Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,360,241	G→A	V635I (GTT→ATT)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,360,241	0	G	A	100.0%	160.7 / NA	43	V635I (GTT→ATT)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/15);  total (28/15)

TCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACA  >  REL606/2360168‑2360313                                                                          |                                                                         tCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACAtt                                                                        <  2:761335/76‑1 (MQ=255)   tGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGc                                                                      <  2:518445/76‑1 (MQ=255)     gcgcAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGa                                                                    >  1:1686550/1‑76 (MQ=255)        cAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAAt                                                                 >  1:874360/1‑76 (MQ=255)         aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATc                                                                >  2:1237718/1‑76 (MQ=255)         aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATc                                                                <  1:433582/76‑1 (MQ=255)            aaaaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCaa                                                             <  1:1135336/76‑1 (MQ=255)             aaaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCaaa                                                            >  2:1657540/1‑76 (MQ=255)              aaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAg                                                           >  2:226584/1‑76 (MQ=255)               aaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAgc                                                          <  2:1585347/76‑1 (MQ=255)                   tCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCg                                                      >  2:767777/1‑76 (MQ=255)                    cctgacctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGa                                                     >  2:68472/1‑76 (MQ=255)                      tgacctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAAc                                                   <  2:290124/76‑1 (MQ=255)                         cctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAActct                                                >  2:1623789/1‑76 (MQ=255)                           tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc                                              >  2:928983/1‑76 (MQ=255)                           tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc                                              <  2:199519/76‑1 (MQ=255)                           tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc                                              >  2:1509390/1‑76 (MQ=255)                            gatgatCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCg                                             >  1:778447/1‑76 (MQ=255)                               gatCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGtt                                          <  2:908169/76‑1 (MQ=255)                                atCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTATCCGGCTg                                         >  2:1051711/1‑76 (MQ=255)                                 tCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGc                                        >  2:1411297/1‑76 (MQ=255)                                  cGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCa                                       >  1:402234/1‑76 (MQ=255)                                     cGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGt                                    >  1:1521285/1‑76 (MQ=255)                                      ggTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCACGTc                                   >  1:1295289/1‑76 (MQ=255)                                        tCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcgc                                 <  2:591425/76‑1 (MQ=255)                                            cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa                             >  2:1238136/1‑76 (MQ=255)                                            cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa                             >  1:736949/1‑76 (MQ=255)                                            cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa                             >  1:540769/1‑76 (MQ=255)                                              gCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTAcc                           >  2:1016672/1‑76 (MQ=255)                                               cAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGATGCAGGTCGCGCTACCg                          >  2:337769/1‑76 (MQ=255)                                               cAGTACGACGCTACGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCg                          >  1:665991/1‑76 (MQ=255)                                                  tACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCgtgt                       >  1:153382/1‑76 (MQ=255)                                                   acgacgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCgtaaa                      <  2:827404/76‑4 (MQ=255)                                                    cgacgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTc                     <  2:841277/76‑1 (MQ=255)                                                      acgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCAt                   <  1:1232917/76‑1 (MQ=255)                                                      acgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCAt                   <  1:1004811/76‑1 (MQ=255)                                                        gCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCt                 >  1:1254363/1‑76 (MQ=255)                                                         cTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCtt                >  1:618598/1‑76 (MQ=255)                                                                aaTGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAt         >  2:434832/1‑76 (MQ=255)                                                                   ggCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTg      <  2:1660878/76‑1 (MQ=255)                                                                   ggCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTg      <  1:1652983/76‑1 (MQ=255)                                                                    gCTGACATTGGTAAATCCAAACCGCCGATCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGa     <  2:965389/76‑1 (MQ=255)                                                                     cTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGaa    >  1:1006657/1‑76 (MQ=255)                                                                       gACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAaca  >  2:1339362/1‑76 (MQ=255)                                                                       gACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAaca  >  1:1118336/1‑76 (MQ=255)                                                                          |                                                                         TCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACA  >  REL606/2360168‑2360313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑