Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,193,684	A→G	intergenic (‑116/‑41)	yjaB ← / → metA	predicted acetyltransferase/homoserine O‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,193,684	0	A	G	100.0%	377.0 / NA	86	intergenic (‑116/‑41)	yjaB/metA	predicted acetyltransferase/homoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/40);  total (46/40)

TCCTTGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGC  >  REL606/4193611‑4193756                                                                          |                                                                         tCCTTGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTg                                                                        >  1:1022171/1‑76 (MQ=255) tCCTTGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTg                                                                        >  1:825460/1‑76 (MQ=255)    ttGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCt                                                                     >  2:518225/1‑76 (MQ=255)    ttGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCt                                                                     <  2:334843/76‑1 (MQ=255)     tGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTa                                                                    >  2:1305023/1‑76 (MQ=255)     tGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTa                                                                    <  1:336403/76‑1 (MQ=255)      gAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTaa                                                                   <  1:1235324/76‑1 (MQ=255)        aaCTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAAc                                                                 <  2:1794256/76‑1 (MQ=255)         aCTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACg                                                                >  1:1367032/1‑76 (MQ=255)           tttCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGta                                                              <  1:1927718/76‑1 (MQ=255)           tttCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGta                                                              <  1:486215/76‑1 (MQ=255)            ttCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGtat                                                             >  2:1934821/1‑76 (MQ=255)              ctcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATaa                                                           <  1:1901375/76‑1 (MQ=255)               tcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAg                                                          <  1:1278304/76‑1 (MQ=255)               tcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAg                                                          <  1:517966/76‑1 (MQ=255)               tcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAg                                                          >  2:1369528/1‑76 (MQ=255)               tcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAg                                                          <  1:1615049/76‑1 (MQ=255)                cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGc                                                         >  2:1781246/1‑76 (MQ=255)                cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGc                                                         <  2:672709/76‑1 (MQ=255)                cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGc                                                         <  2:351505/76‑1 (MQ=255)                cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGc                                                         <  2:1925760/76‑1 (MQ=255)                    ttCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTAt                                                     >  1:1150950/1‑76 (MQ=255)                      cAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGt                                                   <  1:551248/76‑1 (MQ=255)                       aaCATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTa                                                  >  1:1483880/1‑76 (MQ=255)                         cATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAga                                                <  2:1707773/76‑2 (MQ=255)                         cATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGt                                                >  1:1905176/1‑76 (MQ=255)                         cATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGt                                                <  2:307813/76‑1 (MQ=255)                          aTGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTg                                               <  1:1426493/76‑1 (MQ=255)                          aTGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTg                                               >  1:691567/1‑76 (MQ=255)                           tGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGa                                              <  2:1593166/76‑1 (MQ=255)                             cAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAgg                                            <  1:1861973/76‑1 (MQ=255)                              aGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGt                                           >  1:668294/1‑76 (MQ=255)                               ggCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTa                                          >  1:1918225/1‑76 (MQ=255)                               ggCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTa                                          >  1:1245043/1‑76 (MQ=255)                                gCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTaa                                         <  1:453784/76‑1 (MQ=255)                                gCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTaa                                         >  1:1361286/1‑76 (MQ=255)                                 ntCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAAt                                        >  1:787494/2‑76 (MQ=255)                                 cTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAAt                                        <  1:987681/76‑1 (MQ=255)                                 cTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAAt                                        >  1:1916065/1‑76 (MQ=255)                                 cTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAAt                                        >  1:1119766/1‑76 (MQ=255)                                  tCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATc                                       >  2:1691932/1‑76 (MQ=255)                                    gACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAg                                     >  2:924190/1‑76 (MQ=255)                                    gACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAg                                     <  1:588331/76‑1 (MQ=255)                                      cATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGt                                   >  1:1342224/1‑76 (MQ=255)                                        ttGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTa                                 <  2:596517/76‑1 (MQ=255)                                         tGGCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTAt                                <  1:1605135/76‑1 (MQ=255)                                          ggCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATg                               >  1:1105040/1‑76 (MQ=255)                                           gCAAATTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGc                              <  2:1877252/76‑1 (MQ=255)                                               aTTTTCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGAt                          <  2:228853/76‑1 (MQ=255)                                                ttttCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGAtt                         >  1:377126/1‑76 (MQ=255)                                                 tttCTGGTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTc                        >  1:155877/1‑76 (MQ=255)                                                      ggTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGtgtg                   <  1:1457112/76‑1 (MQ=255)                                                       gTTATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGc                  >  1:653431/1‑76 (MQ=255)                                                        ttATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGcc                 >  2:47459/1‑76 (MQ=255)                                                        ttATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGcc                 >  2:1680020/1‑76 (MQ=255)                                                         tATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCg                <  1:1662004/76‑1 (MQ=255)                                                         tATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCg                >  2:1988075/1‑76 (MQ=255)                                                         tATCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCg                >  2:276757/1‑76 (MQ=255)                                                           tCTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGa              >  1:729056/1‑76 (MQ=255)                                                            cTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGAc             >  2:551606/1‑76 (MQ=255)                                                            cTTCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGAc             <  1:15394/76‑1 (MQ=255)                                                             ttCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACg            <  2:558109/76‑1 (MQ=255)                                                             ttCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACg            >  2:204587/1‑76 (MQ=255)                                                             ttCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACg            >  1:727932/1‑76 (MQ=255)                                                              tCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGa           >  1:955360/1‑76 (MQ=255)                                                              tCAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGa           >  1:368131/1‑76 (MQ=255)                                                               cAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAg          <  2:710971/76‑1 (MQ=255)                                                               cAGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAg          <  2:1303481/76‑1 (MQ=255)                                                                aGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGc         <  1:139969/76‑1 (MQ=255)                                                                aGCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGc         >  1:1080663/1‑76 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt        >  1:1313752/1‑76 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt        >  1:1029662/1‑76 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt        <  2:1921295/76‑1 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt        <  2:185444/76‑1 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt        <  2:1599092/76‑1 (MQ=255)                                                                 gCTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGAATCGTGTGCCGGACGAGCt        >  1:665421/1‑76 (MQ=255)                                                                  cTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTa       <  2:502127/76‑1 (MQ=255)                                                                  cTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTa       <  1:275002/76‑1 (MQ=255)                                                                  cTATCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTa       >  2:825650/1‑76 (MQ=255)                                                                     tCTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTAccc    <  2:1022171/76‑1 (MQ=255)                                                                      cTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccg   >  1:1303618/1‑76 (MQ=255)                                                                      cTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccg   >  1:1873136/1‑76 (MQ=255)                                                                      cTGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccg   >  1:563342/1‑76 (MQ=255)                                                                       tGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgc  >  2:1663618/1‑76 (MQ=255)                                                                       tGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgc  >  1:1084069/1‑76 (MQ=255)                                                                       tGGGTGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgc  <  2:1545134/76‑1 (MQ=255)                                                                          |                                                                         TCCTTGAAACTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGC  >  REL606/4193611‑4193756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑