| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,186 | T→A | I374F (ATC→TTC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,186 | 0 | T | A | 97.6% | 131.3 / NA | 42 | I374F (ATC→TTC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (17/23); minor base G (0/1); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGG > REL606/474113‑474256 | tCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACg < 1:1389946/76‑1 (MQ=255) cGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCt < 2:766637/76‑1 (MQ=255) gTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTg < 2:634895/76‑1 (MQ=255) ttCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGcc < 1:274989/76‑1 (MQ=255) ggTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGt < 2:1141142/76‑1 (MQ=255) ggTCTCCGCCAGATATTTCCCCAGTAGTGCAAACAGTACGGGGTTGAGCATAATCGACAGGACCGCCCCTGCCAGt < 2:1395704/76‑1 (MQ=255) tCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTAc < 1:1715727/76‑1 (MQ=255) ttCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCa > 1:1138496/1‑76 (MQ=255) cAGATATTCCGCAAGTAGTACGACAGGTACAGGGTTGAGCATAACGAAAAGGAACGCCCCTGCCAGTACCAGGttt < 2:557697/76‑1 (MQ=255) gATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTg < 2:244930/76‑1 (MQ=255) tttCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCg < 1:56671/76‑1 (MQ=255) ttCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCgg < 2:1351759/76‑1 (MQ=255) tctcCAGTGGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGc < 1:1007671/76‑1 (MQ=255) ctcCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGcc < 1:1306100/76‑1 (MQ=255) ccAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTg > 1:1533838/1‑76 (MQ=255) ccAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTg < 2:1519068/76‑1 (MQ=255) agtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCg > 1:1191635/1‑76 (MQ=255) agtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCg > 1:424091/1‑76 (MQ=255) agtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCg > 1:572860/1‑76 (MQ=255) gtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCgg > 1:166908/1‑76 (MQ=255) gtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCgg < 2:777159/76‑1 (MQ=255) tggtGCGAACAGTCCCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc < 1:456364/74‑1 (MQ=255) tagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc > 2:462269/1‑76 (MQ=255) tagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc > 1:1531418/1‑76 (MQ=255) taggGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc < 2:166302/76‑1 (MQ=255) tGCGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAga > 1:229723/1‑75 (MQ=255) gCGAACAGTCCCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTa < 2:650963/76‑1 (MQ=255) cGAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCAAGTACCAGGTTTTGTCCGCCCTGCGCCAGTaa > 2:189319/1‑76 (MQ=255) gAACAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTaaa < 1:1487325/76‑1 (MQ=255) aCAGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAAtt < 2:296373/76‑1 (MQ=255) aGTACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGGAAATTCa > 2:1229161/1‑76 (MQ=255) tgccGGGTTGAGCATAATCGACAGGAGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAAt < 1:556104/74‑1 (MQ=255) tACCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAAt < 1:420079/76‑1 (MQ=255) cccGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATg < 2:802250/75‑1 (MQ=255) aCCGGGTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTATCCGGCCTGCGGCAGTAAATTCAATg > 1:796998/1‑76 (MQ=255) ccGGGTTGAGCATAATCGACAGAAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGc < 2:1659392/76‑1 (MQ=255) gTTGAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCAtt > 2:1203902/1‑76 (MQ=255) gAGCATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTccc < 2:1283831/76‑1 (MQ=255) cATAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGt > 1:861686/1‑76 (MQ=255) aTAATCGACGGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTc > 1:1336885/1‑76 (MQ=255) aTAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTc < 2:374538/76‑1 (MQ=255) tAATCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTcc > 2:1483529/1‑76 (MQ=255) tCGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGc < 1:1127264/76‑1 (MQ=255) cGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGcc < 2:229723/76‑1 (MQ=255) cGACAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGcc < 1:364723/76‑1 (MQ=255) cAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTGCCGCCAgg > 2:1505561/1‑76 (MQ=255) cAGGAACGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAgg < 1:1299651/76‑1 (MQ=255) | TCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGG > REL606/474113‑474256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |