breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,393,061	0	T	G	52.0%	40.2 / 81.1	50	V211G (GTG→GGG)	aidB	isovaleryl CoA dehydrogenase
*	REL606	2,296,329	0	A	C	48.5%	57.7 / 49.7	66	intergenic (‑149/‑66)	inaA/yfaH	hypothetical protein/hypothetical protein
*	REL606	3,492,082	0	T	G	40.6%	96.2 / 40.1	69	V490G (GTG→GGG)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*	REL606	2,631,772	0	A	C	40.0%	66.9 / 23.9	50	intergenic (‑267/‑141)	rpoE/nadB	RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*	REL606	2,231,892	0	A	C	37.8%	48.1 / 17.0	37	T25P (ACC→CCC)	yejH	predicted ATP‑dependet helicase
*	REL606	3,691,844	0	T	G	36.7%	81.1 / 13.6	50	N226T (AAC→ACC)	yiaY	predicted Fe‑containing alcohol dehydrogenase
*	REL606	1,875,483	0	G	C	32.7%	192.0 / 114.6	112	intergenic (+156/‑28)	yeaB/sdaA	predicted NUDIX hydrolase/L‑serine deaminase I
*	REL606	154,497	0	T	G	31.2%	163.0 / 18.2	77	A191A (GCA→GCC)	yadL	predicted fimbrial‑like adhesin protein
*	REL606	1,004,106	0	A	C	30.2%	119.5 / 10.5	53	intergenic (‑32/+570)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*	REL606	1,747,903	0	G	T	28.4%	223.0 / 68.8	88	A8S (GCA→TCA)	ydiL	hypothetical protein
*	REL606	4,169,232	0	A	T	28.1%	138.2 / 14.6	57	intergenic (+48/‑165)	rpoC/htrC	DNA‑directed RNA polymerase subunit beta'/heat shock protein
*	REL606	4,574,252	0	T	G	27.9%	91.3 / 11.0	43	S5S (TCA→TCC)	hpaX	hypothetical 4‑hydroxyphenylacetate permease
*	REL606	1,688,990	0	A	C	27.0%	143.7 / 11.4	63	T92P (ACC→CCC)	nth	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*	REL606	1,029,711	0	G	C	24.1%	73.5 / 10.2	29	R207P (CGC→CCC)	pqiA	paraquat‑inducible membrane protein A
*	REL606	2,417,495	0	T	A	24.0%	205.7 / 20.2	75	L24F (TTA→TTT)	emrK	EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
*	REL606	3,733,719	0	C	G	22.4%	124.1 / 12.5	49	G63G (GGC→GGG)	gumD	ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2038081	27 (0.410)	3 (0.050)	3/134	7.8	10.0%	coding (50/1176 nt)	wzy	O antigen polymerase (Wzy)
	?	REL606	= 2038277	30 (0.510)					coding (672/822 nt)	wbbB	putative glycosyltransferase WbbB