BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,282,445	C→T	A163T (GCC→ACC)	yjcR ←	predicted membrane fusion protein of efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,282,445	0	C	T	100.0%	194.2 / NA	50	A163T (GCC→ACC)	yjcR	predicted membrane fusion protein of efflux pump
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/28); total (22/28)

ATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTG  >  REL606/4282374‑4282515

aTCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGgtgcgc                                                                    >  2:981153/1‑76 (MQ=255)

  ccgcgcggcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGCCATTAAGATCCGCTTCTGCGGTGCGCTg                                                                  <  2:900327/73‑1 (MQ=255)

  ccACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTg                                                                  <  2:1534895/76‑1 (MQ=255)

   cACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTgc                                                                 >  1:148703/1‑76 (MQ=255)

    ccgccgcTGGCGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTgcg                                                                <  1:1380705/75‑1 (MQ=255)

     cgccgcTGACGGCGCTGGCGGCTGCCTGCGCCTGTAACAATATGGCATTCAGATCCGCTTCCgcggcgcgcggcgc                                                               >  2:1797834/1‑76 (MQ=255)

         gcTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGtt                                                           >  2:1132945/1‑76 (MQ=255)

            gACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGGTCtt                                                        >  1:1617818/1‑76 (MQ=255)

             aCGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTg                                                       <  1:1184899/76‑1 (MQ=255)

             aCGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTg                                                       <  2:1852859/76‑1 (MQ=255)

             aCGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTg                                                       <  2:1121720/76‑1 (MQ=255)

                gcgcGGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCAc                                                    <  2:1022404/76‑1 (MQ=255)

                 cgcTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACg                                                   <  1:1791916/76‑1 (MQ=255)

                  gcTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACgg                                                  <  2:763272/76‑1 (MQ=255)

                     ggcggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCa                                               >  1:1742019/1‑76 (MQ=255)

                     ggcggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCa                                               >  1:1757312/1‑76 (MQ=255)

                     ggcggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCa                                               <  2:100957/76‑1 (MQ=255)

                      gcggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCaa                                              >  1:96474/1‑76 (MQ=255)

                      gcggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCaa                                              >  1:72669/1‑76 (MQ=255)

                       cggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAAc                                             <  2:165899/76‑1 (MQ=255)

                         ncTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACAt                                           >  1:208750/2‑76 (MQ=255)

                         gcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACAt                                           <  1:666287/76‑1 (MQ=255)

                         gcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACAt                                           >  2:309156/1‑76 (MQ=255)

                          cTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATc                                          <  2:868288/76‑1 (MQ=255)

                            gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCtt                                        <  1:821669/76‑1 (MQ=255)

                            gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCtt                                        <  2:1428981/76‑1 (MQ=255)

                            gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCtt                                        <  2:216112/76‑1 (MQ=255)

                            gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCtt                                        >  1:209462/1‑76 (MQ=255)

                            gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCtt                                        >  1:2047034/1‑76 (MQ=255)

                             aCTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTc                                       >  2:1806978/1‑76 (MQ=255)

                               tGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCg                                     <  2:1033101/76‑1 (MQ=255)

                               tGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCg                                     <  2:1818720/76‑1 (MQ=255)

                                  gcCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTg                                  <  1:2051700/76‑1 (MQ=255)

                                    cTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTgag                                <  1:298095/76‑1 (MQ=255)

                                    cTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTgag                                >  2:670504/1‑76 (MQ=255)

                                    cTGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTgag                                >  1:486088/1‑76 (MQ=255)

                                     tGTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTgaga                               <  1:940343/76‑1 (MQ=255)

                                      gTAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGAc                              <  1:1450642/76‑1 (MQ=255)

                                       tAACAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACa                             >  1:1065248/1‑76 (MQ=255)

                                          cAATACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACaaaa                          <  2:1375323/76‑1 (MQ=255)

                                           aaTACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAAc                         >  2:1508648/1‑76 (MQ=255)

                                            aTACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAAcc                        >  2:1640385/1‑76 (MQ=255)

                                            aTACGGCATTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAAcc                        >  1:1542148/1‑76 (MQ=255)

                                                   aTTAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTc                 <  1:1747183/76‑1 (MQ=255)

                                                     tAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAg               <  1:874715/76‑1 (MQ=255)

                                                     tAAGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAg               <  1:795886/76‑1 (MQ=255)

                                                       aGATCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTa             >  2:1205114/1‑76 (MQ=255)

                                                          tCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATg          <  2:1426288/76‑1 (MQ=255)

                                                          tCCGCTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATg          >  2:720714/1‑76 (MQ=255)

                                                              cTTCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTc      >  2:1216595/1‑76 (MQ=255)

                                                               ttCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCg     <  2:319816/76‑1 (MQ=255)

                                                               ttCTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCg     <  2:417641/76‑1 (MQ=255)

                                                                 cTGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGt   >  1:244395/1‑76 (MQ=255)

                                                                  tGCGGTGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTg  >  1:928445/1‑76 (MQ=255)

ATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTG  >  REL606/4282374‑4282515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`