| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,569 | A→T | I572F (ATC→TTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,569 | 0 | A | T | 98.7% | 294.3 / ‑3.7 | 74 | I572F (ATC→TTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (35/38); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 | cGAGACGTACACCCGACTCACTATGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:688751/1‑76 (MQ=255)cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 1:682146/1‑76 (MQ=255)cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:225638/1‑76 (MQ=255)cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCa > 2:1161305/1‑76 (MQ=255) agaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAAc < 2:778928/76‑1 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAActctct < 2:349393/76‑1 (MQ=255) acccccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTg < 2:1632125/73‑1 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTg < 2:1690448/76‑1 (MQ=255) cccccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt < 2:657926/74‑1 (MQ=255) cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGCACATCGGGCTGTTCAACTCTCTGt > 1:968339/1‑76 (MQ=255) cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGt < 1:173247/76‑1 (MQ=255) ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCg < 2:1918022/76‑1 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCgtgt < 2:778927/76‑1 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCgtgt < 2:876531/76‑1 (MQ=255) cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTa < 2:842658/76‑1 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACg > 2:1204204/1‑76 (MQ=255) cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACg < 1:542811/76‑1 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGcaca > 2:469205/1‑76 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGcaca < 1:1181281/76‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAg < 1:260619/76‑1 (MQ=255) cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAg < 1:69483/76‑1 (MQ=255) ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGa > 2:962088/1‑76 (MQ=255) gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGAc < 2:696018/76‑1 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACt > 2:247176/1‑76 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGCCTa < 2:1121226/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTa > 1:323404/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 2:1875481/76‑1 (MQ=255) gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTaa < 1:74970/76‑1 (MQ=255) gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACg > 1:1054739/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1338906/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 2:1519865/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa < 1:1526975/76‑1 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:1012789/1‑76 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 2:997391/1‑76 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa > 2:178747/1‑76 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:513744/76‑1 (MQ=255) tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc > 2:34406/1‑76 (MQ=255) ctaaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:494841/3‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:1196839/76‑1 (MQ=255) aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc < 2:294615/76‑1 (MQ=255) aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 2:1604464/76‑1 (MQ=255) gAAACCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc > 2:1097730/1‑76 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 1:1310502/1‑76 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:876062/76‑1 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 2:1062955/76‑1 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt < 1:340779/76‑1 (MQ=255) aCCCCTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 2:450911/76‑1 (MQ=255) cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:1451833/76‑1 (MQ=255) cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 2:624781/76‑1 (MQ=255) cccTGAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag < 1:214618/76‑1 (MQ=255) gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 2:866837/1‑76 (MQ=255) gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc < 2:1851100/76‑1 (MQ=255) gAAGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc < 1:676054/76‑1 (MQ=255) naGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc > 1:468519/2‑76 (MQ=255) aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg > 2:1082964/1‑76 (MQ=255) aGGTCCGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg > 2:1906926/1‑76 (MQ=255) cGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg > 1:387903/1‑76 (MQ=255) cGAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg < 2:1449581/76‑1 (MQ=255) gAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt > 1:1711300/1‑76 (MQ=255) gAACATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt > 1:489360/1‑76 (MQ=255) aaCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa > 1:1738463/1‑76 (MQ=255) aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa > 1:1200629/1‑76 (MQ=255) aCATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa < 2:1568698/76‑1 (MQ=255) cATCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa > 2:551427/1‑76 (MQ=255) aTCGGTCTGTTCAACTCTCTGTCCGTGTACGGACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:1131874/76‑1 (MQ=255) aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 2:450360/76‑1 (MQ=255) aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:1338507/1‑76 (MQ=255) aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:723670/1‑76 (MQ=255) aTCGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg < 1:361532/76‑1 (MQ=255) cGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:106608/1‑76 (MQ=255) cGGTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:1723056/76‑1 (MQ=255) gTCTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCTTAAAGTGAc > 1:563179/1‑76 (MQ=255) cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:1277145/76‑1 (MQ=255) cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg > 1:961671/1‑76 (MQ=255) cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 1:684812/76‑1 (MQ=255) cTGTTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCCTGAGACTCCGTATCGTAAAGTGACCg > 1:1255337/1‑76 (MQ=255) | CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |