Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,479	A→C	V254G (GTA→GGA)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,479	0	A	C	100.0%	174.2 / NA	42	V254G (GTA→GGA)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/20);  total (22/20)

CAGGAATAAATAATCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATC  >  REL606/1305407‑1305549                                                                          |                                                                       cAGGAATAAATAATCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGc                                                                     >  2:66719/1‑76 (MQ=255)  aGGAATAAATAATCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGcc                                                                    >  1:949728/1‑76 (MQ=255)     aataaataATCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTccgcccgc                                                                 >  2:1276739/1‑76 (MQ=255)        aaataaTCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATc                                                              >  2:1636999/1‑76 (MQ=255)        aaataaTCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATc                                                              >  2:1766605/1‑76 (MQ=255)          ataatCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCtt                                                            >  2:1138967/1‑76 (MQ=255)             atCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGtt                                                         >  2:1777837/1‑76 (MQ=255)             atCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGtt                                                         >  2:1832680/1‑76 (MQ=255)               ccccATCGCCGGGGCCGTGGGGCCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGttt                                                        <  2:496204/76‑1 (MQ=255)                  cATCGGGGGGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGaan                                                    <  1:103772/76‑2 (MQ=255)                   aTCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCCCCTTGTTTGAAGt                                                   >  1:1481234/1‑76 (MQ=255)                     cggggTGGCGTGGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAg                                                 <  1:1883531/73‑1 (MQ=255)                     cgcgGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAg                                                 >  2:1488631/1‑76 (MQ=255)                          tGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAgg                                            >  1:390102/1‑76 (MQ=255)                          tGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAgg                                            >  2:417597/1‑76 (MQ=255)                              ggggggc‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCg                                        <  2:1802537/73‑1 (MQ=255)                              gATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTATCGGGGGtgg                                        >  1:623880/1‑74 (MQ=255)                                 gggc‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGAcc                                     <  1:977384/76‑1 (MQ=255)                                      ccTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCatat                                 >  2:1872131/1‑76 (MQ=255)                                       cTTCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATAtt                                <  1:939389/76‑1 (MQ=255)                                        ttCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTc                               <  1:1102079/76‑1 (MQ=255)                                         tCCATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCa                              <  2:657876/76‑1 (MQ=255)                                          ccATACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAt                             >  1:1268361/1‑76 (MQ=255)                                            aTACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgc                           >  1:478043/1‑76 (MQ=255)                                             tACGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgct                          >  1:1055452/1‑76 (MQ=255)                                              aCGCGCCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCAtgctg                         >  2:1996679/1‑76 (MQ=255)                                                 cgcCATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCg                      <  2:1938484/76‑1 (MQ=255)                                                    cATCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGgtg                   >  1:862743/1‑76 (MQ=255)                                                      tCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTa                 <  2:89056/76‑1 (MQ=255)                                                      tCAGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTa                 <  2:1152648/76‑1 (MQ=255)                                                        aGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTAcg               <  1:635371/76‑1 (MQ=255)                                                        aGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTAcg               <  1:695091/76‑1 (MQ=255)                                                        aGATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCAACCATATTCATGCTGCCGGTGTAcg               <  1:29279/76‑1 (MQ=255)                                                         gATCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTAcgc              <  2:1334558/76‑1 (MQ=255)                                                           tCAATCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGa            >  2:1290037/1‑76 (MQ=255)                                                               tCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTa        <  2:1794648/76‑1 (MQ=255)                                                               tCCATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTa        <  2:1350187/76‑1 (MQ=255)                                                                ccATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTaa       <  1:1637386/76‑1 (MQ=255)                                                                 cATTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAAt      >  1:821571/1‑76 (MQ=255)                                                                  aTTGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAAtt     <  1:693137/76‑1 (MQ=255)                                                                   ttGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTa    <  2:300325/76‑1 (MQ=255)                                                                    tGCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTAt   >  1:1614894/1‑76 (MQ=255)                                                                     gCCCTCCGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATc  >  1:1893478/1‑76 (MQ=255)                                                                          |                                                                       CAGGAATAAATAATCCCCATCGCGGTGGCGATGGGG‑CCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATC  >  REL606/1305407‑1305549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑