Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,228,513 Δ46 bp coding (12‑57/861 nt) yraL ← predicted methyltransferase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3228513 3228558 46 64 [0] [2] 66 yraL predicted methyltransferase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 32285120 (0.000)47 (0.840) 35/132 0.6 97.9% coding (58/861 nt) yraL predicted methyltransferase
?REL606 3228559 = 2 (0.040)coding (11/861 nt) yraL predicted methyltransferase

CCTCGGCGGCAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGCGTCGGTACAATGTAAAGCTGGCCCTGAGAATTATCCGCCGATTGGTGTTGTTTCATTGTGTCGTCCGTATTGCC  >  REL606/3228437‑3228587
                                                                           |                                                                           
ccTCGGCGGCAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGG                                                                             <  1:736297‑M1/76‑1 (MQ=255)
   cggcggCAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgt                                                                          >  1:987856‑M1/1‑73 (MQ=255)
    ggcggcAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgtt                                                                         <  1:1964874‑M1/76‑5 (MQ=255)
        gcAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgttt                                                                     >  1:432239‑M1/1‑68 (MQ=255)
        gcAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgttt                                                                     >  2:929467‑M1/1‑68 (MQ=255)
         cAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttc                                                                    <  2:1394199‑M1/76‑10 (MQ=255)
          aaTCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttca                                                                   >  1:385496‑M1/1‑66 (MQ=255)
             cAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattg                                                                >  1:1485582‑M1/1‑63 (MQ=255)
             cAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattg                                                                >  2:531384‑M1/1‑63 (MQ=255)
              aGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgt                                                               <  1:1617996‑M1/76‑15 (MQ=255)
                aTCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgt                                                             >  2:1476850‑M1/1‑60 (MQ=255)
                  cAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcg                                                           >  1:41393‑M1/1‑58 (MQ=255)
                     cGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtcc                                                        <  1:607282‑M1/76‑22 (MQ=255)
                      ggCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccg                                                       >  1:1171756‑M1/1‑54 (MQ=255)
                        ccTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgta                                                     <  1:611534‑M1/76‑25 (MQ=255)
                        ccTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgta                                                     <  1:1801706‑M1/76‑25 (MQ=255)
                         cTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtat                                                    >  2:160793‑M1/1‑51 (MQ=255)
                            tAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgc                                                 >  2:1201390‑M1/1‑48 (MQ=255)
                            tAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgc                                                 >  2:603626‑M1/1‑48 (MQ=255)
                            tAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgc                                                 >  2:962826‑M1/1‑48 (MQ=255)
                                aCCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgat                                             <  1:818790‑M1/76‑33 (MQ=255)
                                 ccTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatt                                            >  2:128866‑M1/1‑43 (MQ=255)
                                    ctAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaa                                         >  2:325555‑M1/1‑40 (MQ=255)
                                    ctAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaa                                         <  2:1283378‑M1/76‑37 (MQ=255)
                                      aaCGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaata                                       >  2:617280‑M1/1‑38 (MQ=255)
                                       acgcacgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaangt                                      <  2:1418461‑M1/76‑40 (MQ=255)
                                         gcacgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattg                                    <  1:1017831‑M1/76‑42 (MQ=255)
                                          cacgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattga                                   <  2:706829‑M1/76‑43 (MQ=255)
                                          cacgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattga                                   >  1:152398‑M1/1‑34 (MQ=255)
                                           acgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgag                                  >  2:231457‑M1/1‑33 (MQ=255)
                                           acgcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgag                                  <  1:877296‑M1/76‑44 (MQ=255)
                                             gcTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagca                                >  1:54710‑M1/1‑31 (MQ=255)
                                              cTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcat                               >  1:1750284‑M1/1‑30 (MQ=255)
                                              cTGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcat                               >  1:30310‑M1/1‑30 (MQ=255)
                                               tGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcatt                              >  1:62689‑M1/1‑29 (MQ=255)
                                               tGGGTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcatn                              <  1:1753061‑M1/76‑48 (MQ=255)
                                                  gTGATATCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcg                           >  2:552219‑M1/1‑26 (MQ=255)
                                                     atatCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaa                        <  1:1280042‑M1/76‑54 (MQ=255)
                                                     atatCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaa                        <  2:1374328‑M1/76‑54 (MQ=255)
                                                       atCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaa                      <  1:1128635‑M1/76‑56 (MQ=255)
                                                       atCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaa                      >  1:829208‑M1/1‑21 (MQ=255)
                                                        tCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaa                     <  1:1577048‑M1/76‑57 (MQ=255)
                                                        tCCGCCAGATTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaa                     <  1:1880472‑M1/76‑57 (MQ=255)
                                                                aTTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatca             <  2:1201391‑M1/76‑65 (MQ=255)
                                                                aTTGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatca             <  2:129992‑M1/76‑65 (MQ=255)
                                                                 ttGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcac            >  1:1212348‑M1/1‑11 (MQ=255)
                                                                 ttGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcac            >  2:701756‑M1/1‑11 (MQ=255)
                                                                  tGCCGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcact           >  1:1159217‑M1/1‑10 (MQ=255)
                                                                    ccGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactgg         <  1:198931‑M1/76‑69 (MQ=255)
                                                                    ccGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactgg         >  1:1765407‑M1/1‑8 (MQ=255)
                                                                     cGATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactgga        >  2:1055871‑M1/1‑7 (MQ=255)
                                                                      gATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggat       >  2:1299810‑M1/1‑6 (MQ=255)
                                                                      gATTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggat       <  2:263448‑M1/76‑71 (MQ=255)
                                                                       aTTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggata      >  2:1746904‑M1/1‑5 (MQ=255)
                                                                       aTTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggata      <  2:68037‑M1/76‑72 (MQ=255)
                                                                       aTTGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggata      <  1:694392‑M1/76‑72 (MQ=255)
                                                                         tGGtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggataca    <  1:929467‑M1/76‑74 (MQ=255)
                                                                          ggtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacat   >  1:150752‑M1/1‑2 (MQ=255)
                                                                          ggtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacat   <  1:1609887‑M1/76‑75 (MQ=255)
                                                                          ggtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacat   <  1:300183‑M1/76‑75 (MQ=255)
                                                                           gtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacatt  <  2:30310‑M1/76‑76 (MQ=255)
                                                                           gtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacatt  <  1:1281456‑M1/76‑76 (MQ=255)
                                                                           gtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacatt  >  2:919650‑M1/1‑1 (MQ=255)
                                                                           gtgttgtttcattgtgtcgtccgtattgccgatttaatattgagcattgcgtaaaaaaaatatcactggatacatt  >  1:393357‑M1/1‑1 (MQ=255)
                                                                           |                                                                           
CCTCGGCGGCAATCAGATCAACGGCCTGTAATACCTCTAACGCACGCTGGGTGATATCCGCCAGATTGCCGATTGGCGTCGGTACAATGTAAAGCTGGCCCTGAGAATTATCCGCCGATTGGTGTTGTTTCATTGTGTCGTCCGTATTGCC  >  REL606/3228437‑3228587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑