Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,872,885	G→T	R384S (CGC→AGC)	glmS ←	D‑fructose‑6‑phosphate amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,872,885	0	G	T	100.0%	322.9 / NA	82	R384S (CGC→AGC)	glmS	D‑fructose‑6‑phosphate amidotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/34);  total (48/34)

GGTGAATGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCGCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGACGCAG  >  REL606/3872814‑3872957                                                                        |                                                                         ggtgAATGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCAcc                                                                      <  2:751658/76‑1 (MQ=255)   tgAATGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCag                                                                    >  2:898929/1‑76 (MQ=255)      aTGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAgaga                                                                 <  2:888969/76‑1 (MQ=255)       tGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGagaa                                                                >  1:731816/1‑76 (MQ=255)       tGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGagaa                                                                <  1:687343/76‑1 (MQ=255)       tGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGagaa                                                                >  1:1718961/1‑76 (MQ=255)       tGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGagaa                                                                <  1:1636029/76‑1 (MQ=255)        gCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGagaag                                                               >  2:955916/1‑76 (MQ=255)          tttAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAgaagaa                                                             <  2:450676/76‑1 (MQ=255)          tttAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAgaagaa                                                             <  1:908758/76‑1 (MQ=255)          tttAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGGCATTAGCGCCAGATCGGATTCGCTCACCAGCgaagaa                                                             >  2:1177105/1‑76 (MQ=255)               tGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCgg                                                        >  1:1809480/1‑76 (MQ=255)                ggATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGa                                                       >  1:1458094/1‑76 (MQ=255)                ggATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGa                                                       >  2:1793846/1‑76 (MQ=255)                  aTGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAAc                                                     >  1:837273/1‑76 (MQ=255)                  aTGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAAc                                                     <  2:1096047/76‑1 (MQ=255)                     ccACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGtt                                                  >  2:450194/1‑76 (MQ=255)                     ccACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGtt                                                  >  2:1089251/1‑76 (MQ=255)                     ccACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGtt                                                  >  2:497323/1‑76 (MQ=255)                     ccACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGtt                                                  >  2:1867511/1‑76 (MQ=255)                      cACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTa                                                 >  1:1152923/1‑76 (MQ=255)                        cGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACa                                               >  2:614054/1‑76 (MQ=255)                         gCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAg                                              <  1:770710/76‑1 (MQ=255)                          ccGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGa                                             <  1:887763/76‑1 (MQ=255)                          ccGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGa                                             >  1:1617583/1‑76 (MQ=255)                          ccGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGa                                             >  1:685047/1‑76 (MQ=255)                             aTTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTg                                          >  2:252480/1‑76 (MQ=255)                              tttCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGc                                         >  2:1974994/1‑76 (MQ=255)                               ttCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGcc                                        >  1:1563969/1‑76 (MQ=255)                               ttCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGcc                                        <  1:1233387/76‑1 (MQ=255)                                tCTGTCCCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCa                                       <  1:795337/76‑1 (MQ=255)                                tCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCa                                       <  1:61990/76‑1 (MQ=255)                                tCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCa                                       <  2:1668624/76‑1 (MQ=255)                                tCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCa                                       >  1:1709355/1‑76 (MQ=255)                                tCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCa                                       <  2:104658/76‑1 (MQ=255)                                  tGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGt                                     >  2:1141622/1‑76 (MQ=255)                                    tccccGCTTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGa                                   <  2:491351/74‑1 (MQ=255)                                    tACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGa                                   >  1:730274/1‑76 (MQ=255)                                     aCCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGaa                                  >  2:1306448/1‑76 (MQ=255)                                     aCCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGaa                                  >  1:1912778/1‑76 (MQ=255)                                      cccGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAAc                                 <  2:621574/76‑1 (MQ=255)                                      cccGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAAc                                 <  2:1120335/76‑1 (MQ=255)                                       ccGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAAcc                                >  2:1462923/1‑76 (MQ=255)                                       ccGCGTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAAcc                                <  2:1782124/76‑1 (MQ=255)                                          cgTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAg                             <  2:649516/76‑1 (MQ=255)                                          cgTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAg                             >  1:1107480/1‑76 (MQ=255)                                           gTTGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAgg                            >  2:1564496/1‑76 (MQ=255)                                            ttGGTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGt                           >  1:551913/1‑76 (MQ=255)                                              ggTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTaa                         >  2:1055287/1‑76 (MQ=255)                                              ggTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTaa                         <  1:1012840/76‑1 (MQ=255)                                              ggTCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTaa                         <  1:1516099/76‑1 (MQ=255)                                                tCATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACAAGGGAAAcc                       >  2:374813/1‑76 (MQ=255)                                                 cATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAAccc                      <  2:1250172/76‑1 (MQ=255)                                                 cATTAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAAccc                      <  1:1966265/76‑1 (MQ=255)                                                   ttAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAg                    <  2:1103580/76‑1 (MQ=255)                                                   ttAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAg                    >  2:114899/1‑76 (MQ=255)                                                   ttAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAg                    <  2:1780746/76‑1 (MQ=255)                                                   ttAGCGCCAGATCGGATTCGCTCACAAG‑GAAGAACCCGGGACGGTACAGATTGCCAGGGGACCAAAGGAACCCaag                   >  2:293548/1‑74 (MQ=255)                                                    tAGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGc                   >  2:1741104/1‑76 (MQ=255)                                                     aGCGCCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGCAACCCAGct                  >  1:871559/1‑76 (MQ=255)                                                        gcCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCtt               <  2:1900245/76‑1 (MQ=255)                                                        gcCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCtt               >  1:1401771/1‑76 (MQ=255)                                                        gcCAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCtt               >  2:683225/1‑76 (MQ=255)                                                         ccAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCttt              >  1:1459767/1‑76 (MQ=255)                                                         ccAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCttt              >  2:199040/1‑76 (MQ=255)                                                         ccAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCttt              >  2:357867/1‑76 (MQ=255)                                                          cAGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTc             >  1:952173/1‑76 (MQ=255)                                                           aGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCg            >  1:176466/1‑76 (MQ=255)                                                           aGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCg            >  2:382696/1‑76 (MQ=255)                                                           aGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCg            >  1:1614531/1‑76 (MQ=255)                                                           aGATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCg            <  1:1974995/76‑1 (MQ=255)                                                            gATCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGa           <  2:233458/76‑1 (MQ=255)                                                              tCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACa         <  2:27805/76‑1 (MQ=255)                                                              tCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACa         >  2:369832/1‑76 (MQ=255)                                                              tCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACa         <  1:44564/76‑1 (MQ=255)                                                              tCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACa         <  2:1567975/76‑1 (MQ=255)                                                              tCGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACa         >  2:1061166/1‑76 (MQ=255)                                                               cGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAg        >  2:960031/1‑76 (MQ=255)                                                               cGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAg        <  2:344959/76‑1 (MQ=255)                                                               cGGATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAg        <  2:1316896/76‑1 (MQ=255)                                                                 gATTCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGAc      <  1:202704/76‑1 (MQ=255)                                                                   ttCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGACGc    >  1:1931627/1‑76 (MQ=255)                                                                    tCGCTCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGACGCa   >  1:1364782/1‑76 (MQ=255)                                                                     cgctCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGACGCAg  <  2:1107479/76‑1 (MQ=255)                                                                        |                                                                         GGTGAATGCTTTAGTGGATGCCACGCCGATTTCTGTACCCGCGTTGGTCATTAGCGCCAGATCGGATTCGCGCACCAGAGAAGAACCCGGAACGTTACAGATTGCCAGTGAACCAAGGTAACCCAGCTCTTTCGACAGACGCAG  >  REL606/3872814‑3872957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑