Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893607–3894994 3901930 6937–8324 21 [19] [0] 73 [insJ‑5]–[yieO] [insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)69 (1.130) 50/146 0.2 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system

Only 100 of 102 total aligned reads displayed.

CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCAAGC  >  REL606/3652460‑3652608
                                                                         |                                                                           
cTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTTGGGTCAGTACaaa                                                                           <  2:890668/76‑1 (MQ=14)
cTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACaaa                                                                           <  2:890653/76‑1 (MQ=31)
cTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACaaa                                                                           <  2:385196/76‑1 (MQ=31)
 tGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAAc                                                                          <  2:39362/76‑1 (MQ=32)
  gACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACt                                                                         >  2:1066137/1‑76 (MQ=34)
  gACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACt                                                                         >  1:643506/1‑76 (MQ=34)
  gACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACt                                                                         <  1:27780/76‑1 (MQ=34)
  gACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACt                                                                         <  2:1890272/76‑1 (MQ=34)
    cTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACttt                                                                       >  1:919401/1‑76 (MQ=38)
    cTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACttt                                                                       >  2:787736/1‑76 (MQ=38)
     tCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTg                                                                      <  2:294742/76‑1 (MQ=39)
     tCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTg                                                                      <  1:1743619/76‑1 (MQ=39)
      ccAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGa                                                                     >  2:1052987/1‑76 (MQ=40)
      ccAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGa                                                                     >  2:687889/1‑76 (MQ=40)
      ccAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGa                                                                     >  1:1715486/1‑76 (MQ=40)
      ccAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGa                                                                     >  1:717302/1‑76 (MQ=40)
        aaTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGAtt                                                                   >  1:1219643/1‑76 (MQ=255)
        aaTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGAtt                                                                   >  1:1946649/1‑76 (MQ=255)
        aaTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGAtt                                                                   >  1:1912924/1‑76 (MQ=255)
         aTTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGAttt                                                                  >  2:1198759/1‑76 (MQ=255)
           tGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTtat                                                                >  1:1115994/1‑76 (MQ=255)
           tGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTtat                                                                >  2:1923303/1‑76 (MQ=255)
            gAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTtata                                                               >  1:452476/1‑76 (MQ=255)
            gAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTtata                                                               >  1:628571/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  1:46717/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              <  2:504862/76‑1 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  2:11435/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  2:707969/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  2:1076023/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  1:1799132/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              >  2:617143/1‑76 (MQ=255)
             aaTATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTCTTTGGGGTCAGTACAAACTTTGATTTATAg                                                              <  2:504884/76‑1 (MQ=255)
              atatCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGt                                                             >  1:1512191/1‑76 (MQ=255)
              atatCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGt                                                             <  1:636271/76‑1 (MQ=255)
                 tCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAg                                                          <  2:1906319/76‑1 (MQ=255)
                 tCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAg                                                          <  2:1487002/76‑1 (MQ=255)
                     aaTCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTgg                                                      >  1:1987496/1‑76 (MQ=255)
                      aTCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTggg                                                     >  1:1936222/1‑76 (MQ=255)
                      aTCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTggg                                                     >  2:1148372/1‑76 (MQ=255)
                        cAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGc                                                   >  2:188524/1‑76 (MQ=255)
                        cAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGc                                                   <  1:443844/76‑1 (MQ=255)
                        cAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGc                                                   >  1:1764170/1‑76 (MQ=255)
                         aGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCt                                                  <  2:1256839/76‑1 (MQ=255)
                           aCCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGTTGGGGCttt                                                <  2:504797/76‑1 (MQ=255)
                            ccTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCtttt                                               >  1:666298/1‑76 (MQ=255)
                            ccTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCtttt                                               <  1:466328/76‑1 (MQ=255)
                            ccTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCtttt                                               <  2:1993486/76‑1 (MQ=255)
                            ccTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCtttt                                               >  1:909933/1‑76 (MQ=255)
                             cTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTtc                                              >  2:1555761/1‑76 (MQ=255)
                             cTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTtc                                              <  2:102944/76‑1 (MQ=255)
                             cTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTtc                                              >  1:1021388/1‑76 (MQ=255)
                             cTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTtc                                              <  1:862464/76‑1 (MQ=255)
                                tatGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTctct                                           <  1:912356/76‑1 (MQ=255)
                                tatGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTctct                                           <  2:260591/76‑1 (MQ=255)
                                tatGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTctct                                           <  1:476299/76‑1 (MQ=255)
                                tatGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTctct                                           >  1:1449086/1‑76 (MQ=255)
                                 atGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCtctg                                          >  2:1516724/1‑76 (MQ=255)
                                  tGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCtctgt                                         >  1:1731305/1‑76 (MQ=255)
                                    ccTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCtctgtct                                       >  2:1481430/1‑76 (MQ=255)
                                     cTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCtctgtctg                                      <  2:648424/76‑1 (MQ=255)
                                       cGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGcc                                    >  1:78002/1‑76 (MQ=255)
                                       cGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGcc                                    >  2:447700/1‑76 (MQ=255)
                                        gtgtTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCt                                   >  2:836918/1‑76 (MQ=255)
                                         tgtTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCtt                                  <  1:337873/76‑1 (MQ=255)
                                         tgtTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCtt                                  <  2:1711304/76‑1 (MQ=255)
                                          gtTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCttt                                 <  1:909703/76‑1 (MQ=255)
                                           tttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTc                                <  2:1170569/76‑1 (MQ=255)
                                           tttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTc                                <  2:308518/76‑1 (MQ=255)
                                           tttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTc                                <  1:1553878/76‑1 (MQ=255)
                                           tttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTc                                >  2:546294/1‑76 (MQ=255)
                                           tttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTc                                >  1:1308050/1‑76 (MQ=255)
                                            ttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCg                               <  1:1515987/76‑1 (MQ=255)
                                            ttAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCg                               <  1:1838447/76‑1 (MQ=255)
                                             tAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCgg                              >  2:261818/1‑76 (MQ=255)
                                                 tGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGaa                          >  1:690867/1‑76 (MQ=255)
                                                  gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAAt                         >  1:262342/1‑76 (MQ=255)
                                                  gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAAt                         >  1:620965/1‑76 (MQ=255)
                                                  gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAAt                         <  1:158880/76‑1 (MQ=255)
                                                  gTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAAt                         >  2:582608/1‑76 (MQ=255)
                                                   tCCAACTTTTTGGGGTCAGTACAAACTCTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATa                        >  1:1424671/1‑76 (MQ=255)
                                                    ccAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATAc                       <  1:1078766/76‑1 (MQ=255)
                                                    ccAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATAc                       >  1:1478573/1‑76 (MQ=255)
                                                       aCTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATATCTg                    <  2:1969285/76‑1 (MQ=255)
                                                       aCTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTg                    <  1:1820013/76‑1 (MQ=255)
                                                           tttGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGAc                >  1:566343/1‑76 (MQ=255)
                                                                  tCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGt         >  2:1630662/1‑76 (MQ=255)
                                                                  tCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGt         <  2:379443/76‑1 (MQ=255)
                                                                  tCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGt         <  2:436659/76‑1 (MQ=255)
                                                                    aGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtct       >  2:1449595/1‑76 (MQ=255)
                                                                    aGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtct       >  1:20654/1‑76 (MQ=255)
                                                                     gTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtctc      <  2:1261039/76‑1 (MQ=255)
                                                                     gTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtctc      <  2:468239/76‑1 (MQ=255)
                                                                     gTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtctc      <  1:1207145/76‑1 (MQ=255)
                                                                     gTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGtctc      <  2:473716/76‑1 (MQ=255)
                                                                      tACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCa     >  2:561165/1‑76 (MQ=255)
                                                                      tACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCa     <  1:1133720/76‑1 (MQ=255)
                                                                       aCAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCaa    >  1:1173996/1‑76 (MQ=255)
                                                                       aCAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCaa    <  2:832867/76‑1 (MQ=255)
                                                                         aaaCTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCAAGc  >  1:586912/1‑76 (MQ=255)
                                                                         aaaCTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCAAGc  >  2:1117062/1‑76 (MQ=255)
                                                                         |                                                                           
CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATACCTGAGACAAACAGTCTCAAGC  >  REL606/3652460‑3652608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.