| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,842,751 | A→C | L447W (TTG→TGG) | dnaA ← | chromosomal replication initiation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,842,751 | 0 | A | C | 95.2% | 256.6 / 1.5 | 62 | L447W (TTG→TGG) | dnaA | chromosomal replication initiation protein |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base C (32/27); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01 | |||||||||||
CATAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCGCCAATCTCCG > REL606/3842680‑3842823 | naTAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTg > 1:341076/2‑76 (MQ=255)cATAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTg < 1:574549/76‑1 (MQ=255) tAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCt < 2:1577103/76‑1 (MQ=255) tAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCt > 1:354489/1‑76 (MQ=255) tAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCt < 1:84878/76‑1 (MQ=255) aGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTc < 1:1436153/76‑1 (MQ=255) ggTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCg < 2:1763339/76‑1 (MQ=255) gTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGa > 2:1146867/1‑76 (MQ=255) ttACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATc > 1:1535023/1‑76 (MQ=255) aCGATGATAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCtt > 1:431498/1‑76 (MQ=255) aCGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCtt < 2:647408/76‑1 (MQ=255) cGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTa > 2:2042317/1‑76 (MQ=255) gATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTAc < 1:477456/76‑1 (MQ=255) aTGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACg < 2:103896/76‑1 (MQ=255) aTGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACg < 1:207625/76‑1 (MQ=255) tGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACgg < 1:127133/76‑1 (MQ=255) tGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACgg < 1:466090/76‑1 (MQ=255) gACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTTCTCGATCTTACggc < 2:92729/76‑1 (MQ=255) gACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACggc > 1:1738523/1‑76 (MQ=255) aCAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACggca < 2:354489/76‑1 (MQ=255) aTGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACggcaggc > 2:912806/1‑76 (MQ=255) tGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACggcaggca > 2:1298780/1‑76 (MQ=255) tGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAg > 1:1023274/1‑76 (MQ=255) tGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAg > 1:1746357/1‑76 (MQ=255) gATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGc < 2:1480503/76‑1 (MQ=255) aTTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCa > 2:613012/1‑76 (MQ=255) aTTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCa > 1:470601/1‑76 (MQ=255) tAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCCTACGGCAGGCATGAAGCAcc > 1:499590/1‑76 (MQ=255) aaaTTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACcg > 1:1121682/1‑76 (MQ=255) aTTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACcgtc < 2:1175661/76‑1 (MQ=255) ttGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACcgtcgt > 1:1314070/1‑76 (MQ=255) ttGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACcgtcgt > 2:1292205/1‑76 (MQ=255) ttGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACcgtcgt > 1:82217/1‑76 (MQ=255) gAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtgg > 1:608818/1‑75 (MQ=255) aaaaaTCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGGCgtggg > 1:1129938/1‑74 (MQ=255) aaTCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTc < 2:490468/76‑1 (MQ=255) aTCTTCTTTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCa < 1:624430/76‑1 (MQ=255) tcttctTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCAc < 1:1140754/76‑1 (MQ=255) cttcttTGATATCCTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACg < 1:1248168/76‑1 (MQ=255) cttTGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑cc > 2:760309/1‑76 (MQ=255) tGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGGATGAAGCACCGTCGTGTGGTCACGG‑ccccc > 2:598187/1‑76 (MQ=255) tGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑ccacc < 1:480469/76‑1 (MQ=255) tGATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑ccacc > 2:1856775/1‑76 (MQ=255) gATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑ccacca < 1:887944/76‑1 (MQ=255) gATATCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑ccacca < 2:988119/76‑1 (MQ=255) atatCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑ccaccca > 2:1068090/1‑74 (MQ=255) atatCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACCG‑ccaccca > 1:1055507/1‑74 (MQ=255) tatCGTGGCTCTCTTCACGCCACTGCTCGATCTTGCGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCaaa < 1:1942254/76‑1 (MQ=255) tatCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCaaa < 2:476945/76‑1 (MQ=255) atCGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAAc < 2:383978/76‑1 (MQ=255) acngTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAAc > 2:1464089/4‑76 (MQ=255) cGTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGc < 2:99782/76‑1 (MQ=255) cGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCCTGAAGCACCGTCGTGGGGGCCCGGCCCACCaaagc > 2:1222127/1‑74 (MQ=255) gTGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCa < 1:1390695/76‑1 (MQ=255) tGGCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCAt > 1:1765348/1‑76 (MQ=255) gCTCTCTTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCg < 2:907392/76‑1 (MQ=255) ctctTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCGCCa > 1:34357/1‑76 (MQ=255) ctctTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGGCACGG‑CCACCCAACGCATCGccc > 1:1231769/1‑75 (MQ=255) ctTCACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCGCCAAt > 1:483791/1‑76 (MQ=255) tanaCGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGGCACGG‑CCACCCAACGCATCGCCCAtc > 2:1466488/4‑76 (MQ=255) cACGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGGGGGCACGG‑CCCCCCAAAGCAACGCCAAactc > 1:245240/1‑76 (MQ=255) cGCCACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCGCCAATCTCCg < 2:1686497/76‑1 (MQ=255) | CATAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGG‑CCACCAAACGCATCGCCAATCTCCG > REL606/3842680‑3842823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |