| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,897,276 | C→T | Q21* (CAA→TAA) | yqeA → | predicted amino acid kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,897,276 | 0 | C | T | 96.7% | 213.1 / NA | 62 | Q21* (CAA→TAA) | yqeA | predicted amino acid kinase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (38/21); total (41/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATCGT > REL606/2897203‑2897346 | tgagtgagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTaa < 2:1286035/76‑1 (MQ=255)tgagtgagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTaa > 1:999984/1‑76 (MQ=255) gtgagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATg < 1:1559528/76‑1 (MQ=255) tgagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGa > 1:1035851/1‑76 (MQ=255) gagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGaa < 2:380777/76‑1 (MQ=255) gagGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGaa > 1:68260/1‑76 (MQ=255) agGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGaaa < 2:561163/76‑1 (MQ=255) aaaaaTAAAAAAAAAAATTCTGCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCg < 1:1218855/76‑1 (MQ=255) aaaTGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGt < 2:323292/76‑1 (MQ=255) aaaTGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGt > 2:1201159/1‑76 (MQ=255) aaTGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTa > 2:944835/1‑76 (MQ=255) aTGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaa > 2:330010/1‑76 (MQ=255) tGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaa < 2:375619/76‑1 (MQ=255) tGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaa < 2:1499671/76‑1 (MQ=255) tGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaa < 1:336660/76‑1 (MQ=255) tGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaa > 2:637826/1‑76 (MQ=255) tGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGGaaa > 2:693983/1‑76 (MQ=255) gAGTAAGAAAGTTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaaa < 1:1272328/76‑1 (MQ=255) aGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTaaaaa < 2:729083/76‑1 (MQ=255) gTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAAt > 2:119585/1‑76 (MQ=255) aaGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTa > 2:268689/1‑76 (MQ=255) aaaTTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTtc > 2:1702181/1‑76 (MQ=255) ttGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCa > 2:635295/1‑76 (MQ=255) ttGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCa > 2:1441864/1‑76 (MQ=255) tGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAg > 2:892582/1‑76 (MQ=255) gTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAgg > 1:1694754/1‑76 (MQ=255) gTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAgg > 1:1448728/1‑76 (MQ=255) ttCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGc > 1:1246570/1‑76 (MQ=255) tctcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCa > 2:1436733/1‑76 (MQ=255) tctcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTAAAAATTACTTCTCAGGCa > 1:723018/1‑76 (MQ=255) ctcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCaa > 2:166562/1‑76 (MQ=255) ctcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCaa > 2:715723/1‑76 (MQ=255) ctcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGGAAATGGAAGCGGTAAAAATTACTTCTCAGGCaa > 2:283092/1‑76 (MQ=255) tcGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAAt > 2:1235611/1‑76 (MQ=255) cGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAAtt > 2:1141707/1‑76 (MQ=255) ccTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAAttgtt > 2:1323388/1‑76 (MQ=255) tGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGa < 1:56590/76‑1 (MQ=255) gCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGAttt > 1:822996/1‑76 (MQ=255) gCGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGAttt > 1:768110/1‑76 (MQ=255) cGGGACTGCGCTGGGCGCTGACCTGGCCGGGTAAATGAAAGGGGTAAAACTTACTTCTCCGGAAATTGTTGATTTa > 2:129406/1‑76 (MQ=255) cGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTa < 2:454637/76‑1 (MQ=255) cGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTa > 2:129397/1‑76 (MQ=255) cGGGAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTa < 2:663765/76‑1 (MQ=255) gggAATGCGCTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTaa < 2:534044/76‑1 (MQ=255) gcTGGGCGATGACCTGGCCGGGTAAATGACAGCGGTAAAAATTACTTCTAAGGCACTTGTTGATTCAATTGCCAAg > 2:583645/1‑76 (MQ=255) gcTGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCCCAGGCAATTGTTGATTTAATTGCCCAg > 2:664623/1‑76 (MQ=255) tGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAggg < 2:295188/76‑1 (MQ=255) tGGGCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAggg > 2:1281729/1‑76 (MQ=255) gggCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGa > 1:767405/1‑76 (MQ=255) gggCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGa > 1:834379/1‑76 (MQ=255) gggCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGa > 2:2682/1‑76 (MQ=255) gCGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACa < 2:957042/76‑1 (MQ=255) cGATGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACAt > 2:1316887/1‑76 (MQ=255) aTGACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGa > 2:889416/1‑76 (MQ=255) gACCTGGCCGGGTAAATGAAAGCGGTACAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAg > 2:634527/1‑76 (MQ=255) gACCTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAg > 2:204904/1‑76 (MQ=255) cTGGCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTa < 2:1448727/76‑1 (MQ=255) ggCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTTCCCAGGGACATGAAGTTATc < 2:1363149/76‑1 (MQ=255) ggCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATc > 1:1545729/1‑76 (MQ=255) gCCGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATCg < 2:370821/76‑1 (MQ=255) ccGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATCGt > 1:727864/1‑76 (MQ=255) ccGGGTAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATCGt < 2:1135395/76‑1 (MQ=255) | TGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTAAAAATTACTTCTCAGGCAATTGTTGATTTAATTGCCCAGGGACATGAAGTTATCGT > REL606/2897203‑2897346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |