| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 990,162 | T→G | E111A (GAA→GCA) | ycbC ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 990,162 | 0 | T | G | 97.7% | 174.0 / ‑3.2 | 43 | E111A (GAA→GCA) | ycbC | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (21/21); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
TATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCAC > REL606/990091‑990234 | tATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtgcatt > 1:1027766/1‑76 (MQ=255) aTTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtgcattc > 1:1725667/1‑76 (MQ=255) aTTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtgcattc < 2:1451005/76‑1 (MQ=255) aTTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtgcattc > 1:1704035/1‑76 (MQ=255) ttGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtgcattca < 1:1697147/76‑1 (MQ=255) tGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAg > 2:1027070/1‑76 (MQ=255) gTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGc < 2:1123982/76‑1 (MQ=255) tttttGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCg > 2:1711680/1‑76 (MQ=255) tttGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTg < 2:1478888/76‑1 (MQ=255) tttGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTg < 2:1310611/76‑1 (MQ=255) ttGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTgg > 2:907360/1‑76 (MQ=255) tGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGc > 1:943912/1‑76 (MQ=255) tACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGa > 2:1733052/1‑76 (MQ=255) aCGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGAc > 1:478411/1‑76 (MQ=255) gCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTg < 2:799355/76‑1 (MQ=255) cGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTg < 1:11292/76‑1 (MQ=255) cGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTg > 2:1554607/1‑76 (MQ=255) gTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGa > 1:385829/1‑76 (MQ=255) aaGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTa > 1:258862/1‑76 (MQ=255) aTCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAAt < 1:239597/76‑1 (MQ=255) tCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAAtt < 1:1706532/76‑1 (MQ=255) tCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAAtt < 1:790102/76‑1 (MQ=255) cAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa < 2:1745333/76‑1 (MQ=255) cAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa < 2:502925/76‑1 (MQ=255) tgTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTag > 2:1877813/2‑76 (MQ=255) aGTTTTGATCCCGGATTTGCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTag < 1:875313/76‑1 (MQ=255) tttGATCCCGGATTTTCGCGCCATAGGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGc > 2:600016/1‑76 (MQ=255) tttGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGc < 2:404503/76‑1 (MQ=255) ttGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCt < 1:1793706/76‑1 (MQ=255) tGATCCCGGATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTc < 1:429416/76‑1 (MQ=255) ggATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGccc > 1:648975/1‑76 (MQ=255) ggATTTTCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGccc > 1:1779376/1‑76 (MQ=255) ttttCGCGCCATAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACt > 2:650476/1‑76 (MQ=255) aTAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCa < 1:1343528/76‑1 (MQ=255) aTAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCa < 1:277413/76‑1 (MQ=255) aTAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCa < 1:408073/76‑1 (MQ=255) tAAGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAg < 1:1222769/76‑1 (MQ=255) aaGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAgg > 1:1671790/1‑76 (MQ=255) aGCGAATACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGt < 2:51872/76‑1 (MQ=255) aaTACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGtatct > 2:182605/1‑74 (MQ=255) aaTACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATaa < 2:1737265/76‑1 (MQ=255) aaTACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATaa > 2:1643313/1‑76 (MQ=255) aaTACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCAAGGCATaa > 1:216029/1‑76 (MQ=255) aTACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAac > 2:1558547/1‑76 (MQ=255) aTACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAac > 1:1793746/1‑76 (MQ=255) tACCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAacc > 1:91912/1‑76 (MQ=255) aCCTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAacca > 1:1746777/1‑76 (MQ=255) ccTGCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAaccac < 2:1441213/76‑1 (MQ=255) | TATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTAGAGCTCGGTGCCCACTGCGGGTTCCAGGTATAACCAC > REL606/990091‑990234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |