Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	650,345	A→C	F153V (TTT→GTT)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	650,345	0	A	C	100.0%	295.8 / NA	69	F153V (TTT→GTT)	mrdA	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/39);  total (30/39)

TGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAA  >  REL606/650276‑650417                                                                      |                                                                         tGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTa                                                                    <  2:279820/76‑1 (MQ=255)  gACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTAc                                                                   >  1:1746154/1‑76 (MQ=255)   aCGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACt                                                                  <  2:1140778/76‑1 (MQ=255)   aCGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACt                                                                  <  2:1416034/76‑1 (MQ=255)    cGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTg                                                                 >  1:1266481/1‑76 (MQ=255)     gTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGa                                                                >  1:1099708/1‑76 (MQ=255)     gTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGa                                                                >  1:1577705/1‑76 (MQ=255)     gTGGGTCAACGCCGAACCGTAAGGATAGCAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGa                                                                >  2:1436603/1‑76 (MQ=255)       gggTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGAtt                                                              >  1:1182802/1‑76 (MQ=255)       gggTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGAtt                                                              <  2:1741866/76‑1 (MQ=255)       gggTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACGTCAACACCCGGAACACGGTACTGAtt                                                              <  2:1088692/76‑1 (MQ=255)             aCGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGc                                                        <  1:72432/76‑1 (MQ=255)              cGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCa                                                       >  2:1112179/1‑76 (MQ=255)                 cGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAg                                                    >  1:1554620/1‑76 (MQ=255)                   aaCCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCg                                                  <  2:302125/76‑1 (MQ=255)                      cGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGc                                               >  2:1928584/1‑76 (MQ=255)                       gTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGangt                                              <  2:1421893/76‑4 (MQ=255)                        tAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTa                                             >  2:306795/1‑76 (MQ=255)                        tAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTa                                             <  2:1074523/76‑1 (MQ=255)                         aaGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTAc                                            >  2:1907679/1‑76 (MQ=255)                             aTAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGt                                        <  2:1955855/76‑1 (MQ=255)                             aTAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGt                                        <  2:591348/76‑1 (MQ=255)                             aTAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGt                                        >  1:959368/1‑76 (MQ=255)                              tAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTa                                       <  2:247938/76‑1 (MQ=255)                              tAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTa                                       <  2:1871149/76‑1 (MQ=255)                              tAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTa                                       >  2:984627/1‑76 (MQ=255)                              aaGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTa                                       <  2:1243757/75‑1 (MQ=255)                                gTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACt                                     <  1:1590779/76‑1 (MQ=255)                                 tAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACtt                                    >  1:1480020/1‑76 (MQ=255)                                 tAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACtt                                    >  1:188498/1‑76 (MQ=255)                                 tAACGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACtt                                    <  2:625281/76‑1 (MQ=255)                                  aaCGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTc                                   >  1:46980/1‑76 (MQ=255)                                  aaCGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTc                                   <  2:980253/76‑1 (MQ=255)                                  aaCGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTc                                   >  2:964485/1‑76 (MQ=255)                                  aaCGACGTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTc                                   >  2:124896/1‑76 (MQ=255)                                   acgacgTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCg                                  <  1:1772241/76‑1 (MQ=255)                                      acgTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTc                               <  1:1943737/76‑1 (MQ=255)                                       cgTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCa                              >  1:1269150/1‑76 (MQ=255)                                        gTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAg                             <  1:1372782/76‑1 (MQ=255)                                        gTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAg                             >  1:1867459/1‑76 (MQ=255)                                        gTTTATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAg                             <  2:668550/76‑1 (MQ=255)                                         tttATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAgg                            <  1:1889345/76‑1 (MQ=255)                                          ttATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGt                           >  2:1219070/1‑76 (MQ=255)                                          ttATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGt                           >  2:1741089/1‑76 (MQ=255)                                          ttATAGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGt                           <  1:968860/76‑1 (MQ=255)                                           tataGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGtt                          >  1:1809671/1‑76 (MQ=255)                                            ataGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTa                         <  2:603828/76‑1 (MQ=255)                                            ataGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTa                         >  1:1561548/1‑76 (MQ=255)                                             taGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAg                        >  2:72702/1‑76 (MQ=255)                                             taGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAg                        <  1:229693/76‑1 (MQ=255)                                              aGCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGt                       <  2:572763/76‑1 (MQ=255)                                               gCCTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGtt                      <  1:49331/76‑1 (MQ=255)                                                ccTTTAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGttt                     <  1:1290606/76‑1 (MQ=255)                                                  tttAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTc                   <  2:198083/76‑1 (MQ=255)                                                  tttAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTc                   <  2:1210987/76‑1 (MQ=255)                                                   ttAACTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCa                  <  2:1221772/76‑1 (MQ=255)                                                     aaCTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCAcc                <  1:740355/76‑1 (MQ=255)                                                     aaCTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCAcc                >  1:1604641/1‑76 (MQ=255)                                                      aCTTCAACACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCg               >  1:1943868/1‑76 (MQ=255)                                                           aaCACCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATa          >  2:860988/1‑76 (MQ=255)                                                             cacCCGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATaga        <  2:1679220/76‑1 (MQ=255)                                                               cccGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAgg      <  2:281580/76‑1 (MQ=255)                                                               cccGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAgg      <  1:1836136/76‑1 (MQ=255)                                                                ccGGAACGCGATACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGt     <  1:941132/76‑1 (MQ=255)                                                                 cGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTg    >  1:141879/1‑76 (MQ=255)                                                                 cGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTg    <  2:947442/76‑1 (MQ=255)                                                                 cGGAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTg    <  1:36807/76‑1 (MQ=255)                                                                  ggAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGa   <  2:1146492/76‑1 (MQ=255)                                                                  ggAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGa   <  2:1141537/76‑1 (MQ=255)                                                                   gAACACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGaa  >  2:1319496/1‑76 (MQ=255)                                                                      |                                                                         TGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCAACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAA  >  REL606/650276‑650417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑