| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,769 | C→T | intergenic (+100/‑95) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,769 | 0 | C | T | 96.0% | 267.2 / 1.5 | 74 | intergenic (+100/‑95) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (37/34); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
TCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCAT > REL606/3145697‑3145840 | tCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTggtggt < 2:401913/76‑1 (MQ=255)tCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTggtggt < 2:780751/76‑1 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTa < 1:851896/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 2:1428270/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 2:187781/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 1:223667/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTaa > 1:1173067/1‑76 (MQ=255) tgAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaaa > 2:925684/2‑76 (MQ=255) ggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaaa < 1:1413988/76‑1 (MQ=255) gAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaac < 2:1373087/76‑1 (MQ=255) aGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaacaa < 2:1915939/76‑1 (MQ=255) gCCCCCGACAGCCGCACTGAGAGACAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaacaac > 2:344676/1‑76 (MQ=255) cccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAAcc > 2:1675016/1‑76 (MQ=255) cccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAAcc < 1:1580877/76‑1 (MQ=255) cGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACACCCTCGTAATTATCGTTGGTGGTAAACAACCGTTg > 1:2053835/1‑76 (MQ=255) aGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAtt < 2:858003/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTc < 2:1929335/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTc < 1:2071920/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTc < 2:582485/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTc < 2:571805/76‑1 (MQ=255) gCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAg < 1:1651491/76‑1 (MQ=255) gCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAg < 1:1651482/76‑1 (MQ=255) cACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGc < 1:185672/76‑1 (MQ=255) tGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGtt > 1:335762/1‑76 (MQ=255) gagaGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTa > 2:1947221/1‑76 (MQ=255) gagaGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTa > 2:1730243/1‑76 (MQ=255) agagGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTaa > 2:1727245/1‑76 (MQ=255) gagGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAAc < 1:1012140/76‑1 (MQ=255) agGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACg > 2:447431/1‑76 (MQ=255) cGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGaa < 1:554839/76‑1 (MQ=255) ggCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAg > 2:556531/1‑76 (MQ=255) ggCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAg < 1:884715/76‑1 (MQ=255) ggCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAg > 1:717046/1‑76 (MQ=255) cAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGa > 2:1003604/1‑76 (MQ=255) cAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGa > 2:192196/1‑76 (MQ=255) aaaTATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAc > 2:1923284/1‑76 (MQ=255) atatatAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACAt > 1:1027746/1‑76 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 2:102296/76‑1 (MQ=255) atataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTACCGGCTGAAGGACATCg > 1:1646548/1‑76 (MQ=255) atataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 2:827730/1‑76 (MQ=255) tataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 1:551447/76‑1 (MQ=255) tataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 1:169560/76‑1 (MQ=255) ataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg > 1:757418/1‑76 (MQ=255) ataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg < 2:1447220/76‑1 (MQ=255) ataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg > 2:831830/1‑76 (MQ=255) taAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGt > 1:502150/1‑76 (MQ=255) aGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa < 1:766829/76‑1 (MQ=255) aGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa < 2:1242282/76‑1 (MQ=255) aGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa < 2:227689/76‑1 (MQ=255) aGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa > 2:333142/1‑76 (MQ=255) aCGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATc > 1:571020/1‑76 (MQ=255) cGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCg < 1:925684/76‑1 (MQ=255) cGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCg < 1:834660/76‑1 (MQ=255) gCCCCCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGc > 1:73092/1‑76 (MQ=255) cccTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGcc > 2:180581/1‑76 (MQ=255) cTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCa > 1:2049592/1‑76 (MQ=255) tCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCaa > 2:1074155/1‑76 (MQ=255) gTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAaca > 2:164172/1‑76 (MQ=255) gTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAaca > 1:1729906/1‑76 (MQ=255) gTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAaca < 2:1244965/76‑1 (MQ=255) gTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAaca > 1:1428385/1‑76 (MQ=255) tAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAacac > 1:1554567/1‑76 (MQ=255) tAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAacac > 2:1088198/1‑76 (MQ=255) tAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAacac > 1:1414013/1‑76 (MQ=255) aTTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCa > 1:1020687/1‑76 (MQ=255) aTTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCa < 2:1985427/76‑1 (MQ=255) aTTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCa < 2:1605602/76‑1 (MQ=255) aTTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCa < 2:1515887/76‑1 (MQ=255) ttATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCaa > 2:999068/1‑76 (MQ=255) ttATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCac > 2:48865/1‑75 (MQ=255) tATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAAACGCCCAACACCAAc < 2:2053835/76‑1 (MQ=255) aTCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAAcc < 2:1033088/76‑1 (MQ=255) tCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCt > 2:568858/1‑76 (MQ=255) cGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTc > 2:80732/1‑76 (MQ=255) cGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTc > 1:1782092/1‑76 (MQ=255) cGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTc > 2:942351/1‑76 (MQ=255) ttGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCAt < 1:630006/76‑1 (MQ=255) | TCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCAT > REL606/3145697‑3145840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |