| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,103,979 | T→A | intergenic (‑40/+116) | cytR ← / ← priA | DNA‑binding transcriptional dual regulator/primosome assembly protein PriA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,103,979 | 0 | T | A | 98.4% | 239.7 / ‑2.7 | 63 | intergenic (‑40/+116) | cytR/priA | DNA‑binding transcriptional dual regulator/primosome assembly protein PriA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (31/31); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01 | |||||||||||
TCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGT > REL606/4103909‑4104049 | tCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTa < 2:25339/76‑1 (MQ=255) cgcgGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTAc < 1:661117/76‑1 (MQ=255) cgGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACtt > 2:525417/1‑76 (MQ=255) gCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTca < 1:1233837/76‑1 (MQ=255) gCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTca > 2:826622/1‑76 (MQ=255) gCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTca < 1:820330/76‑1 (MQ=255) cAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTcac > 1:685643/1‑76 (MQ=255) cAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTcac > 1:773338/1‑76 (MQ=255) aGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTcaca > 2:630869/1‑76 (MQ=255) gTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACAt < 2:267472/76‑1 (MQ=255) ttCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCg > 1:86867/1‑76 (MQ=255) cgngCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCTAGTTTAATTCACATCGGc > 2:1212404/4‑76 (MQ=255) ccTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGc > 2:1682586/1‑76 (MQ=255) cangCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGc > 2:1212391/4‑76 (MQ=255) cTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCa > 2:1428730/1‑76 (MQ=255) cttcttCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACa < 2:106631/76‑1 (MQ=255) tcttcGCTGTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACAtt < 2:938697/76‑1 (MQ=255) cttcGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACAttt > 1:957326/1‑76 (MQ=255) cttcGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGCTTACTTCACATCGGCAACAttt > 2:312664/1‑76 (MQ=255) tcGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACAttttt < 2:1640532/76‑1 (MQ=255) tcGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACAttttt > 1:1183022/1‑76 (MQ=255) tcGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACAttttt > 1:194183/1‑76 (MQ=255) cGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTa < 1:1212270/76‑1 (MQ=255) gCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAg < 2:52846/76‑1 (MQ=255) gCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAg > 1:752490/1‑76 (MQ=255) cTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGc < 1:312665/76‑1 (MQ=255) ttCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAg > 2:770635/1‑76 (MQ=255) cacaCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGAt < 1:206374/76‑1 (MQ=255) cacaCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGAt < 2:806073/76‑1 (MQ=255) ccactcactCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGAtn < 1:1576822/75‑2 (MQ=255) acacTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATa < 2:1181436/76‑1 (MQ=255) acacTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATa < 1:475264/76‑1 (MQ=255) acacTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATa > 1:1630786/1‑76 (MQ=255) acacTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATa > 1:29839/1‑76 (MQ=255) actcactcCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAgc > 2:1127757/1‑76 (MQ=255) actcactcCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAgc > 2:526768/1‑76 (MQ=255) ctcactcCTCGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAgcg < 1:226192/76‑1 (MQ=255) ctcctcGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTg < 2:1063819/76‑1 (MQ=255) ctcGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGaaa < 1:606948/76‑1 (MQ=255) tcGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGaaaa < 2:43254/76‑1 (MQ=255) cGCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAAc > 2:368897/1‑76 (MQ=255) gCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACg < 2:1065169/76‑1 (MQ=255) gCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACg < 1:1105362/76‑1 (MQ=255) gCCTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACg < 1:261081/76‑1 (MQ=255) cTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGt > 2:1102715/1‑76 (MQ=255) cTGGCACGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGt > 1:81228/1‑76 (MQ=255) aCGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAg > 1:900306/1‑76 (MQ=255) aCGTCAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAg < 2:339537/76‑1 (MQ=255) cAGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAAtt > 2:820568/1‑76 (MQ=255) aGGCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAAttt < 1:720283/76‑1 (MQ=255) ggCGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAAtttt < 1:349524/76‑1 (MQ=255) cGTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCa > 1:856014/1‑76 (MQ=255) gTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTAc > 1:192669/1‑65 (MQ=255) gTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTAc < 2:192669/65‑1 (MQ=255) gTACTACATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCAt > 2:31248/1‑76 (MQ=255) tactacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATg < 1:1026636/76‑1 (MQ=255) tactacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATg < 1:526769/76‑1 (MQ=255) tactacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATg > 1:390850/1‑76 (MQ=255) tactacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATg > 1:197398/1‑76 (MQ=255) tactacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATg > 1:848283/1‑76 (MQ=255) actacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGa < 2:1497571/76‑1 (MQ=255) tacATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGaaa < 2:1347809/76‑1 (MQ=255) cATCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAg < 2:507387/76‑1 (MQ=255) aTCCAAGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAgt < 2:1259396/76‑1 (MQ=255) | TCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGT > REL606/4103909‑4104049 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |