Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	980,314	A→C	K410Q (AAA→CAA)	rpsA →	30S ribosomal protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	980,314	0	A	C	100.0%	186.8 / NA	46	K410Q (AAA→CAA)	rpsA	30S ribosomal protein S1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/25);  total (21/25)

CGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGCGTTA  >  REL606/980241‑980386                                                                          |                                                                         cggcATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACcaa                                                                        <  1:1402531/76‑1 (MQ=255)  ggcATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACcaaa                                                                       <  1:767297/76‑1 (MQ=255)       cGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGc                                                                  >  1:531280/1‑76 (MQ=255)        gACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGcg                                                                 <  1:1643581/76‑1 (MQ=255)           ggCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGcgacg                                                              <  1:1202290/76‑1 (MQ=255)            gCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGcgacga                                                             >  1:362337/1‑76 (MQ=255)            gCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGcgacga                                                             >  1:1719887/1‑76 (MQ=255)            gCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGcgacga                                                             <  2:1573004/76‑1 (MQ=255)              cTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGaaa                                                           >  2:534051/1‑76 (MQ=255)               tGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAAt                                                          >  2:730782/1‑76 (MQ=255)               tGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAAt                                                          >  1:1564242/1‑76 (MQ=255)                 gTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCg                                                        >  1:630099/1‑76 (MQ=255)                   tCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCt                                                      <  1:1847403/76‑1 (MQ=255)                    cACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTg                                                     <  2:687371/76‑1 (MQ=255)                     aCCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGc                                                    <  2:585844/76‑1 (MQ=255)                           cTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAgttgt                                              <  2:1675704/76‑1 (MQ=255)                             gACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTc                                            <  2:603560/76‑1 (MQ=255)                                aTCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGc                                         >  1:1628415/1‑76 (MQ=255)                                aTCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGc                                         >  1:1541422/1‑76 (MQ=255)                                aTCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGc                                         >  1:1431798/1‑76 (MQ=255)                                aTCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGc                                         <  1:475491/76‑1 (MQ=255)                                 tctcCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCa                                        <  2:1968381/76‑1 (MQ=255)                                   tcCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAgg                                      <  2:1508617/76‑1 (MQ=255)                                    ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGt                                     >  2:617420/1‑76 (MQ=255)                                    ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGt                                     >  1:1158204/1‑76 (MQ=255)                                    ccTGGAACGTGGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGt                                     <  2:377391/76‑1 (MQ=255)                                     cTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGtt                                    >  2:284149/1‑76 (MQ=255)                                      tGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTg                                   >  1:1559672/1‑76 (MQ=255)                                      tGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTg                                   <  1:1554659/76‑1 (MQ=255)                                      tGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTg                                   <  2:1704463/76‑1 (MQ=255)                                         aaCGTGGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACg                                <  2:809884/76‑1 (MQ=255)                                             ttGCAGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAga                            >  1:983726/1‑76 (MQ=255)                                                 aGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgt                        <  2:1094535/76‑1 (MQ=255)                                                 aGGCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgt                        <  1:1492674/76‑1 (MQ=255)                                                   gCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtga                      >  2:529449/1‑76 (MQ=255)                                                   gCGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtga                      <  1:1486380/76‑1 (MQ=255)                                                    cGAAGAAGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaa                     <  1:223739/76‑1 (MQ=255)                                                     gaagaaGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaac                    >  2:836773/1‑76 (MQ=255)                                                     gaagaaGCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaac                    <  1:1284369/76‑1 (MQ=255)                                                      aagaagCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaacg                   <  2:1141298/76‑1 (MQ=255)                                                      aagaagCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaacg                   >  1:1575790/1‑76 (MQ=255)                                                        gaagCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTa                 >  2:1481152/1‑76 (MQ=255)                                                         aagCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTAt                <  1:498107/76‑1 (MQ=255)                                                         aagCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTAt                <  1:160481/76‑1 (MQ=255)                                                          agCAGTTCGTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTAtc               <  2:626931/76‑1 (MQ=255)                                                                  gTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTggg       >  1:556411/1‑76 (MQ=255)                                                                  gTGAATACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTggg       >  1:1884719/1‑76 (MQ=255)                                                                       tACCAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGCGTTa  <  1:1523318/76‑1 (MQ=255)                                                                          |                                                                         CGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGCGTTA  >  REL606/980241‑980386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑