| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,570,953 | G→T | D52Y (GAT→TAT) | uspA → | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,570,953 | 0 | G | T | 97.5% | 299.0 / 1.5 | 79 | D52Y (GAT→TAT) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (38/39); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCACTA > REL606/3570880‑3571025 | tCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCttatttat > 2:1252751/1‑76 (MQ=255) cGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTAtg < 1:639638/76‑1 (MQ=255) cGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTAtg > 2:722091/1‑76 (MQ=255) cccTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGa > 2:1001666/1‑76 (MQ=255) cTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAAt > 2:1576322/1‑76 (MQ=255) tACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATc < 1:433434/76‑1 (MQ=255) aCAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCt < 1:1710983/76‑1 (MQ=255) cAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTg < 2:1856629/76‑1 (MQ=255) cAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTg > 2:1018159/1‑76 (MQ=255) aaTGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTgg > 1:257262/1‑76 (MQ=255) tGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGt < 2:1159226/76‑1 (MQ=255) aaaGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGata < 1:1924353/76‑1 (MQ=255) aaGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCATATTTATGTGAATCTGGGTGatat > 1:1873918/1‑76 (MQ=255) tgttCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGc < 1:1773648/74‑1 (MQ=255) tttCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCa < 2:1296166/76‑1 (MQ=255) tttCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATATGAATCTGGGTGATATGCa < 2:1120205/76‑1 (MQ=255) ttCTCTTATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAg < 1:1245280/76‑1 (MQ=255) ttCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAg < 1:1833748/76‑1 (MQ=255) tctGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGaaa < 1:74920/76‑1 (MQ=255) ctGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAAc > 1:1126797/1‑76 (MQ=255) gATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGc > 1:826168/1‑76 (MQ=255) aTCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCa > 1:36507/1‑76 (MQ=255) aTCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCa > 1:1352147/1‑76 (MQ=255) aTCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCa > 1:1409899/1‑76 (MQ=255) tCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCAt > 1:1084180/1‑76 (MQ=255) ccACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCAtc < 2:475211/76‑1 (MQ=255) cACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCAtct > 2:1807198/1‑76 (MQ=255) cACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCAtct > 2:435257/1‑76 (MQ=255) cGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCa < 1:1812654/76‑1 (MQ=255) gTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCag < 2:1924019/76‑1 (MQ=255) gTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCag > 1:675205/1‑76 (MQ=255) tAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCaga < 1:259759/76‑1 (MQ=255) tAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCaga > 1:113593/1‑76 (MQ=255) tAGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCaga > 1:1575862/1‑76 (MQ=255) aGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCagaa > 1:678280/1‑76 (MQ=255) aGATGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCagaa > 1:88754/1‑76 (MQ=255) tGTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAagag < 1:123014/76‑1 (MQ=255) gTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAgaga < 1:1001667/76‑1 (MQ=255) gTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAgaga > 1:1123529/1‑76 (MQ=255) gTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAgaga < 1:544953/76‑1 (MQ=255) gTAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAgaga > 1:657082/1‑76 (MQ=255) tAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGac > 1:1226225/1‑76 (MQ=255) tAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGac > 1:513408/1‑76 (MQ=255) tAAACTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGac > 2:1730906/1‑76 (MQ=255) aaaCTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGaca > 2:234239/1‑76 (MQ=255) aaaCTACTCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGaca > 1:1526836/1‑76 (MQ=255) aaCTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGacac < 2:840183/76‑1 (MQ=255) actCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACAcatca < 1:1121960/76‑1 (MQ=255) actCTGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACAcatca > 1:1492101/1‑76 (MQ=255) ctctGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACAcatcat > 2:568321/1‑76 (MQ=255) ctctGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACAcatcat < 2:973215/76‑1 (MQ=255) ctctGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACAcatcat < 1:143362/76‑1 (MQ=255) ctGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATgc < 2:1315592/76‑1 (MQ=255) tGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATgcg < 1:795128/76‑1 (MQ=255) tGACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATgcg < 2:1462303/76‑1 (MQ=255) gACCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATgcgc > 1:1753194/1‑76 (MQ=255) aCCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCt > 1:18148/1‑76 (MQ=255) aCCTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCt < 2:1443509/76‑1 (MQ=255) aCCTATACACCGGGCTTATTTATGTGAATCTGAGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCt < 2:1443515/76‑1 (MQ=255) aCCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCt > 2:551490/1‑76 (MQ=255) cTATACACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGa < 1:1883399/76‑1 (MQ=255) ataCACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGAcc < 1:1618922/76‑1 (MQ=255) taCACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCg > 1:34559/1‑76 (MQ=255) taCACCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCg < 1:188333/76‑1 (MQ=255) acacCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGa < 2:1918694/76‑1 (MQ=255) acacCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGa > 2:1821468/1‑76 (MQ=255) acacCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGa > 2:1319480/1‑76 (MQ=255) cacCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAg < 1:1573549/76‑1 (MQ=255) acCGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGc > 1:176004/1‑76 (MQ=255) ccGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCt > 1:1325143/1‑76 (MQ=255) ccGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCt < 1:104582/76‑1 (MQ=255) cGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCtt > 1:600095/1‑76 (MQ=255) cGGGCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCtt < 1:907514/76‑1 (MQ=255) gggCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCttt < 1:1756109/76‑1 (MQ=255) ggCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTc > 1:1555611/1‑76 (MQ=255) gCTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTcc < 1:372900/76‑1 (MQ=255) cTTATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCa > 1:1862122/1‑76 (MQ=255) ttatttatGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCAc > 1:1268856/1‑76 (MQ=255) ttatttatGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCAc > 2:957226/1‑76 (MQ=255) tATTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCACt < 2:1422653/76‑1 (MQ=255) aTTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCACTa < 1:1950107/76‑1 (MQ=255) aTTTATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACAGCATGCGCTGACCGAGCTTTCCACtg < 1:547747/76‑2 (MQ=255) | TCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGAATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCACTA > REL606/3570880‑3571025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |