| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,286 | A→C | L7R (CTT→CGT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,286 | 0 | A | C | 98.4% | 254.5 / ‑3.4 | 62 | L7R (CTT→CGT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (45/16); total (45/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAA > REL606/475213‑475358 | agaAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGc > 1:480477/1‑76 (MQ=255)agaAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGGGGCGAGAAGc > 2:262062/1‑76 (MQ=255) gaAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAcgcg < 2:111172/76‑1 (MQ=255) aaaTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgg > 1:1345464/1‑76 (MQ=255) aaaTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgg > 2:859715/1‑76 (MQ=255) aaaTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgg > 2:1090903/1‑76 (MQ=255) aaaTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgg > 1:1962042/1‑76 (MQ=255) aaTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCggg > 1:1842372/1‑76 (MQ=255) aTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgggg < 2:964705/76‑1 (MQ=255) aTACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCgggg > 2:802486/1‑76 (MQ=255) aCGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTg < 2:402680/76‑1 (MQ=255) tAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCg < 1:1994757/76‑1 (MQ=255) aGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCgg > 2:1319178/1‑75 (MQ=255) gTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTg > 1:1988047/1‑76 (MQ=255) tttATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGa < 1:364933/76‑1 (MQ=255) tttATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGa > 1:1953207/1‑76 (MQ=255) aTTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGc > 2:354091/1‑76 (MQ=255) aTTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGc > 1:949568/1‑76 (MQ=255) ttGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCa > 1:1938843/1‑76 (MQ=255) ggCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCAtt < 1:458750/76‑1 (MQ=255) ggCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCAtt > 2:1506394/1‑76 (MQ=255) ggCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCAtt > 2:877138/1‑76 (MQ=255) gCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTc > 2:581348/1‑76 (MQ=255) ccAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTcc > 1:1810745/1‑76 (MQ=255) ccAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTcc > 2:840746/1‑76 (MQ=255) ccAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTcc > 1:1391274/1‑76 (MQ=255) cGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCttt < 2:1113612/76‑1 (MQ=255) gAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCtttt > 2:1894683/1‑76 (MQ=255) gAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCtttt < 1:2015494/76‑1 (MQ=255) gAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGAGGCGTGATGCATTCTGGCTCCtttt > 1:481201/1‑76 (MQ=255) gATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCt < 1:405836/76‑1 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtg > 2:1888750/1‑76 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtg > 1:1754625/1‑76 (MQ=255) aTAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtg < 2:1480007/76‑1 (MQ=255) aanGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtggt > 2:1425096/1‑76 (MQ=255) acngCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtggtg > 2:1423407/4‑76 (MQ=255) aaGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtggtg > 2:135965/1‑76 (MQ=255) aGGCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtggtgg > 2:243427/1‑76 (MQ=255) ggCGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTggtggt > 2:795578/1‑76 (MQ=255) cGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTa < 2:293067/76‑1 (MQ=255) cGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTa > 2:1724704/1‑76 (MQ=255) cGAGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTa > 2:1012835/1‑76 (MQ=255) aGCACAAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGttnct < 2:1426681/76‑4 (MQ=255) cacaAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGt > 2:492497/1‑76 (MQ=255) cacaAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGt > 2:1897892/1‑76 (MQ=255) caAGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTcc > 2:1477501/1‑76 (MQ=255) aaGGCCGCCAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCa > 2:288268/1‑76 (MQ=255) gccgccAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttt > 1:1549912/1‑76 (MQ=255) gccgccAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttt > 1:241666/1‑76 (MQ=255) ccAACAATGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTgg > 1:718776/1‑76 (MQ=255) caacaaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGc > 1:1517140/1‑76 (MQ=255) aacaaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGcc < 2:457318/76‑1 (MQ=255) acaaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCg > 2:1765159/1‑76 (MQ=255) aaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCggg > 1:889365/1‑76 (MQ=255) aaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCggg > 1:87700/1‑76 (MQ=255) aaTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCggg > 1:852073/1‑76 (MQ=255) aTGGTGGTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGa < 2:651774/76‑1 (MQ=255) tggtggTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaa > 2:167558/1‑76 (MQ=255) tggtggTGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaa < 1:435957/76‑1 (MQ=255) ggtggtGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaaa < 1:1125506/76‑1 (MQ=255) ggtggtGATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaaa < 2:1217199/76‑1 (MQ=255) gtggtgATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaaaa > 1:82246/1‑76 (MQ=255) ggtgATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAAcc < 2:610911/76‑1 (MQ=255) tgATACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaa > 2:471101/1‑76 (MQ=255) aTACGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaaaa > 2:543422/1‑76 (MQ=255) | AGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAA > REL606/475213‑475358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |