Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,563,0850TG32.6% 72.1 / 19.5 46T193T (ACA→ACCyfgAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/26);  new base G (15/0);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 3.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTCG  >  REL606/2563012‑2563158
                                                                         |                                                                         
cATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGgtgt                                                                         <  1:23156/76‑1 (MQ=255)
cATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGgggt                                                                         >  2:1573569/1‑76 (MQ=255)
  tttGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGgtt                                                                       >  2:428253/1‑76 (MQ=255)
  tttGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGttt                                                                       >  1:1399651/1‑76 (MQ=255)
   ttGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTg                                                                      >  2:1318821/1‑76 (MQ=255)
    tGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGGTTgg                                                                     >  2:1790380/1‑75 (MQ=255)
       cTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCgtg                                                                  <  2:793213/76‑1 (MQ=255)
       cTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGGTTGCggg                                                                  >  2:57920/1‑74 (MQ=255)
         gCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGGTTGGGTgtt                                                                >  2:712416/1‑76 (MQ=255)
              ggCGAACCAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTgttggttg                                                           <  2:1562553/76‑1 (MQ=255)
               gCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGc                                                          <  1:123812/76‑1 (MQ=255)
                   aacaacCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGgta                                                      >  2:1374635/1‑76 (MQ=255)
                     caacCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGGTTGCGGGGTGGTTGCGggagg                                                    >  2:1579520/1‑75 (MQ=255)
                     aaacCGCATTCTGTTGCGGATCAACAGGTGGTGCTGGAGAAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGgtagt                                                    <  2:420802/75‑1 (MQ=255)
                       acCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTAt                                                  <  1:265198/76‑1 (MQ=255)
                           cATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCcac                                              <  2:1224899/76‑1 (MQ=255)
                            aTTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGGTTGCGTGTTGGTTGCGGTAGTATCcaca                                             >  1:1518342/1‑76 (MQ=255)
                            aTTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGGTTGCGTGTTGGGTGCGGGAGTATCcacc                                             >  1:221773/1‑75 (MQ=255)
                            aTTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGTAGTATCcaca                                             >  1:1532816/1‑76 (MQ=255)
                            aTTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGGTGCGGTAGTATCcacc                                             >  1:1085108/1‑75 (MQ=255)
                               cTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGAc                                          <  2:1522094/76‑1 (MQ=255)
                               cTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGGGTTGGTTGCGGGAGTATCCACAGAc                                          >  1:1978679/1‑76 (MQ=255)
                                tGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGGTTGCGGGTTGGGTGCGGGAGTATCCACAGACg                                         >  2:1002950/1‑76 (MQ=255)
                                  ttGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGGTGCGGGAGTATCCACCAACGcc                                       >  2:1225576/1‑75 (MQ=255)
                                  ttGCGGATCAACAGCTGGTGCTGGAGCAGTTACGGCAGGTGTTTTCGTGTTGGTTGCGGTAGTATCCACAGACGcc                                       >  2:1937211/1‑75 (MQ=255)
                                   tGCGGATCAACAGCTGGTGCTGGCGTAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGTATCCACAGACGCTg                                      >  2:1805208/1‑76 (MQ=255)
                                   tGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGGGGGAGTATCCACAGACGCTg                                      >  1:680014/1‑76 (MQ=255)
                                      ggATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTggcg                                   <  1:914384/76‑1 (MQ=255)
                                       gATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGTATCCACAGACGCTggcgg                                  >  1:1769397/1‑76 (MQ=255)
                                        aTCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGGGGTAGGATCCACAGACGCcggcggg                                 >  2:332906/1‑75 (MQ=255)
                                        aTCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGTATCCACAGACGCTgggggg                                 >  1:286079/1‑75 (MQ=255)
                                        aTCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGGTGCGGGAGGATCCACAGACGCTgggggg                                 >  1:1252801/1‑75 (MQ=255)
                                         tCAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGGATCCACAGACGCTGgcggcg                                >  1:465133/1‑76 (MQ=255)
                                          cAACAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGTATCCACAGACGCTGgcggcgg                               >  2:282635/1‑75 (MQ=255)
                                            ccggCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTgg                             <  2:1318529/73‑1 (MQ=255)
                                            aCAGCTGGTGCTGGCGCAGTTACGGCGGGTGTTTGCGTGTTGGGTGCGGTAGTATCCACAGACGCTGGCGGCGTgg                             <  2:660819/76‑1 (MQ=255)
                                            aCAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTgg                             <  1:799592/76‑1 (MQ=255)
                                             cAGCTGGTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCggggg                            >  1:923909/1‑75 (MQ=255)
                                              aGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGAc                           <  2:1501122/76‑1 (MQ=255)
                                               gcgggTGCTGGCGAAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTAGCGGCGTGGACg                          <  2:1305919/73‑1 (MQ=255)
                                               gCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACg                          <  2:1051863/76‑1 (MQ=255)
                                                  ggTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTg                       <  2:1529309/76‑1 (MQ=255)
                                                  ggTGCTGGCGCAGTTACGGCAGGGGTTTGCGGGTTGGTTGCGGTAGTATCCACAGACGCTGGGGGCGTGGGCGGTg                       >  2:339743/1‑76 (MQ=255)
                                                   gTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGt                      <  2:985032/76‑1 (MQ=255)
                                                   gTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGt                      <  1:201274/76‑1 (MQ=255)
                                                   gTGCTGGCGCAGTTACGGCAGGGGTTTGCGTGGTGGTTGCGGTAGTATTCCCAGGAGCTTGCGGCGCGGTCGgtgt                      >  2:1419071/1‑76 (MQ=255)
                                                    tGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTa                     <  2:954888/76‑1 (MQ=255)
                                                      cTGGCGGAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCGGGCGGCGTGGACGTTGTAGc                   <  1:410643/76‑1 (MQ=255)
                                                       tGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGcc                  <  1:1972680/76‑1 (MQ=255)
                                                         gcgcAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCgg                <  2:200682/76‑1 (MQ=255)
                                                             aGTTACGGCTGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCt            <  1:1440016/76‑1 (MQ=255)
                                                              gTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTg           <  1:487486/76‑1 (MQ=255)
                                                                tACGGCAGGGGTTTGCGTGTTGGTTGCGGGAGTATCCACAGACGCTGGCGGGGGGGACGGTGTGGCCGGGGCTGTa         >  2:88390/1‑76 (MQ=255)
                                                                  cGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGt       <  1:1823416/76‑1 (MQ=255)
                                                                  cGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGt       >  2:66438/1‑76 (MQ=255)
                                                                  cGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGt       <  1:1859377/76‑1 (MQ=255)
                                                                   ggCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGtt      <  1:8835/76‑1 (MQ=255)
                                                                    ggaGGTTTTTGCGTGTGGGTTGCGGTAGTGTCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTg     <  2:394914/74‑1 (MQ=255)
                                                                    gCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTg     <  2:613512/76‑1 (MQ=255)
                                                                     cAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGt    >  2:465459/1‑76 (MQ=255)
                                                                      gggTGTTTGCGTGTTGTTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTc   <  2:1196381/75‑1 (MQ=255)
                                                                      aGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTc   <  2:372867/76‑1 (MQ=255)
                                                                      aGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTc   >  2:1865435/1‑76 (MQ=255)
                                                                       ggTGTTTGCGTGTTGGTGGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTCg  <  2:1427668/76‑1 (MQ=255)
                                                                         |                                                                         
CATTTGCCTGCGAAGGCGAAACAACCGCATTCTGTTGCGGATCAACAGCTGGTGCTGGCGCAGTTACGGCAGGTGTTTGCGTGTTGGTTGCGGTAGTATCCACAGACGCTGGCGGCGTGGACGTTGTAGCCGGGTCTGTAGTTGTCG  >  REL606/2563012‑2563158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: