| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,100,824 | A→T | I136N (ATC→AAC) | hslU ← | ATP‑dependent protease ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,100,824 | 0 | A | T | 97.8% | 164.5 / ‑4.4 | 45 | I136N (ATC→AAC) | hslU | ATP‑dependent protease ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (16/28); total (16/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGAATGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGATCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTGGACG > REL606/4100753‑4100895 | ggAATGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAg < 1:758354/76‑1 (MQ=255)ggAATGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAg < 2:804707/76‑1 (MQ=255) aaTGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCa < 1:1081699/76‑1 (MQ=255) tGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACg > 1:740299/1‑76 (MQ=255) tGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACg > 1:1579446/1‑76 (MQ=255) ccTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTc < 2:609778/76‑1 (MQ=255) tGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCga > 2:64671/1‑76 (MQ=255) gACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCgag > 1:522298/1‑76 (MQ=255) gACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCgag < 2:1804082/76‑1 (MQ=255) ggACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGttct < 2:1290117/76‑1 (MQ=255) ggACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGttct < 1:623909/76‑1 (MQ=255) tCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCa > 2:218201/1‑76 (MQ=255) tCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCa > 1:1060075/1‑76 (MQ=255) tgctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGtt < 2:1530893/76‑1 (MQ=255) tgctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGtt < 2:1515905/76‑1 (MQ=255) tgctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGtt < 1:1395932/76‑1 (MQ=255) gctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCCGttc > 1:703845/1‑76 (MQ=255) ctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGttct > 2:926982/1‑76 (MQ=255) ctgctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGttct < 2:1515781/76‑1 (MQ=255) gctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGttcttc < 2:630454/76‑1 (MQ=255) gctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGttcttc < 2:1393230/76‑1 (MQ=255) ctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCa < 2:989158/76‑1 (MQ=255) ctgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCa > 2:261641/1‑76 (MQ=255) tgTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAg < 1:261641/76‑1 (MQ=255) gTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAgc < 2:241560/76‑1 (MQ=255) ggTCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGa < 1:80358/76‑1 (MQ=255) tCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATa > 2:1117733/1‑76 (MQ=255) tCTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATa < 1:1424827/76‑1 (MQ=255) cTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATaa < 1:1057621/76‑1 (MQ=255) cTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATaa < 2:835783/76‑1 (MQ=255) cTGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATaa > 1:242991/1‑76 (MQ=255) tGTCCCCAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAAc < 2:484569/76‑1 (MQ=255) ccccAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGt > 1:1296172/1‑76 (MQ=255) ccAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGttt < 2:1089145/76‑1 (MQ=255) cAGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGtttt > 2:1139442/1‑76 (MQ=255) aGTTGTTTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGttttt < 2:950618/76‑1 (MQ=255) gttTTTAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCg < 2:366385/76‑1 (MQ=255) ttttAGCAGGTGGGATCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGAt < 1:275964/76‑1 (MQ=255) tAGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGc > 2:1574675/1‑76 (MQ=255) aGCAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGcc > 1:478885/1‑76 (MQ=255) cAGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTg < 2:516687/76‑1 (MQ=255) aGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTgg < 1:1610457/76‑1 (MQ=255) aGGTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTgg < 1:524595/76‑1 (MQ=255) ggTGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTGGa > 1:1830893/1‑76 (MQ=255) tGGGTTCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTGGAcg < 1:1420533/76‑1 (MQ=255) | GGAATGCCTGACGAGCAGCGGACGGTTCCTGCTGCTGTTCGGTCTGTCCCCAGTTGTTTTTAGCAGGTGGGATCAGCACGTCGAGAATACGTTCTTCTGCCAGTTCTTCAGCGCGATAACGGTTTTTCTCGATAGCCTGGACG > REL606/4100753‑4100895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |