| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,096 | G→A | P155L (CCG→CTG) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,096 | 0 | G | A | 96.2% | 85.6 / ‑3.7 | 26 | P155L (CCG→CTG) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (19/6); total (20/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAAT > REL606/4202025‑4202160 | gcagcaGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCaa < 1:382956/76‑1 (MQ=255)gcagcaGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCaa < 2:339963/76‑1 (MQ=255) cagcagCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAAt > 1:213174/1‑76 (MQ=255) cagcagCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAAt > 2:385857/1‑76 (MQ=255) cagcTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTa < 2:560507/76‑1 (MQ=255) cACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATa > 1:253438/1‑76 (MQ=255) aCCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAg > 1:117514/1‑76 (MQ=255) ccTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGCTAgg > 2:707860/1‑76 (MQ=255) ccTGTTCTTCGCTCAGGTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGGGCATCAGCAATTTACCGCCGATAgg < 2:1122427/76‑1 (MQ=255) gTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGgcgc > 1:689626/1‑76 (MQ=255) gTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGgcgc > 2:624454/1‑76 (MQ=255) tcttcGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGGGCATCAGCAATTTACCGCCGATAGGCGCgg < 2:1200811/76‑1 (MQ=255) cttcGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGa > 1:1539404/1‑76 (MQ=255) tcGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACa > 2:907534/1‑76 (MQ=255) gtggggCAAAAAAGGCTTTCCCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCAt < 2:563653/73‑1 (MQ=255) ggCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGa > 2:883901/1‑76 (MQ=255) tAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGc > 2:1426075/1‑76 (MQ=255) aaaGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTa > 2:591519/1‑76 (MQ=255) aaGGCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTac > 1:459984/1‑76 (MQ=255) gCTTTACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACt > 2:1374831/1‑76 (MQ=255) tttACCTGCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGt > 2:727235/1‑76 (MQ=255) tGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACAATCGCATCAGATGCCGCCACCGCACCTgg > 2:149674/1‑76 (MQ=255) cgCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTgg < 2:117514/75‑1 (MQ=255) gCACCGGAAGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGt > 2:618795/1‑76 (MQ=255) gCACCGGAAGCGTGCATCAGCAATTTAACGCCGATAGGCGCGGACATTCGCATCAGATGCGTACCCTGTACCTGGt > 2:405548/1‑76 (MQ=255) cGGAAGCGTGCATCAGCAATTTCCCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGat < 2:689625/76‑1 (MQ=255) aaGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGataat > 2:1065523/1‑76 (MQ=255) aaGCGTGCATCAGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTAGTCGataaa > 2:890098/1‑75 (MQ=255) | GCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAAT > REL606/4202025‑4202160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |