| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,201,945 | Δ1 bp | coding (615/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,945 | 0 | G | . | 96.4% | 258.9 / 4.8 | 55 | coding (615/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (21/32); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 | |||||||||||
AAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTT > REL606/4201877‑4202019 | aaaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGACTAACCCCGTTT‑CGCGTTTg > 2:772802/1‑76 (MQ=255)aaaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTg > 2:1806062/1‑76 (MQ=255) aaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTgg > 2:1820924/1‑76 (MQ=255) aaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTggg < 2:1274565/76‑1 (MQ=255) aTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGc < 2:620398/76‑1 (MQ=255) acacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCga < 1:628559/76‑1 (MQ=255) acGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCgaga > 2:1663850/1‑76 (MQ=255) cGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGGTTGGGCGAGAt > 1:874355/1‑76 (MQ=255) gCTGCAAGGCAACGTAGCCCCAGCGCATGTNCCTCATCGTCAAATGAATNACNCCGTTT‑CGCGTTTGNGCGAGAtc > 1:1911718/1‑76 (MQ=255) tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGAtctt < 2:291528/76‑1 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGAtcttc > 2:603842/1‑76 (MQ=255) cAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGAtcttct < 1:651124/76‑1 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATcttctt < 1:2012583/76‑1 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCttt > 2:1952748/1‑76 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCttt < 2:551027/76‑1 (MQ=255) ggCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCtttt < 2:755249/76‑1 (MQ=255) gCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTa < 1:1916432/76‑1 (MQ=255) gCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTa < 1:742411/76‑1 (MQ=255) cGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAAt < 2:1199419/76‑1 (MQ=255) cGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATg < 1:278121/76‑1 (MQ=255) tAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGca < 2:930548/76‑1 (MQ=255) tAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGca > 1:95570/1‑76 (MQ=255) gCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGcaca < 2:270643/76‑1 (MQ=255) ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGcacan < 1:216891/76‑2 (MQ=255) ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAg > 2:1652053/1‑76 (MQ=255) cccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:402059/76‑1 (MQ=255) cccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:1385163/76‑1 (MQ=255) cAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAggcg > 1:900683/1‑74 (MQ=255) cAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag < 1:978456/76‑1 (MQ=255) cgcATGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGaca < 2:1397487/76‑1 (MQ=255) aTGTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCa < 1:1370097/76‑1 (MQ=255) gTTCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGc < 1:1730890/76‑1 (MQ=255) ttCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCg < 2:1106039/76‑1 (MQ=255) ttCCTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCg > 2:1737184/1‑76 (MQ=255) ccTCATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGtt < 1:234599/76‑1 (MQ=255) cATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCg < 2:246452/76‑1 (MQ=255) cATCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCg > 2:703231/1‑76 (MQ=255) aTCGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGt > 2:120182/1‑76 (MQ=255) cGTCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 1:2021413/1‑76 (MQ=255) tCAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 2:865460/76‑1 (MQ=255) cAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgggca > 2:648531/1‑74 (MQ=255) cAAATGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGta < 2:52485/76‑1 (MQ=255) aaaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtat < 2:1795650/76‑1 (MQ=255) aaaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtat < 2:1600932/76‑1 (MQ=255) aaaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtat < 2:1696265/76‑1 (MQ=255) aaaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGgat > 1:1025908/1‑76 (MQ=255) aaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtata < 2:1902794/76‑1 (MQ=255) aaTGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtata > 2:508425/1‑76 (MQ=255) tGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAgg > 1:287492/1‑76 (MQ=255) tGAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAgg < 1:1592159/76‑1 (MQ=255) gAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTTAATGCACAGGAGACACCAGCGTTGCGTGGGGATAGGc > 1:995369/1‑76 (MQ=255) gAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc > 1:1908088/1‑76 (MQ=255) gAATAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc > 1:161596/1‑76 (MQ=255) aaaTAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc < 1:867558/75‑1 (MQ=255) tAACCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATg < 1:1637951/76‑1 (MQ=255) aCCCCGTTT‑CGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTa < 2:968249/76‑1 (MQ=255) tGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt > 1:1658305/1‑76 (MQ=255) | AAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTT > REL606/4201877‑4202019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |