ttttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCagagag < 2:1193051/76‑1 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga < 2:1350995/76‑1 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga > 1:545297/1‑76 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga < 1:957301/76‑1 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga > 1:1088560/1‑76 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga > 1:1416700/1‑76 (MQ=255) tttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAgagaga > 1:1898543/1‑76 (MQ=255) ttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGaa > 2:751862/1‑76 (MQ=255) ttGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGaa < 2:391867/76‑1 (MQ=255) ttAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGaaaaa > 1:1049753/1‑76 (MQ=255) tAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAAc > 1:1115397/1‑76 (MQ=255) tAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAAc > 1:480209/1‑76 (MQ=255) aGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAAcc < 1:740022/76‑1 (MQ=255) gACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACca > 2:1214602/1‑76 (MQ=255) aCATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACcaa < 1:1227129/76‑1 (MQ=255) aCATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACcaa < 2:1955662/76‑1 (MQ=255) aCATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACcaa < 1:1227119/76‑1 (MQ=255) catcatTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACcaac < 1:720648/76‑1 (MQ=255) catTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAg < 1:702553/76‑1 (MQ=255) tattaCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGCGAAAAACCAACAAGGaa > 2:417609/1‑76 (MQ=255) tattaCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGaa < 1:773434/76‑1 (MQ=255) tattaCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGaa < 1:1751360/76‑1 (MQ=255) attaCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGaaa < 2:187538/76‑1 (MQ=255) ttaCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAt < 2:1410382/76‑1 (MQ=255) aCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAtct > 1:1191984/1‑76 (MQ=255) aCCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAtct > 2:25654/1‑76 (MQ=255) ccTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAtctc < 1:140920/76‑1 (MQ=255) cTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCa < 2:1168578/76‑1 (MQ=255) cTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCa < 1:616749/76‑1 (MQ=255) cTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCa < 2:1727342/76‑1 (MQ=255) ttttGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa < 2:766259/76‑1 (MQ=255) ttttGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa < 2:1400797/76‑1 (MQ=255) ttttGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa < 2:741631/76‑1 (MQ=255) tttGAAGAAGTTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAGCTCAag > 2:714048/1‑76 (MQ=255) tttGAAGAAGTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAag > 1:1286531/1‑76 (MQ=255) aagaagTTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGt < 1:1701272/76‑1 (MQ=255) aagaagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGt > 1:749001/1‑76 (MQ=255) aagaagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGt < 2:1775780/76‑1 (MQ=255) agaagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTa < 1:574371/76‑1 (MQ=255) agaagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTa < 2:1554925/76‑1 (MQ=255) aagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa < 1:497848/76‑1 (MQ=255) aagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa < 1:1180041/76‑1 (MQ=255) aagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa < 1:208460/76‑1 (MQ=255) aagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa < 1:1432604/76‑1 (MQ=255) aagTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa < 2:919661/76‑1 (MQ=255) gTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGaaaaaa > 1:822545/1‑76 (MQ=255) gTTAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACaaa < 1:958462/76‑1 (MQ=255) ttAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaat > 2:1004796/1‑76 (MQ=255) ttAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaat < 1:1872323/76‑1 (MQ=255) tAGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaata > 2:605043/1‑76 (MQ=255) aGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataa > 2:1775363/1‑76 (MQ=255) aGCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataa > 2:1344609/1‑76 (MQ=255) gCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat < 2:907364/76‑1 (MQ=255) gCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat < 2:326697/76‑1 (MQ=255) gCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat > 1:1342747/1‑76 (MQ=255) gCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat > 1:874193/1‑76 (MQ=255) gCCCTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat > 1:713979/1‑76 (MQ=255) cccTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAataata < 2:484130/76‑1 (MQ=255) ccTTGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAAtaataa < 2:2059054/76‑1 (MQ=255) tGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAAt > 2:926070/1‑76 (MQ=255) tGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAAt > 1:944218/1‑76 (MQ=255) tGGGCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAAt < 2:1232633/76‑1 (MQ=255) gggCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAAtt < 1:2002189/76‑1 (MQ=255) agCAGAATGGTCCGAAAAAAAA‑ATCCGAGAGAAAAACCAACAAGGAAATCTCAAGGGGTACAAATAATAAAATtat > 2:351751/2‑76 (MQ=255) gCAGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATtat < 2:471058/76‑1 (MQ=255) aGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATaa > 1:326453/1‑76 (MQ=255) aGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATaa < 2:770872/76‑1 (MQ=255) aGAATGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATaa < 1:1210085/76‑1 (MQ=255) aaTGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACa < 1:2098984/76‑1 (MQ=255) tGGCCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAAt < 2:1901687/76‑1 (MQ=255) | ATTTCACTAAACCAGTCATTTTGTTAGACATCATTATTACCTTTTGAAGAAGTTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAAT > REL606/1610830‑1610974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |