| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,486 | T→C | T274A (ACG→GCG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,486 | 0 | T | C | 97.6% | 159.9 / ‑3.9 | 42 | T274A (ACG→GCG) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (19/22); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
AGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAA > REL606/474413‑474558 | aGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGCCAGCGCAt > 2:2020481/1‑76 (MQ=255) aTTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGt < 2:224035/76‑1 (MQ=255) aTTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGt > 2:1483810/1‑76 (MQ=255) ttAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTg > 2:1220459/1‑76 (MQ=255) tAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTgg < 1:67823/76‑1 (MQ=255) aaCGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTggg > 2:284156/1‑76 (MQ=255) aaCGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTggg > 2:1582138/1‑76 (MQ=255) aaCGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTggg < 1:272164/76‑1 (MQ=255) ctgATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGggcg > 1:710861/3‑76 (MQ=255) gATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGggcggc > 1:9547/1‑76 (MQ=255) tCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCAc < 2:1235810/76‑1 (MQ=255) caacaaCCCGACGGAGACAAAAAACAGCACCGCAAACGCGGCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGa < 1:971761/76‑1 (MQ=255) caTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGCGGGCGGCGCGGCGACTc > 2:141437/1‑76 (MQ=255) aTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGCCAGCGCACCGCGGGCGCCACGGTGACTcc > 1:666741/1‑75 (MQ=255) tCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTccg > 2:1651216/1‑74 (MQ=255) gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCa > 2:971260/1‑76 (MQ=255) gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCa > 1:1530157/1‑76 (MQ=255) gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCa > 1:449777/1‑76 (MQ=255) aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGTGCGGCACGGTGACTCAGTTCAg < 2:294421/76‑1 (MQ=255) gagaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACt < 2:2064623/76‑1 (MQ=255) aaaaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTc < 1:1331030/76‑1 (MQ=255) aaaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa < 2:1835173/76‑1 (MQ=255) aaaaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa < 1:251325/76‑1 (MQ=255) cACCGCTAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATc < 1:821755/76‑1 (MQ=255) cGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCg < 1:1325569/76‑1 (MQ=255) cGCAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCg > 1:1622167/1‑76 (MQ=255) cAAACGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGc < 2:2053462/76‑1 (MQ=255) aaaCGCGTCGCGCAATGGCAGCGCATCGTGGGCTGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCa < 1:197195/76‑1 (MQ=255) aaaCGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCa < 2:76111/76‑1 (MQ=255) aaaCGCGTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCa > 1:158702/1‑76 (MQ=255) cgcgTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg < 1:1829776/76‑1 (MQ=255) cgcgTCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg < 2:441744/76‑1 (MQ=255) tCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg < 1:1407402/76‑1 (MQ=255) tCGCGCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg > 2:1656479/1‑76 (MQ=255) cgcgCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc < 1:820771/76‑1 (MQ=255) cgcgCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc > 1:698641/1‑76 (MQ=255) cgcgCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc > 2:960755/1‑76 (MQ=255) cgcgCAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGc > 1:634063/1‑76 (MQ=255) gcgcAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCa > 1:655224/1‑76 (MQ=255) gcgcAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCa > 2:435025/1‑76 (MQ=255) gcgcAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCa < 2:587673/76‑1 (MQ=255) cAATGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCg > 2:160113/1‑76 (MQ=255) aaTGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGa < 2:674643/76‑1 (MQ=255) tGGCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTATCATCCCGGCAAAGAACGCACCGAGt > 2:105553/1‑76 (MQ=255) gCAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGc < 2:636129/76‑1 (MQ=255) cAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCa < 1:1868003/76‑1 (MQ=255) cAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCa < 2:1221347/76‑1 (MQ=255) cAGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCa < 1:450334/76‑1 (MQ=255) aGCGCATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa < 1:225740/76‑1 (MQ=255) gcgcATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaaa < 2:1732835/76‑1 (MQ=255) | AGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAA > REL606/474413‑474558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |