| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,166,065 | C→T | P369S (CCG→TCG) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,166,065 | 0 | C | T | 98.4% | 243.4 / ‑4.6 | 64 | P369S (CCG→TCG) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (36/27); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCA > REL606/4165994‑4166137 | aGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCgaa < 2:639047/76‑1 (MQ=255) gCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCgaag < 1:1488874/76‑1 (MQ=255) gCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCgaag < 2:1609827/76‑1 (MQ=255) gtgtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCgaagaa < 1:1217960/76‑1 (MQ=255) tgtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGaaa < 1:1372852/76‑1 (MQ=255) tgtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGaaa > 2:125423/1‑76 (MQ=255) gtTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGaaaa > 1:508733/1‑76 (MQ=255) ctactCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCa > 1:1799887/1‑76 (MQ=255) tactCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCAc > 2:1306944/1‑76 (MQ=255) ctCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTg > 2:1122826/1‑76 (MQ=255) ctCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTg > 1:572050/1‑76 (MQ=255) tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTgg < 1:1959979/76‑1 (MQ=255) tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTgg > 1:174763/1‑76 (MQ=255) cGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGt > 2:750788/1‑76 (MQ=255) cGNTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGt > 2:1422753/1‑76 (MQ=255) gTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGtt > 1:611435/1‑76 (MQ=255) gTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGtt > 2:135987/1‑76 (MQ=255) ttCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTc > 1:598870/1‑76 (MQ=255) ttCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTc > 2:335321/1‑76 (MQ=255) tCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCa > 1:1833462/1‑76 (MQ=255) tCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCa > 1:39327/1‑76 (MQ=255) tGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCaaa > 1:243273/1‑76 (MQ=255) gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAAc > 1:805506/1‑76 (MQ=255) cGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATc < 1:86900/76‑1 (MQ=255) gTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCt < 2:294937/76‑1 (MQ=255) aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTAc < 2:1304305/76‑1 (MQ=255) aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTAc < 1:450061/76‑1 (MQ=255) aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTAc < 1:1576932/76‑1 (MQ=255) ggTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACg < 1:363564/76‑1 (MQ=255) ggTCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACg < 1:750788/76‑1 (MQ=255) tCCATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGc > 1:1768316/1‑76 (MQ=255) ccATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCa > 1:1866600/1‑76 (MQ=255) ccATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCa < 2:1670073/76‑1 (MQ=255) cATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCaa > 1:495635/1‑76 (MQ=255) cATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCaa > 2:26633/1‑76 (MQ=255) cATACCTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCaa < 2:516178/76‑1 (MQ=255) ccTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTg < 1:1190789/76‑1 (MQ=255) ccTGCGTCTCCAACGGGGCGGTTTGACGAAGAAAATTGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTg < 2:1797215/76‑1 (MQ=255) cTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTgg > 1:164621/1‑76 (MQ=255) cTGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTgg < 2:1309394/76‑1 (MQ=255) tGCGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGa < 2:988384/76‑1 (MQ=255) gCGTCTGCATCAGTGCGGTCTTTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGaa < 2:912395/76‑1 (MQ=255) gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGaa > 1:1129356/1‑76 (MQ=255) cGTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAAc > 2:770503/1‑76 (MQ=255) cGTCTGCAACAGTGCGGTCCGCGGAAGAAAATGGGTCTGGTGCCGGTCAGAGCCTTCATCTCCGGCAAGCTGGAAc < 2:1224199/76‑1 (MQ=255) gTCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACt > 2:10306/1‑76 (MQ=255) tCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTg > 1:1439626/1‑76 (MQ=255) tCTGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTg < 2:1062957/76‑1 (MQ=255) tGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCg > 1:481475/1‑76 (MQ=255) tGCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCg < 2:1704480/76‑1 (MQ=255) gCATCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGt > 1:1222966/1‑76 (MQ=255) aTCAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTgg > 1:1543676/1‑76 (MQ=255) cAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTc < 1:1456593/76‑1 (MQ=255) cAGTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTc > 2:976132/1‑76 (MQ=255) gTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCtt < 2:753737/76‑1 (MQ=255) gTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCtt < 2:1918295/76‑1 (MQ=255) gTGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCtt > 1:1334881/1‑76 (MQ=255) tGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACTGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTg > 1:1288949/1‑76 (MQ=255) tGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTg > 1:430135/1‑76 (MQ=255) tGCGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTg > 1:430116/1‑76 (MQ=255) cGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCt > 2:1715112/1‑76 (MQ=255) cGGTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTC‑TCCACGGCAAGCTGGAACTGCGTGGTCTTGGTa > 1:1864036/1‑76 (MQ=255) ggTCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTa < 2:2015224/76‑1 (MQ=255) tCTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTacc > 2:962056/1‑76 (MQ=255) cTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTacca < 1:810302/76‑1 (MQ=255) cTGTCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTacca < 1:212686/76‑1 (MQ=255) | AGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCA > REL606/4165994‑4166137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |